实用检验医师杂志
實用檢驗醫師雜誌
실용검험의사잡지
Chinese Journal of Laboratory Pathologist
2015年
3期
169-172
,共4页
陈立%冯学泉%万晨光%左娜%穆红
陳立%馮學泉%萬晨光%左娜%穆紅
진립%풍학천%만신광%좌나%목홍
脑死亡%白细胞介素-8%炎性反应%肾功能
腦死亡%白細胞介素-8%炎性反應%腎功能
뇌사망%백세포개소-8%염성반응%신공능
Brain dead%Interleukin-8%Inflammatory response%Renal function
目的：探讨白细胞介素-8（interleukin-8， IL-8）水平在猪脑死亡模型的变化及其与肾功能指标的相关性。方法采用标准实验长白小猪7头，手术准备操作后取脑死亡前静脉血，硬膜外颅内生理盐水加压至脑死亡，分别在确立脑死亡时间点及脑死亡后3h、6h、9h取静脉血。以免疫芯片荧光定量法检测脑死亡前、脑死亡0h、脑死亡后3h、6h、9h血清IL-8浓度，以生物化学法检测尿素、肌酐（creatinine， Cr）水平，对检测结果进行统计学分析。结果脑死亡后IL-8检测结果开始升高，3 h、6 h、9 h的IL-18检测结果均高于脑死亡前，且差异均有统计学意义（P均＜0.05）。脑死亡后各时间点IL-8的检测结果差异无统计学意义（H＝7.840，P＝0.098）。脑死亡后3 h、6 h、9 h的尿素检测结果均高于脑死亡前，差异均有统计学意义（P均＜0.05）。脑死亡后6 h、9 h的Cr检测结果均高于脑死亡前，差异均有统计学意义（P均＜0.05）。脑死亡前后血清IL-8与尿素检测结果呈正相关性（r＝0.453，P＝0.012），与Cr检测结果无相关性（r＝0.209，P＝0.267）。结论脑死亡可引起组织释放IL-8，并可造成组织细胞及肾功能不可逆性损伤。因此，临床为保护潜在移植器官应在脑死亡确立以后及时采取有效的血液净化吸附IL-8，以降低组织器官的炎性损伤。
目的：探討白細胞介素-8（interleukin-8， IL-8）水平在豬腦死亡模型的變化及其與腎功能指標的相關性。方法採用標準實驗長白小豬7頭，手術準備操作後取腦死亡前靜脈血，硬膜外顱內生理鹽水加壓至腦死亡，分彆在確立腦死亡時間點及腦死亡後3h、6h、9h取靜脈血。以免疫芯片熒光定量法檢測腦死亡前、腦死亡0h、腦死亡後3h、6h、9h血清IL-8濃度，以生物化學法檢測尿素、肌酐（creatinine， Cr）水平，對檢測結果進行統計學分析。結果腦死亡後IL-8檢測結果開始升高，3 h、6 h、9 h的IL-18檢測結果均高于腦死亡前，且差異均有統計學意義（P均＜0.05）。腦死亡後各時間點IL-8的檢測結果差異無統計學意義（H＝7.840，P＝0.098）。腦死亡後3 h、6 h、9 h的尿素檢測結果均高于腦死亡前，差異均有統計學意義（P均＜0.05）。腦死亡後6 h、9 h的Cr檢測結果均高于腦死亡前，差異均有統計學意義（P均＜0.05）。腦死亡前後血清IL-8與尿素檢測結果呈正相關性（r＝0.453，P＝0.012），與Cr檢測結果無相關性（r＝0.209，P＝0.267）。結論腦死亡可引起組織釋放IL-8，併可造成組織細胞及腎功能不可逆性損傷。因此，臨床為保護潛在移植器官應在腦死亡確立以後及時採取有效的血液淨化吸附IL-8，以降低組織器官的炎性損傷。
목적：탐토백세포개소-8（interleukin-8， IL-8）수평재저뇌사망모형적변화급기여신공능지표적상관성。방법채용표준실험장백소저7두，수술준비조작후취뇌사망전정맥혈，경막외로내생리염수가압지뇌사망，분별재학립뇌사망시간점급뇌사망후3h、6h、9h취정맥혈。이면역심편형광정량법검측뇌사망전、뇌사망0h、뇌사망후3h、6h、9h혈청IL-8농도，이생물화학법검측뇨소、기항（creatinine， Cr）수평，대검측결과진행통계학분석。결과뇌사망후IL-8검측결과개시승고，3 h、6 h、9 h적IL-18검측결과균고우뇌사망전，차차이균유통계학의의（P균＜0.05）。뇌사망후각시간점IL-8적검측결과차이무통계학의의（H＝7.840，P＝0.098）。뇌사망후3 h、6 h、9 h적뇨소검측결과균고우뇌사망전，차이균유통계학의의（P균＜0.05）。뇌사망후6 h、9 h적Cr검측결과균고우뇌사망전，차이균유통계학의의（P균＜0.05）。뇌사망전후혈청IL-8여뇨소검측결과정정상관성（r＝0.453，P＝0.012），여Cr검측결과무상관성（r＝0.209，P＝0.267）。결론뇌사망가인기조직석방IL-8，병가조성조직세포급신공능불가역성손상。인차，림상위보호잠재이식기관응재뇌사망학립이후급시채취유효적혈액정화흡부IL-8，이강저조직기관적염성손상。
Objective To study the change of interleukin-8 (IL-8) and its correlation with renal function index in brain dead organ donor model. Methods 7 heads of standard experimental Changbai pigs were used. Blood samples were taken after operation preparation and at immediately time as well as 3 h , 6 h, 9 h after brain death which caused by method of epidural intracranial pressure. Serum IL-8 concentration was determined by immune chip fluorescent quantitative method and the corresponding kidney function indexes were detected by biochemistry methods. The results were analyzed statistically. Results Serum IL-8 concen-tration increased gradually after brain death. The concentration of IL-8 at 3 h, 6 h, 9 h after brain death were all higher than that of before brain death, and the differences all had statistical significance (Pall< 0.05). There was no statistical significance in the differences of IL-8 concentration among different time after brain death (H=7.840, P=0.098). The urea levels at 3 h, 6 h, 9 h after brain death were all higher than that of be-fore brain death, and the differences all had statistical significance (Pall<0.05). The creatinine (Cr) levels at 6h, 9h after brain death were all higher than that of before brain death, and the differences all had statistical significance(Pall<0.05). The concentration of IL-8 was positively correlated with urea(r=0.453, P=0.012), but not correlated with Cr (r=0.209, P=0.267). Conclusion Brain death induce plenty of IL-8 released, and which can cause irreversible damage to the tissue cells and renal function. Therefore , the clinical measures should be taken as soon as possible after brain death in order to reduce the organ inflammatory damage of dona-tion after brain death.
