CAJ | 학술논문

目的：探讨microRNA129（miR129）在胃管状腺瘤、胃癌组织及其对应的癌旁组织中的表达及其临床意义。方法收集经高年资病理医师确诊的55例管状腺瘤、76例胃腺癌组织及对应的癌旁组织，分别用原位杂交方法检测miR129的表达，分析miR129表达与临床病理参数之间的关系。结果癌旁胃黏膜组织中miR-129的表达阳性率高于胃管状腺瘤组织，胃管状腺瘤组织中miR-129的表达阳性率高于胃腺癌组织（ P ＜0憫．05）。 miR129在高分化胃癌组织中阳性表达率高于中、低分化组织（ P ＜0．05）；临床分期Ⅰ期中的阳性表达率高于Ⅲ期；无淋巴结转移组织中的表达阳性率高于有淋巴结转移组织（ P ＜0．05）；胃管状腺癌中miR129阳性率高于弥漫型腺癌（ P ＜0．05）。结论miR129在胃腺癌组织内低表达，发挥着抑癌基因的作用；miR129可作为胃腺癌的判断预后的标志物。
목적：탐토microRNA129（miR129）재위관상선류、위암조직급기대응적암방조직중적표체급기림상의의。방법수집경고년자병리의사학진적55례관상선류、76례위선암조직급대응적암방조직，분별용원위잡교방법검측miR129적표체，분석miR129표체여림상병리삼수지간적관계。결과암방위점막조직중miR-129적표체양성솔고우위관상선류조직，위관상선류조직중miR-129적표체양성솔고우위선암조직（ P ＜0민．05）。 miR129재고분화위암조직중양성표체솔고우중、저분화조직（ P ＜0．05）；림상분기Ⅰ기중적양성표체솔고우Ⅲ기；무림파결전이조직중적표체양성솔고우유림파결전이조직（ P ＜0．05）；위관상선암중miR129양성솔고우미만형선암（ P ＜0．05）。결론miR129재위선암조직내저표체，발휘착억암기인적작용；miR129가작위위선암적판단예후적표지물。
Objective To investigate the expression and clinical significance of microRNA129 ( miR129 ) in gastric tubular adenoma tissues,gastric adenocarcinoma tissues and corresponding para-carcinoma tissues.Methods Fifty-five cases of gastric tubular adenoma, 76 cases of gastric adenocarcinoma and corresponding para-carcinoma tissues that were identified by senior pathology doctors were enrolled in the study.The expression levels of miR-129 were detected by in situ hybrization, and the correlation between miR129 expression and clinicopathological parameters was analyzed.Results The positive expression rate of miR-129 in para-carcinoma gastric mucosa tissues was significantly higher than that in gastric tubular adenoma tissues, moreover, which in gastric tubular adenoma tissues was significantly higher than that in gastric adenocarcinoma tissues ( P <0.05).The positive expression rate in well differentiated gastric cancer tissues was significantly higher than that in moderately differentiated and poorly differentiated tissues ( P <0.05).The positive expression rate of miR-129 in stageⅠcarcinoma tissues was obviously higher than that in stage Ⅲ carcinoma tissues, furthermore, which in carcinoma tissues without lymphonode metastasis was significantly higher than that in carcinoma tissues with lymphonode metastasis ( P <0.05).The positive expression rate of miR-129 in gastric tubular adenoma was significantly higher than that in diffuse type adenocarcinoma ( P <0.05).Conclusion The expression levels of miR129 are down-regulated in gastric adenocarcinoma,which may play a role of anti-oncogene, thus miR-129 may be regarded as a marker for evaluating the prognosis of patients with gastric adenocarcinoma.