科技通报
科技通報
과기통보
Bulletin of Science and Technology
2015年
9期
85-88
,共4页
鲁宇文%彭杰军%郑红英%林林%燕飞%陈剑平
魯宇文%彭傑軍%鄭紅英%林林%燕飛%陳劍平
로우문%팽걸군%정홍영%림림%연비%진검평
辣椒斑驳病毒%辣椒轻斑驳病毒%复合侵染%序列分析
辣椒斑駁病毒%辣椒輕斑駁病毒%複閤侵染%序列分析
랄초반박병독%랄초경반박병독%복합침염%서렬분석
pepper mottle virus%pepper mild mottle virus%mixed infection%sequence analysis
上海奉贤区发生的线椒病害其症状表现为斑驳、花叶，且果实扭曲畸形，严重影响了辣椒的产量和品质。通过电镜病毒粒子观察和RT-PCR检测，确定其毒源为辣椒斑驳病毒（pepper mottle virus， PepMoV）和辣椒轻斑驳病毒（pepper mild mottle virus，PMMoV）复合侵染。根据它们的外壳蛋白（coat protein，CP）核苷酸序列，在GenBank上进行BLAST比对，其同源性PepMoV为85%~99%，PMMoV为94%~99%。系统进化分析表明，PepMoV和PMMoV上海分离物变异均存在一定的地域相关性。这是首次在我国辣椒田间病毒病样中检测到这两种病毒的复合侵染。
上海奉賢區髮生的線椒病害其癥狀錶現為斑駁、花葉，且果實扭麯畸形，嚴重影響瞭辣椒的產量和品質。通過電鏡病毒粒子觀察和RT-PCR檢測，確定其毒源為辣椒斑駁病毒（pepper mottle virus， PepMoV）和辣椒輕斑駁病毒（pepper mild mottle virus，PMMoV）複閤侵染。根據它們的外殼蛋白（coat protein，CP）覈苷痠序列，在GenBank上進行BLAST比對，其同源性PepMoV為85%~99%，PMMoV為94%~99%。繫統進化分析錶明，PepMoV和PMMoV上海分離物變異均存在一定的地域相關性。這是首次在我國辣椒田間病毒病樣中檢測到這兩種病毒的複閤侵染。
상해봉현구발생적선초병해기증상표현위반박、화협，차과실뉴곡기형，엄중영향료랄초적산량화품질。통과전경병독입자관찰화RT-PCR검측，학정기독원위랄초반박병독（pepper mottle virus， PepMoV）화랄초경반박병독（pepper mild mottle virus，PMMoV）복합침염。근거타문적외각단백（coat protein，CP）핵감산서렬，재GenBank상진행BLAST비대，기동원성PepMoV위85%~99%，PMMoV위94%~99%。계통진화분석표명，PepMoV화PMMoV상해분리물변이균존재일정적지역상관성。저시수차재아국랄초전간병독병양중검측도저량충병독적복합침염。
Peppers from Fengxian district in Shanghai showed symptoms of mottle, mosaic on leaves and distortion on fruits, which greatly reduced the yield and quality of pepper. The results of electronic microscope observation and RT-PCR indicated the mixed infection of pepper mottle virus with pepper mild mottle virus. Compared their complete coat protein nucleotides equences on GenBank suggested the homology of PepMoV-SH is about 85%~99% and 94%~99% to PMMoV-SH. Phylogenetic analysis showed the evolutions of PMMoV-SH and PepMoV-SH are related to their regional distribution. This is the first report in China about mixed infection of Pepper mild mottle virus with Pepper mottle virus in field.
