CAJ | 학술논문

以白叶1号为试验材料，通过RT-PCR和RACE技术克隆获得茶树磷酸烯醇式丙酮酸转运子基因CsPPT （GenBank登录号：KJ652972）。CsPPT完整 ORF长度为1227 bp，编码408个氨基酸，蛋白分子量为44.7 kDa，理论等电点为10.16；无信号肽位点，属于非分泌型蛋白；建立了茶树 CsPPT蛋白的系统发育树；磷酸化修饰预测该蛋白质多肽链中共有26个磷酸化位点；TMHMM预测表明CsPPT蛋白为跨膜蛋白；亚细胞定位发现，CsPPT蛋白定位于叶绿体上，推测CsPPT蛋白可能定位于叶绿体膜上。荧光定量PCR结果表明CsPPT基因在茶树花中表达量最高，其次为芽、叶和嫩茎，根中最低。
이백협1호위시험재료，통과RT-PCR화RACE기술극륭획득다수린산희순식병동산전운자기인CsPPT （GenBank등록호：KJ652972）。CsPPT완정 ORF장도위1227 bp，편마408개안기산，단백분자량위44.7 kDa，이론등전점위10.16；무신호태위점，속우비분비형단백；건립료다수 CsPPT단백적계통발육수；린산화수식예측해단백질다태련중공유26개린산화위점；TMHMM예측표명CsPPT단백위과막단백；아세포정위발현，CsPPT단백정위우협록체상，추측CsPPT단백가능정위우협록체막상。형광정량PCR결과표명CsPPT기인재다수화중표체량최고，기차위아、협화눈경，근중최저。
The open reading frame (ORF) ofCsPPT(GenBank accession number: KJ652972)fromCamellia sinensis‘Baiye1’was obtained using RT-PCR and RACE technology.ThededucedORF ofCsPPThas1227 nucleotides, encoding 408 amino acids. Bioinformatic analysis showed that the molecular weight of the predicted protein was 44.7 kDa,and the theoretic isoelectric point was 10.16.The predicted protein had no signal peptide, belonging to non-secretory protein. Poylogenetic treeofCsPPThadbeenbuilt. Phosphorylation predictionshowedthe whole protein polypeptidecontained26 phosphorylation sites.Subcellular localization ofCsPPTprotein displayed that it waslocatedinthe chloroplast. TMHMM analysisshowedthatCsPPTproteinbelongedtotransmembrane proteins. QRT-PCR analysis results showed that the expression ofCsPPThas obvious tissue specificity, which was higher in flower,followed by buds, leaves, and tender stems,but lower in roots.