CAJ | 학술논문

目的：探索更年期高血压燥证大鼠模型的建立方法。方法2014年1—3月选取雌性自发性高血压大鼠（ SHR）12只，采用摸球法将SHR随机分为更年期高血压组和更年期高血压燥证造模组，每组6只。采用外科手术法摘除两组大鼠双侧卵巢，制备更年期高血压大鼠。对更年期高血压燥证造模组大鼠施加人工燥证环境，饲喂西北燥证食物进行造模。观察大鼠生物学特征（毛色、精神状态、兴奋度、进食量、饮水量、体质量、大小便性状、舌象、爪象等）、血清神经递质〔促肾上腺皮质激素释放激素（ CRH）、促肾上腺皮质激素（ ACTH）、去甲肾上腺素（ NE）、多巴胺（DA）、5－羟色胺（5－HT）〕及激素〔雌二醇（E2）、睾酮（T）〕水平和脏器（肝脏、脾脏、肾上腺、下丘脑）组织结构。结果更年期高血压燥证造模组大鼠较更年期高血压组体质量降低，饮水量增加，尿量减少（P＜0.05）。更年期高血压燥证造模组大鼠血清CRH、 NE、 DA、5－HT水平较更年期高血压组升高（P＜0.05）。两组大鼠ACTH水平比较，差异无统计学意义（ P＞0.05）。更年期高血压燥证造模组大鼠较更年期高血压组血清E2、 T水平降低， E2／T升高（P＜0.05）。两组大鼠肝脏、脾脏、肾上腺、下丘脑组织结构均发生明显改变。结论通过对更年期高血压大鼠施加人工燥证环境，给予西北燥证食物，控制饮水量及进食量，可成功建立更年期高血压燥证大鼠模型。
목적：탐색경년기고혈압조증대서모형적건립방법。방법2014년1—3월선취자성자발성고혈압대서（ SHR）12지，채용모구법장SHR수궤분위경년기고혈압조화경년기고혈압조증조모조，매조6지。채용외과수술법적제량조대서쌍측란소，제비경년기고혈압대서。대경년기고혈압조증조모조대서시가인공조증배경，사위서북조증식물진행조모。관찰대서생물학특정（모색、정신상태、흥강도、진식량、음수량、체질량、대소편성상、설상、조상등）、혈청신경체질〔촉신상선피질격소석방격소（ CRH）、촉신상선피질격소（ ACTH）、거갑신상선소（ NE）、다파알（DA）、5－간색알（5－HT）〕급격소〔자이순（E2）、고동（T）〕수평화장기（간장、비장、신상선、하구뇌）조직결구。결과경년기고혈압조증조모조대서교경년기고혈압조체질량강저，음수량증가，뇨량감소（P＜0.05）。경년기고혈압조증조모조대서혈청CRH、 NE、 DA、5－HT수평교경년기고혈압조승고（P＜0.05）。량조대서ACTH수평비교，차이무통계학의의（ P＞0.05）。경년기고혈압조증조모조대서교경년기고혈압조혈청E2、 T수평강저， E2／T승고（P＜0.05）。량조대서간장、비장、신상선、하구뇌조직결구균발생명현개변。결론통과대경년기고혈압대서시가인공조증배경，급여서북조증식물，공제음수량급진식량，가성공건립경년기고혈압조증대서모형。
Objective To explore the method of the establishment of climacteric hypertension dryness syndrome model in rats.Methods A total of 12 female spontaneously hypertensive rats ( SHR) were selected as study subjects from January to March in 2014, SHR were randomly divided into climacteric hypertension group and climacteric hypertension dryness syndrome model group , there were 6 rats in each group.The bilateral ovaries of rats were removed by surgery , thus climacteric hypertension rats were obtained.Rats in climacteric hypertension dryness syndrome model group were placed in artificial dryness syndrome environment, and were fed with food from northwest area which may cause dryness syndrome.The biological characteristics (coat color, mental state, degrees of excitement, food intake, water intake, body mass, characters of urine and stool, tongue picture, claws picture), serum levels of neurotransmitters 〔corticotropin -releasing hormone ( CRH), adrenocorticotrophic hormone (ACTH), norepinephrine (NE), dopamine (DA), 5 -hydroxytryptamine (5 -HT)〕 and hormones 〔estradiol (E2), testosterone ( T)〕, and histological changes of organs ( liver, spleen, adrenal gland, and hypothalamus ) were observed and recorded.Results Compared with rats in climacteric hypertension group , the body mass and urine volume were significantly decreased , and water intake was significantly increased among rats in climacteric hypertension dryness syndrome model group, the differences were statistically significant ( P <0.05 ) .Serum levels of CRH, NE, DA and 5 -HT in climacteric hypertension dryness syndrome model group were significantly higher than those in climacteric hypertension group ( P<0.05) .There was no significant difference in ACTH level between two groups ( P>0.05) .Compared with rats in climacteric hypertension group, serum levels of E2 and T were significantly decreased , and E2/T was significantly increased among rats in climacteric hypertension dryness syndrome model group , the differences were statistically significant (P<0.05) .The significant histological changes of liver , spleen, adrenal gland, and hypothalamus were observed among two groups of rats.Conclusion The artificial dryness syndrome environment , food from northwest area which may cause dryness syndrome , combined with control of food and water intake can successfully turn climacteric hypertension rats into climacteric hypertension dryness syndrome rats model.