临床合理用药杂志
臨床閤理用藥雜誌
림상합리용약잡지
Chinese Journal of Clinical Rational Drug Use
2015年
29期
24-26
,共3页
婴幼儿%咽拭子%呼吸道病毒%实时荧光PCR技术
嬰幼兒%嚥拭子%呼吸道病毒%實時熒光PCR技術
영유인%인식자%호흡도병독%실시형광PCR기술
Infant%Throat swab%Respirovirus%RT-PCR
目的:了解衡阳地区春夏两季婴幼儿上呼吸道病毒感染的流行状况。方法收集2013年2~8月门诊和住院患儿咽拭子855例,其中春季(2~5月)468例,夏季(6~8月)387例;分别采用荧光抗体检测副流感病毒1、副流感病毒2、副流感病毒3,流感病毒A、流感病毒B、呼吸道合胞病毒、腺病毒,采用实时荧光PCR定量(RT-PCR)检测EB病毒。结果病毒检出率35.8%(306/855),副流感病毒1春季1.3%(6/468),夏季6.7%(26/387),差异有显著性(χ2=17.4,P<0.01);副流感病毒2春季0.65%(3/468),夏季2.2%(9/387),差异无显著性(χ2=4.3,P>0.05);副流感病毒3春季1.3%(6/468),夏季4.6%(18/387),差异有统计学意义(χ2=8.8,P<0.05);流感病毒( A、B)春季均为0.65%(3/468),夏季均未检出,差异无统计学意义(χ2=2.5,P>0.05);呼吸道合胞病毒( RSV),春季2.2%(10/458),夏季0.3%(1/386)(χ2=5.9,P <0.05);腺病毒(ADV),春季5.1%(24/468),夏季13.2%(51/387),差异有统计学意义(χ2=17.1,P<0.01);EB病毒,春季16.9%(79/468),夏季17.3%(67/387),差异无统计学意义(χ2=0.02,P>0.05);同时检出腺病毒和EB病毒共57例检出率为6.7%(57/855)。结论 EB病毒、腺病毒、副流感病毒(1、2、3)型是衡阳地区患儿上呼吸道感染的主要病原体,除EB病毒外其他病毒流行有一定季节性。
目的:瞭解衡暘地區春夏兩季嬰幼兒上呼吸道病毒感染的流行狀況。方法收集2013年2~8月門診和住院患兒嚥拭子855例,其中春季(2~5月)468例,夏季(6~8月)387例;分彆採用熒光抗體檢測副流感病毒1、副流感病毒2、副流感病毒3,流感病毒A、流感病毒B、呼吸道閤胞病毒、腺病毒,採用實時熒光PCR定量(RT-PCR)檢測EB病毒。結果病毒檢齣率35.8%(306/855),副流感病毒1春季1.3%(6/468),夏季6.7%(26/387),差異有顯著性(χ2=17.4,P<0.01);副流感病毒2春季0.65%(3/468),夏季2.2%(9/387),差異無顯著性(χ2=4.3,P>0.05);副流感病毒3春季1.3%(6/468),夏季4.6%(18/387),差異有統計學意義(χ2=8.8,P<0.05);流感病毒( A、B)春季均為0.65%(3/468),夏季均未檢齣,差異無統計學意義(χ2=2.5,P>0.05);呼吸道閤胞病毒( RSV),春季2.2%(10/458),夏季0.3%(1/386)(χ2=5.9,P <0.05);腺病毒(ADV),春季5.1%(24/468),夏季13.2%(51/387),差異有統計學意義(χ2=17.1,P<0.01);EB病毒,春季16.9%(79/468),夏季17.3%(67/387),差異無統計學意義(χ2=0.02,P>0.05);同時檢齣腺病毒和EB病毒共57例檢齣率為6.7%(57/855)。結論 EB病毒、腺病毒、副流感病毒(1、2、3)型是衡暘地區患兒上呼吸道感染的主要病原體,除EB病毒外其他病毒流行有一定季節性。
목적:료해형양지구춘하량계영유인상호흡도병독감염적류행상황。방법수집2013년2~8월문진화주원환인인식자855례,기중춘계(2~5월)468례,하계(6~8월)387례;분별채용형광항체검측부류감병독1、부류감병독2、부류감병독3,류감병독A、류감병독B、호흡도합포병독、선병독,채용실시형광PCR정량(RT-PCR)검측EB병독。결과병독검출솔35.8%(306/855),부류감병독1춘계1.3%(6/468),하계6.7%(26/387),차이유현저성(χ2=17.4,P<0.01);부류감병독2춘계0.65%(3/468),하계2.2%(9/387),차이무현저성(χ2=4.3,P>0.05);부류감병독3춘계1.3%(6/468),하계4.6%(18/387),차이유통계학의의(χ2=8.8,P<0.05);류감병독( A、B)춘계균위0.65%(3/468),하계균미검출,차이무통계학의의(χ2=2.5,P>0.05);호흡도합포병독( RSV),춘계2.2%(10/458),하계0.3%(1/386)(χ2=5.9,P <0.05);선병독(ADV),춘계5.1%(24/468),하계13.2%(51/387),차이유통계학의의(χ2=17.1,P<0.01);EB병독,춘계16.9%(79/468),하계17.3%(67/387),차이무통계학의의(χ2=0.02,P>0.05);동시검출선병독화EB병독공57례검출솔위6.7%(57/855)。결론 EB병독、선병독、부류감병독(1、2、3)형시형양지구환인상호흡도감염적주요병원체,제EB병독외기타병독류행유일정계절성。
Objective To investigation of respiratory tract infections in infants and young children during spring and summer in Hengyang. Methods 855 samples were collected from nasopharyngeal swabs of infants of clinic service and hospi-talization in our hospital during 2~8 month,2013. And 468 from Spring(2~5 month),387 from Summer(6~8 month). The virus were detected by using direct fluorescent antibody(DFA);Parainfluenza Virus 1,2 and 3,Influenza Virus A and B, Respiratory Syncytial Virus,Adenovirus,EBV was determined by RT-PCR. Results The virus were observed in 306 out of 855 cases(35. 8%),the rates of between Spring and Summer are 28. 6%,44. 4%,respectively(P<0. 05). And the incidence is 1. 3%,0. 65%,1. 3%,0. 65%,0. 65%,5. 1%,2. 1%,16. 9% and 6. 7%,2. 2%,4. 6%,0%,0%,13. 2%,0. 3% 17. 3%among Parainflu 1,2 and 3,influenza A and B,ADV,RSV,EBV between Spring and Summer in Hengyang city,respectively. There are significantly different(P<0. 05)for Parainflu 1 and 3,ADV and RSV in Spring and Summer and others are not sig-nificantly different(P>0. 05). The co-infection of ADV and EBV was found in 57 out of 855 cases(6. 7%). Conclusion The predominant prevalence of respiratory virus are EBV,ADV and Parainfluenza Virus(1,2,3)for infants of upper respirato-ry tract infection of virus in Hengyang city in China. There are seasonal differencing for those virus but for EBV.