CAJ | 학술논문

目的：观察萝卜硫素对胃癌和胰腺癌细胞活力、侵袭能力、周期、凋亡、DNA片段和相关蛋白的影响，为日常饮食提供参考，为临床治疗胃癌和胰腺癌提供实验数据。方法：通过CCK-8和transwell侵袭实验分析初步判断萝卜硫素对SGC-7901胃癌细胞和PANC-1胰腺癌细胞活性和转移侵袭的影响，计算体外干预SGC-7901和PANC-1的IC50，流式细胞学分析IC50浓度萝卜硫素对细胞周期和凋亡的影响，电泳分析DNA片段化改变。免疫印迹与免疫组化实验观察炎症和转移相关蛋白水平变化。结果：CCK-8结果显示SGC-7901和PANC-1细胞活性对萝卜硫素剂量依赖性下降，萝卜硫素作用于SGC-7901和PANC-1细胞的IC50分别为4.5μg/mL和5.5μg/mL（24 h），该浓度的萝卜硫素抑制了SGC-7901和PANC-1细胞的转移侵袭，阻滞细胞于G0/G1期，诱导细胞凋亡并使DNA发生片段化变化，下调TNF-α、TGF-β和NF-κB的表达。结论：萝卜硫素可以抑制胃癌和胰腺癌细胞的生长和侵袭，抑制炎症蛋白的表达，是胃癌和胰腺癌辅助治疗的新途径。
목적：관찰라복류소대위암화이선암세포활력、침습능력、주기、조망、DNA편단화상관단백적영향，위일상음식제공삼고，위림상치료위암화이선암제공실험수거。방법：통과CCK-8화transwell침습실험분석초보판단라복류소대SGC-7901위암세포화PANC-1이선암세포활성화전이침습적영향，계산체외간예SGC-7901화PANC-1적IC50，류식세포학분석IC50농도라복류소대세포주기화조망적영향，전영분석DNA편단화개변。면역인적여면역조화실험관찰염증화전이상관단백수평변화。결과：CCK-8결과현시SGC-7901화PANC-1세포활성대라복류소제량의뢰성하강，라복류소작용우SGC-7901화PANC-1세포적IC50분별위4.5μg/mL화5.5μg/mL（24 h），해농도적라복류소억제료SGC-7901화PANC-1세포적전이침습，조체세포우G0/G1기，유도세포조망병사DNA발생편단화변화，하조TNF-α、TGF-β화NF-κB적표체。결론：라복류소가이억제위암화이선암세포적생장화침습，억제염증단백적표체，시위암화이선암보조치료적신도경。
Objective:To investigate the effect of vitality, invasion, cell cycle, apoptosis, DNA fragment and relative proteins expression in gastric cancer SGC-7901 and pancreatic cancer PANC-1 cell line by sulforaphane(SFN)derived from broccoli, to provide reference for daily diet and supply experiment data for treatment gastric cancer and pancreatic cancer. Methods:Analyzed vitality and invasion of SGC-7901 and PANC-1 cells treated with sulforaphane by cell counting kit (CCK8) and transwell, then measure the half maximal (50%) inhibitory concentration (IC50) of sulforaphane for SGC-7901 and PANC-1 cells. The cells cycle, apoptosis and DNA fragment were analyzed using Flow Cytometry Analysis and agarose electrophoresis, TNF-α, TGF-β and NF-κB were analyzed by western blot and immunohistochemistry after treatment with sulforaphane. Results:Results showed that SGC-7901 and PANC-1 cells proliferate and invade were inhibited by sulforaphane with a dose-dependent manner, IC50 of sulforaphane was 4.5μg/mL(SGC-7901,24h) and 5.5μg/mL (PANC-1,24h), and the cell cycle were arrested at G0/G1 phase. 4.5μg/mL and 5.5μg/mL sulforaphane induced apoptosis, DNA fragment, decreased the expression of TNF-α, TGF-β and NF-κB in SGC-7901 and PANC-1 cells. Conclusion: Sulforaphane inhibited proliferation and invasion of gastric cancer SGC-7901 and pancreatic cancer PANC-1 cells in vitro, decreased the expression of inflammation proteins, maybe a novel chemotherapy for gastric cancer and pancreatic cancer.