CAJ | 학술논문

目的：本研究拟采用病例对照方法探讨人类白细胞抗原（HLA ）‐DRB1*基因单核苷酸多态性与新疆地区维吾尔族及汉族溃疡性结肠炎（UC）易感性的关联性，并应用5‐氨基水杨酸（5‐ASA ）治疗，观察治疗反应，探讨HLA‐DRB1*基因单核苷酸多态性与UC疗效之间的关系。方法采用序列特异性引物聚合酶链反应的方法及SBT测序法对120例UC患者和408名健康对照者DNA的HLA‐DRB1基因分型，病例组中汉族66例，维吾尔族54例，男性57例，女性63例，平均发病年龄为（48．09±15．63）岁；健康对照组408例，其中汉族212例，维吾尔族196例。120例患者均口服5‐A S A ，急性期4 g／d ，缓解期2 g／d ，随餐服用，随访6个月后观察疗效，根据疗效分为治疗有效组及治疗无效组。结果汉族UC患者HLA‐DRB1*13等位基因频率高于健康对照组，差异有统计学意义（P＜0．05）；HLA‐DRB1*12等位基因频率低于健康对照组，差异有统计学意义（ P＜0．05）；维吾尔族U C 患者H L A‐D RB1*01、*04、*13、*14等位基因频率高于健康对照组，差异有统计学意义（P＜0．05）。120例 UC 患者口服5‐ASA 后对疗效进行比较，发现H L A‐D RB1*04、*13、*14等位基因阳性的维吾尔族与汉族U C患者相比较，两组间的治疗效果差异无统计学意义（P＞0．05）。结论 HLA‐DRB1*基因单核苷酸多态性与新疆地区汉族UC无明显关系，与维吾尔族UC的易感性有关，它们可能在 UC 的发病中发挥潜在作用，但与新疆地区维吾尔族 UC患者服用5‐ASA的治疗效果无明显关系。
목적：본연구의채용병례대조방법탐토인류백세포항원（HLA ）‐DRB1*기인단핵감산다태성여신강지구유오이족급한족궤양성결장염（UC）역감성적관련성，병응용5‐안기수양산（5‐ASA ）치료，관찰치료반응，탐토HLA‐DRB1*기인단핵감산다태성여UC료효지간적관계。방법채용서렬특이성인물취합매련반응적방법급SBT측서법대120례UC환자화408명건강대조자DNA적HLA‐DRB1기인분형，병례조중한족66례，유오이족54례，남성57례，녀성63례，평균발병년령위（48．09±15．63）세；건강대조조408례，기중한족212례，유오이족196례。120례환자균구복5‐A S A ，급성기4 g／d ，완해기2 g／d ，수찬복용，수방6개월후관찰료효，근거료효분위치료유효조급치료무효조。결과한족UC환자HLA‐DRB1*13등위기인빈솔고우건강대조조，차이유통계학의의（P＜0．05）；HLA‐DRB1*12등위기인빈솔저우건강대조조，차이유통계학의의（ P＜0．05）；유오이족U C 환자H L A‐D RB1*01、*04、*13、*14등위기인빈솔고우건강대조조，차이유통계학의의（P＜0．05）。120례 UC 환자구복5‐ASA 후대료효진행비교，발현H L A‐D RB1*04、*13、*14등위기인양성적유오이족여한족U C환자상비교，량조간적치료효과차이무통계학의의（P＞0．05）。결론 HLA‐DRB1*기인단핵감산다태성여신강지구한족UC무명현관계，여유오이족UC적역감성유관，타문가능재 UC 적발병중발휘잠재작용，단여신강지구유오이족 UC환자복용5‐ASA적치료효과무명현관계。
Objective This paper was to explore the relationship of HLA‐DRB1 * gene polymorphism between Uyghur and Han UC patients in Xinjiang Uygur Autonomous Region and the treatment response using 5‐amino salicylic acid ,and to explore the relationship between human leukocyte antigen‐DRB1 * polymorphisms and treatment response of ulcerative colitis . Methods The specific sequence primer polymerase chain reaction method and SBT sequencing method were used for HLA‐DRB1* genotyping of 120 cases of UC patients and 408 cases of normal controls .The 66 cases were Han and 54 cases were Uyghur in the treatment group ,with an average onset age of (48 .09 ± 15 .63) years .The 212 cases were Han and 196 cases were Uyghur in the control group .120 patients took 5‐amino salicylic acid preparations orally ,with a dosage of 4 g/d in the acute phase and 2 g/d in the remission phase ,with meals and after 6 months of follow‐up efficacy .Results The HLA‐DRB 1*13 allele frequency of Han UC patients is higher than that in the control group ,with statistical significant difference(P<0 .05) .The HLA‐DRB1* 12 allele frequency of Han UC patients is lower than that in the control group ,with statistical significant difference (P<0 .05) .The HLA‐DRB*01 ,*04 ,*13 ,*14 allele frequency of Uyghur patients is higher than that of the control group ,with statistical significant difference (P< 0 .05) .However ,no significant difference was found between the Han and Uyghur UC patients ,after taking 5‐amino salicylic acid preparations orally .Conclusions Human leukocyte antigen‐DRB1 * gene polymorphism is associated with Uyghur and ulcerative colitis susceptibility .It may play a potential role in the pathogenesis of UC in the Uyghur in Xinjiang Autonomous Region . However , there is no significant difference ,after taking 5‐amino salicylic acid preparations orally between the Han and Uyghur UC patients .