牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
Chinese Journal of Conservative Dentistry
2015年
9期
524-528
,共5页
淫羊藿苷%牙髓干细胞%增殖%骨向分化
淫羊藿苷%牙髓榦細胞%增殖%骨嚮分化
음양곽감%아수간세포%증식%골향분화
icariin%dental pulp stem cells%proliferation%osteogenic differentiation
目的:探讨淫羊藿苷对人牙髓干细胞( hDPSCs)增殖及骨向分化的影响. 方法:MTT检测10 -2 ~10 -10 mol/L淫羊藿苷对hDPSCs增殖能力的影响,并筛选出最佳浓度(10 -5 mol/L);然后以10 -5 mol/L的淫羊藿苷作用于hDPSCs,并对其进行矿化诱导. 诱导培养21 d后,用Real-time PCR检测hDPSCs中ALP、DMP-1、DSPP各矿化相关基因的表达水平;茜素红染色观察hDPSCs矿化结节的能力. 结果:与对照组相比, 10 -2 ~10 -3 mol/L 的淫羊藿苷对hDPSCs增殖有一定的抑制作用(P<0. 05),而10 -5 mol/L和10 -6 mol/L的淫羊藿苷均能明显促进hDPSCs的增殖(P<0. 05),其中以10 -5 mol/L最好;Real-time PCR检测及茜素红染色观察结果显示,10 -5 mol/L淫羊藿苷可明显促进hDPSCs中各矿化相关基因的表达和矿化结节的形成. 结论: 一定浓度范围的淫羊藿苷可促进hDPSCs的增殖和骨向分化.
目的:探討淫羊藿苷對人牙髓榦細胞( hDPSCs)增殖及骨嚮分化的影響. 方法:MTT檢測10 -2 ~10 -10 mol/L淫羊藿苷對hDPSCs增殖能力的影響,併篩選齣最佳濃度(10 -5 mol/L);然後以10 -5 mol/L的淫羊藿苷作用于hDPSCs,併對其進行礦化誘導. 誘導培養21 d後,用Real-time PCR檢測hDPSCs中ALP、DMP-1、DSPP各礦化相關基因的錶達水平;茜素紅染色觀察hDPSCs礦化結節的能力. 結果:與對照組相比, 10 -2 ~10 -3 mol/L 的淫羊藿苷對hDPSCs增殖有一定的抑製作用(P<0. 05),而10 -5 mol/L和10 -6 mol/L的淫羊藿苷均能明顯促進hDPSCs的增殖(P<0. 05),其中以10 -5 mol/L最好;Real-time PCR檢測及茜素紅染色觀察結果顯示,10 -5 mol/L淫羊藿苷可明顯促進hDPSCs中各礦化相關基因的錶達和礦化結節的形成. 結論: 一定濃度範圍的淫羊藿苷可促進hDPSCs的增殖和骨嚮分化.
목적:탐토음양곽감대인아수간세포( hDPSCs)증식급골향분화적영향. 방법:MTT검측10 -2 ~10 -10 mol/L음양곽감대hDPSCs증식능력적영향,병사선출최가농도(10 -5 mol/L);연후이10 -5 mol/L적음양곽감작용우hDPSCs,병대기진행광화유도. 유도배양21 d후,용Real-time PCR검측hDPSCs중ALP、DMP-1、DSPP각광화상관기인적표체수평;천소홍염색관찰hDPSCs광화결절적능력. 결과:여대조조상비, 10 -2 ~10 -3 mol/L 적음양곽감대hDPSCs증식유일정적억제작용(P<0. 05),이10 -5 mol/L화10 -6 mol/L적음양곽감균능명현촉진hDPSCs적증식(P<0. 05),기중이10 -5 mol/L최호;Real-time PCR검측급천소홍염색관찰결과현시,10 -5 mol/L음양곽감가명현촉진hDPSCs중각광화상관기인적표체화광화결절적형성. 결론: 일정농도범위적음양곽감가촉진hDPSCs적증식화골향분화.
AIM:To explore the potential role of icariin in the regulation of proliferation and osteogenic dif-ferentiation of human dental pulp stem cells ( hDPSCs ) . METHODS: hDPSCs were treated by icariin at 10 -2 mol/L~10 -10 mol/L respectively. MTT assay was employed to evaluate the proliferative effect of icariin on hDP-SCs. Real time-PCR and Alizarin Red S were performed to examine the impact of icariin on osteogenic differentiation of hDPSCs. RESULTS:10 -2 mol/L~10 -3 mol/L icariin inhibited the proliferation of hDPSCs. 10 -5 mol/L icariin promoted the proliferation of hDPSCs. In addition, 10 -5 mol/L icariin could up-regulate osteogenic differentiation re-lated genes and could promote mineralized nodule formation. CONCLUSION: Icariin can enhance proliferation and osteogenic differentiation of hDPSCs at a certain concentration range.
