CAJ | 학술논문

目的：观察组蛋白去乙酰化酶抑制药SAHA联合顺铂（cis-diamminedichloroplatinum，DDP）抑瘤作用及机制。方法饲养40只Balb/c-nu/nu雌性裸鼠，细胞悬液皮下注射法构建人宫颈癌SiHa细胞株裸鼠移植瘤模型，当移植瘤8~10 mm大小时，36只荷瘤裸鼠被随机分为6组（对照组、25 mg/kg SAHA组、50 mg/kg SAHA组、5 mg/kg DDP组、25 mg/kg SAHA+DDP组、50 mg/kg SAHA+DDP组），给药第17天拉颈处死裸鼠。对移植瘤的湿重、瘤体积、肿瘤生长抑制率输入SPSS 19.0软件进行统计学分析。结果与各对照组比，联合组显示出最大程度的肿瘤体积的缩小及肿瘤生长抑制率增加（P＜0.05）。结论 SAHA与DDP联合治疗宫颈癌，其作用机制可能与组蛋白去乙酰化酶1（histone deacetylase 1，HDAC1）蛋白水平下调有关。
목적：관찰조단백거을선화매억제약SAHA연합순박（cis-diamminedichloroplatinum，DDP）억류작용급궤제。방법사양40지Balb/c-nu/nu자성라서，세포현액피하주사법구건인궁경암SiHa세포주라서이식류모형，당이식류8~10 mm대소시，36지하류라서피수궤분위6조（대조조、25 mg/kg SAHA조、50 mg/kg SAHA조、5 mg/kg DDP조、25 mg/kg SAHA+DDP조、50 mg/kg SAHA+DDP조），급약제17천랍경처사라서。대이식류적습중、류체적、종류생장억제솔수입SPSS 19.0연건진행통계학분석。결과여각대조조비，연합조현시출최대정도적종류체적적축소급종류생장억제솔증가（P＜0.05）。결론 SAHA여DDP연합치료궁경암，기작용궤제가능여조단백거을선화매1（histone deacetylase 1，HDAC1）단백수평하조유관。
[Absrract]ObjectiveTo study the effect of histone deacetylase inhibitor SAHA (N-Hydroxy-N-phenyloctanediamide) combined with cis-diamminedichloroplatinum (DDP) on cervical cancer cells (SiHa) in nude mice.MethodsCervical cancer cell suspension was subcutaneously injected into 40 nude mice to establish cervical cancer model. When the transplanted tumor was about 8 mm in length, 36 mice with tumor in similar size were selected and divided into six groups (control group, 25 mg/kg SAHA group, 50 mg/kg SAHA group, 5 mg/kg DDP group, 25 mg/kg SAHA+DDP group) , and 50 mg/kg SAHA+DDP group. Nude mice were killed on the 17th day. Wet weight of tumor, inhibitory rate and tumor volume were collected. SPSS 19.0 software was used for statistical analysis.ResultsCompared with the control group, the DDP plus SAHA group showed superiority in inhibiting tumor growth (P<0.05).ConclusionThe effects from SAHA and DDP might be associated with down-regulating expressions of HDAC1 protein.