CAJ | 학술논문

目的：探讨乙酸铅对大鼠大脑皮质、小脑、海马组织自由基和脂质过氧化的影响。方法48只刚断乳雄性SD大鼠，按体质量采用随机区组法分为4组（对照组和低、中、高剂量组），对照组大鼠饮用去离子水，低、中、高剂量组分别饮用200、400、800 mg/L的乙酸铅溶液。连续染毒60 d后，取血进行血铅测定，取各组脑组织（大脑皮质、小脑和海马）进行羟自由基抑制活力、过氧化氢（H2O2）水平及丙二醛（MDA）含量的测定。结果与对照组相比，各染铅组大鼠血铅含量显著升高，大脑皮质、小脑和海马组织羟自由基抑制活力明显下降，且随染铅剂量的升高呈逐渐下降趋势（P<0.05）；大脑皮质、小脑和海马组织H2O2水平及MDA含量高于对照组，并随着染铅剂量的升高呈逐渐升高趋势（P<0.05）；大脑皮质、小脑和海马组织的羟自由基抑制活力与血铅含量呈负相关（r分别为-0.505、-0.414、-0.448，P<0.05），H2O2和MDA含量均与血铅含量呈正相关（r分别为0.301、0.411、0.378和0.404、0.324、0.510，P<0.05）。结论铅可通过诱发自由基产生，导致大鼠脑组织发生脂质过氧化反应。
목적：탐토을산연대대서대뇌피질、소뇌、해마조직자유기화지질과양화적영향。방법48지강단유웅성SD대서，안체질량채용수궤구조법분위4조（대조조화저、중、고제량조），대조조대서음용거리자수，저、중、고제량조분별음용200、400、800 mg/L적을산연용액。련속염독60 d후，취혈진행혈연측정，취각조뇌조직（대뇌피질、소뇌화해마）진행간자유기억제활력、과양화경（H2O2）수평급병이철（MDA）함량적측정。결과여대조조상비，각염연조대서혈연함량현저승고，대뇌피질、소뇌화해마조직간자유기억제활력명현하강，차수염연제량적승고정축점하강추세（P<0.05）；대뇌피질、소뇌화해마조직H2O2수평급MDA함량고우대조조，병수착염연제량적승고정축점승고추세（P<0.05）；대뇌피질、소뇌화해마조직적간자유기억제활력여혈연함량정부상관（r분별위-0.505、-0.414、-0.448，P<0.05），H2O2화MDA함량균여혈연함량정정상관（r분별위0.301、0.411、0.378화0.404、0.324、0.510，P<0.05）。결론연가통과유발자유기산생，도치대서뇌조직발생지질과양화반응。
Objective To explore the effects of lead acetate on free radicals and lipid peroxidation in the cerebral cor?tex, cerebellum, and hippocampus in rat brains. Methods SD rats (n=48), who were just weaned, were randomly divided in?to 4 groups base on their weight. Then the rats were fed with lead acetate in drinking water at the final concentrations of 0 mg/L (deionized water), 200 mg/L, 400 mg/L, 800 mg/L respectively. Blood lead level as well as the hydroxyl free radical inhibiting activity, the levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in cerebral cortex, cerebellum, and hippocam?pus were measured 60 days after lead contamination in water. Results Upon lead exposure, blood lead levels increased sig?nificantly as compared with the control. The hydroxyl free radical inhibiting activity in cerebral cortex, cerebellum, and hip?pocampus decreased significantly in a dose dependent manner of lead(P < 0.05). And they all correlated negatively with blood lead level (r=-0.505,-0.414,-0.448, P<0.05). By contrast, blood lead level was positively correlated with H2O2 and MDA in these brain tissues (r=0.301, 0.411, 0.378, and 0.404, 0.324, 0.510,P < 0.05). Conclusion Lead exposure can lead to lipid peroxidation of rat brain tissues through inducing free radicals.