CAJ | 학술논문

3.85 kg的孔石莼干粉末用甲醇提取得到320 g粗提物浸膏，浸膏被萃取分离为石油醚相、乙酸乙酯相、正丁醇相和蒸馏水相。对这4个相进行了高效液相色谱（HPLC）分析，然后对石油醚相和乙酸乙酯相进行了气相色谱－质谱（GC/MS）联用分析。用赤潮异弯藻进行各个分离组分的杀藻活性检测。活性检测表明石油醚相和乙酸乙酯相具有很强的杀藻活性，GC/MS分析鉴定其中的16种脂肪酸，其中不饱和脂肪酸占大部分。活性检测表明9,12,15-十八碳三烯酸、6，9，12，15-十八碳四烯酸、5Z，8Z，11Z，14Z-二十碳四烯酸、5Z，11Z，14Z，17Z-二十碳四烯酸和5Z，8Z，11Z，14Z，17Z-二十碳五烯酸具有很强的杀藻活性，其有效浓度在5 mg L-1以下。实验结果表明，孔石莼组织中含有多种不饱和脂肪酸，其中一些对赤潮异弯藻具有强烈的杀藻作用，不饱和脂肪酸可能是孔石莼组织内抑制赤潮微藻生长的他感物质重要组成成分之一。
3.85 kg적공석순간분말용갑순제취득도320 g조제물침고，침고피췌취분리위석유미상、을산을지상、정정순상화증류수상。대저4개상진행료고효액상색보（HPLC）분석，연후대석유미상화을산을지상진행료기상색보－질보（GC/MS）련용분석。용적조이만조진행각개분리조분적살조활성검측。활성검측표명석유미상화을산을지상구유흔강적살조활성，GC/MS분석감정기중적16충지방산，기중불포화지방산점대부분。활성검측표명9,12,15-십팔탄삼희산、6，9，12，15-십팔탄사희산、5Z，8Z，11Z，14Z-이십탄사희산、5Z，11Z，14Z，17Z-이십탄사희산화5Z，8Z，11Z，14Z，17Z-이십탄오희산구유흔강적살조활성，기유효농도재5 mg L-1이하。실험결과표명，공석순조직중함유다충불포화지방산，기중일사대적조이만조구유강렬적살조작용，불포화지방산가능시공석순조직내억제적조미조생장적타감물질중요조성성분지일。
3.85 kg dry powder of Ulva pertusa was extracted with methanol, and 320 g extracts was obtained. The extracts were partitioned to petroleum ether phase, ethyl acetate phase, butanol phase and distilled water phase by liquid-liquid fractionation. Thereafter, the 4 phases were analyzed by HPLC, and petroleum ether phase and ethyl acetate phase were analyzed by GC/MS. Bioassays of the activity of every fraction were carried out on Heterosigma akashiwo. Petroleum ether phase and ethyl acetate phase had strong algicidal effect on the microalga. The results of GC/MS revealed that there were 16 fatty acids in the 2 phases, and most of them were unsaturated fatty acids. The results of bioassays demonstrated that 5 unsaturated fatty acids in the tissue of Ulva pertusa had strong algicidal effect on Heterosigma akashiwo, and the effective concentrations were below 5 mg L-1. The results of this study demonstrated that unsaturated fatty acids in the tissue of Ulva pertusa might be one of the components of the allelochemicals in the macroalgae.