CAJ | 학술논문

目的：本研究旨在探讨慢性阻塞性肺疾病（COPD）患者离体肺段肺泡液体清除率（AFC）的改变及其与环磷酸鸟苷（cGMP）依赖性蛋白激酶2（PKG2）的关系。方法应用临床外科手术肺切除患者的肺段标本，将药物通过插管注入远端肺组织。应用考马斯亮兰法测定肺泡液体内小牛血清白蛋白浓度的方法测定人离体肺段AFC。应用相关酶联免疫试剂盒检测PKG2。结果 COPD患者肺组织 AFC增加，PKG2明显高于对照组。结论 COPD患者气道黏液分泌增多肺脏液体清除作用增强，其机制可能与上皮钠通道功能增强有关。
목적：본연구지재탐토만성조새성폐질병（COPD）환자리체폐단폐포액체청제솔（AFC）적개변급기여배린산조감（cGMP）의뢰성단백격매2（PKG2）적관계。방법응용림상외과수술폐절제환자적폐단표본，장약물통과삽관주입원단폐조직。응용고마사량란법측정폐포액체내소우혈청백단백농도적방법측정인리체폐단AFC。응용상관매련면역시제합검측PKG2。결과 COPD환자폐조직 AFC증가，PKG2명현고우대조조。결론 COPD환자기도점액분비증다폐장액체청제작용증강，기궤제가능여상피납통도공능증강유관。
Objective To explore the changes of human alveolar fluid clearance ex vivo in chronic obstructive pulmonary disease(COPD) patients ,and to determine its relationship with human cGMP‐dependent protein kinase 2(PKG2).Methods Human lungs were obtained from patients who underwent pneumonectomy ,and drugs were instilled into the distal air spaces through the catheter .We estimated alveolar fluid clearance by the changes of pro‐tein concentrations in aspirated solutions measured by Bradford method .Human PKG2 was calculated by the rela‐tive ELISA Kit.Results Human alveolar fluid clearance was increased in COPD patients ,and the levels of PKG2 were much higher than those of control .Conclusion These observations suggest that alveolar fluid clearance was enhanced in COPD patients ,which may be related to the augment of epithelial sodium channel.