CAJ | 학술논문

目的：对比研究猫扣带回前部内脏伤害感受神经元与躯体伤害感受神经元膜电学特性，从膜电学方面为阐明内脏痛与躯体痛具有不同感受特性的机制提供实验依据。方法选择成龄猫77只，体质量2.0~3.5 kg，雄雌不限。根据在体微电极记录的扣带回前部神经元对电刺激内脏大神经或隐神经产生的诱发反应的特点及吗啡对该诱发反应的影响，检出具有长潜伏期（≥50 ms）的内脏伤害感受神经元或躯体伤害感受神经元。应用在体微电极向内脏伤害感受神经元或躯体伤害感受神经元内注入波宽50 ms、不同强度（-5 nA~+5 nA）的一系列极化电流，记录神经元膜电学反应，计算膜电学参数。结果在扣带回前部记录了254个内脏伤害感受神经元和172个躯体伤害感受神经元。内脏伤害感受神经元的内脏大神经诱发反应阈值比躯体伤害感受神经元的隐神经诱发反应阈值高；与躯体伤害感受神经元相比，内脏伤害感受神经元的膜电阻、膜电容、时间常数较大。结论扣带回前部内脏伤害感受神经元与躯体伤害感受神经元的膜电学特性存在差异，可能是内脏痛与躯体痛具有不同感受特性的膜电学基础。
목적：대비연구묘구대회전부내장상해감수신경원여구체상해감수신경원막전학특성，종막전학방면위천명내장통여구체통구유불동감수특성적궤제제공실험의거。방법선택성령묘77지，체질량2.0~3.5 kg，웅자불한。근거재체미전겁기록적구대회전부신경원대전자격내장대신경혹은신경산생적유발반응적특점급마배대해유발반응적영향，검출구유장잠복기（≥50 ms）적내장상해감수신경원혹구체상해감수신경원。응용재체미전겁향내장상해감수신경원혹구체상해감수신경원내주입파관50 ms、불동강도（-5 nA~+5 nA）적일계렬겁화전류，기록신경원막전학반응，계산막전학삼수。결과재구대회전부기록료254개내장상해감수신경원화172개구체상해감수신경원。내장상해감수신경원적내장대신경유발반응역치비구체상해감수신경원적은신경유발반응역치고；여구체상해감수신경원상비，내장상해감수신경원적막전조、막전용、시간상수교대。결론구대회전부내장상해감수신경원여구체상해감수신경원적막전학특성존재차이，가능시내장통여구체통구유불동감수특성적막전학기출。
Objective To perform a comparative study on membrane electrical properties of visceral and somatic nociceptive neurons of anterior cin?gulate gyrus(ACG)in cats,so as to provide the experimental basis for elucidating the mechanism of differences in perceptual qualities between vis?ceral pain and somatic pain from the membrane electrical aspects. Methods A total of 77 adult cats,female or male,weighting 2.0 to 3.5 kg were selected for the study. According to the properties of the greater splanchnic nerve(GSN)or saphenous nerve(SN)evoked responses of neurons in ACG and effect of morphine on the evoked responses,visceral nociceptive neurons(VNNs)having the long latency(≥50 ms)GSN evoked re?sponses or somatic nociceptive neurons(SNNs)having the long latency(≥50 ms)SN evoked responses were detected. With a glass microelectrode in vivo,a series of polarizing current of different intensity from-5 nA to+5 nA with a 50 ms duration were injected to these neurons in ACG,and the membrane electrical responses of these neurons were recorded. Finally,the membrane electrical parameters of these neurons were calculated. Re?sults Totally 254 VNNs and 172 SNNs were recorded in ACG. GSN evoked response threshold of VNNs were higher than SN evoked response threshold of SNNs. Compared with SNNs,the membrane resistance,the membrane capacity and the time constant of VNNs were larger. Conclusion Our data proved that there are some differences in the membrane electrical properties between VNNs and SNNs in ACG,which might be the mem?brane electrical basis for differences in perceptual qualities between visceral pain and somatic pain.