CAJ | 학술논문

目的：研究持续性高正加速度(+Gz)下三叉神经脊束核尾侧亚核（spinal trigeminal nucleus caudal subnucleus， Vc）内P物质（substance P，SP）与N-甲基-D-天冬氨酸受体（N-methyl-D-aspartate receptor antagonists，NMDAR），以及三叉神经节（trigeminal ganglia，TG）内前速激肽原A（preprotachykinin A，ppTA）、β-降钙素基因相关肽（β-calcition-gene-related peptide，β-CGRP）、PN3与NaN的蛋白表达的变化。方法采用随机法选取36只SD大鼠，分为对照组（N），+5 Gz组（5 G），+10 Gz组（10 G），将各组大鼠依次固定于实验用小动物离心机。在不同+Gz数值条件下行高+Gz模拟处理程序：在+5 Gz条件下（+5 Gz峰值持续作用时间为30 s，间隔峰值为+1 Gz、60 s）持续离心5 min，每天连续离心5次，每周4 d，持续3周；在+10 Gz条件下（+10 Gz峰值持续作用时间为30 s，间隔峰值+1 Gz、60 s）保持离心5 min，每天连续离心5次，每周4 d，持续3周；对照组大鼠依照上述同样作用时间和频次，只在离心机的相同固定装置上保持固定5 min，不做任何持续高+Gz离心实验干预。实验完成后即刻取大鼠三叉神经节与三叉神经脊束核尾侧亚核，提取总蛋白，Western-Blot检测SP、NMDAR、ppTA、β-CGRP、PN3与NaN的蛋白表达。结果和对照组比较，+5 Gz组、+10 Gz组TG与Vc内的SP、NMDAR、ppTA、β-CGRP、PN3与NaN的蛋白表达明显增高（P＜0.05）。结论说明在高压环境下，初级感觉神经元的生化特征发生了显著变化，这些神经活性物质在颞下颌关节疾病（temporomandibular disorder，TMD）引起的疼痛传导中发挥了非常重要的作用，表明高压环境下上述神经活性物质与颞颌关节疼痛的外周及中枢的传导作用机制密切相关。
목적：연구지속성고정가속도(+Gz)하삼차신경척속핵미측아핵（spinal trigeminal nucleus caudal subnucleus， Vc）내P물질（substance P，SP）여N-갑기-D-천동안산수체（N-methyl-D-aspartate receptor antagonists，NMDAR），이급삼차신경절（trigeminal ganglia，TG）내전속격태원A（preprotachykinin A，ppTA）、β-강개소기인상관태（β-calcition-gene-related peptide，β-CGRP）、PN3여NaN적단백표체적변화。방법채용수궤법선취36지SD대서，분위대조조（N），+5 Gz조（5 G），+10 Gz조（10 G），장각조대서의차고정우실험용소동물리심궤。재불동+Gz수치조건하행고+Gz모의처리정서：재+5 Gz조건하（+5 Gz봉치지속작용시간위30 s，간격봉치위+1 Gz、60 s）지속리심5 min，매천련속리심5차，매주4 d，지속3주；재+10 Gz조건하（+10 Gz봉치지속작용시간위30 s，간격봉치+1 Gz、60 s）보지리심5 min，매천련속리심5차，매주4 d，지속3주；대조조대서의조상술동양작용시간화빈차，지재리심궤적상동고정장치상보지고정5 min，불주임하지속고+Gz리심실험간예。실험완성후즉각취대서삼차신경절여삼차신경척속핵미측아핵，제취총단백，Western-Blot검측SP、NMDAR、ppTA、β-CGRP、PN3여NaN적단백표체。결과화대조조비교，+5 Gz조、+10 Gz조TG여Vc내적SP、NMDAR、ppTA、β-CGRP、PN3여NaN적단백표체명현증고（P＜0.05）。결논설명재고압배경하，초급감각신경원적생화특정발생료현저변화，저사신경활성물질재섭하합관절질병（temporomandibular disorder，TMD）인기적동통전도중발휘료비상중요적작용，표명고압배경하상술신경활성물질여섭합관절동통적외주급중추적전도작용궤제밀절상관。
ObjectiveTo investigate the possible role of substance P(SP), N-methyl-D-aspartate receptor antagonists(NMDAR)in rat spinal trigeminal nucleus caudal subnucleus(Vc) and ppTA,β-calcitonin-gene-related peptide(β-CGRP), PN3, NaN in the trigeminal ganglion(TG) under repeated +Gz condition.Methods 36 male SD rats were divided into control group(N), +5 Gz group(5 G), +10 Gz group(10 G) randomly. The rats in each group were fixed in the experiment with small animal centrifuge. Process simulation in different + Gz numerical conditions. +5 Gz borne +5 Gz group for 5 minutes(onset rats about 0.5 G/s, 5 times/d with +1 Gz for 1 minute, each for 30 s, with intervals 4 d/wk, 3 weeks in total). +10 Gz group was repeatedly exposed to condition +10 Gz(onset rats about 0.5 G/s, 5 times/d with +1 Gz for 1 minute intervals, each for 30 s, with intervals 4 d/wk, lasting 3 weeks in total). Control group rats were in accordance with the same time and frequency, only in the centrifuge the same fixed device remain fixed for 5 min, did not do any sustained +Gz centrifuge experiment intervention. TD and SpVc were removed after +Gz examination. After the total protein extracted, the expression of SP, NMDAR, ppTA,β-CGRP, PN3 and NaN were detected by Western-Blot analysis.ResultsCompared with the control group, the +5 Gz group and the +10 Gz group expressions of SP, NMDAR, ppTA,β-CGRP, PN3 and NaN were significantly up-regulated(P<0.05).ConclusionIn the +Gz environment, the biochemical characteristics of the primary sensory neurons were significantly changed, and the active substance in the TMD was very important in the pain transmission. These indicate that the above-mentioned neural active substances is closely related to the conduction mechanism of the peripheral and central nervous system in +Gz environment.