CAJ | 학술논문

目的:探讨Jun家族蛋白JunB、c-Jun 和JunD在小鼠釉质发育中的作用. 方法: 应用免疫组化染色检测JunB、c-Jun 和JunD在不同发育阶段小鼠牙胚成釉细胞中的表达;分别以0. 5 μg和2. 0 μg的JunB、c-Jun 和JunD转染小鼠成釉细胞后,运用RT-PCR观察其对釉原蛋白amelogenin mRNA表达的影响.结果:免疫组化染色结果显示:釉质分泌期时,JunB在成釉细胞核中无显著表达,JunD呈弱表达,而c-Jun表达明显;釉质转型期时,JunB和JunD 表达增强且JunD 早于JunB, c-Jun持续表达明显; 在釉质成熟期时, JunB、c-Jun和JunD均显著表达. RT-PCR检测显示:高剂量JunB可明显抑制amelogenin的表达(P<0. 05);低剂量c-Jun可明显促进amelogenin的表达(P<0. 05);JunD对amelogenin的表达无显著影响. 结论:在釉质发育早期,c-Jun可通过上调釉原蛋白的表达参与釉质发育. 在釉质发育后期,JunB可通过抑制釉原蛋白的分泌促进釉质的成熟. JunD对amelogenin表达无显著影响.
목적:탐토Jun가족단백JunB、c-Jun 화JunD재소서유질발육중적작용. 방법: 응용면역조화염색검측JunB、c-Jun 화JunD재불동발육계단소서아배성유세포중적표체;분별이0. 5 μg화2. 0 μg적JunB、c-Jun 화JunD전염소서성유세포후,운용RT-PCR관찰기대유원단백amelogenin mRNA표체적영향.결과:면역조화염색결과현시:유질분비기시,JunB재성유세포핵중무현저표체,JunD정약표체,이c-Jun표체명현;유질전형기시,JunB화JunD 표체증강차JunD 조우JunB, c-Jun지속표체명현; 재유질성숙기시, JunB、c-Jun화JunD균현저표체. RT-PCR검측현시:고제량JunB가명현억제amelogenin적표체(P<0. 05);저제량c-Jun가명현촉진amelogenin적표체(P<0. 05);JunD대amelogenin적표체무현저영향. 결론:재유질발육조기,c-Jun가통과상조유원단백적표체삼여유질발육. 재유질발육후기,JunB가통과억제유원단백적분비촉진유질적성숙. JunD대amelogenin표체무현저영향.
AIM:To investigate the role of Jun family proteins in enamel development of mouse. METH-ODS:Immunohistochemical staining was applied to detect the expression of JunB, c-Jun and JunD in mouse tooth germ ameloblasts. RT-PCR was employed to evaluate the effect on the mRNA expression of amelogenin after transfec-tion of the ameloblasts by JunB, C-Jun and JunD at 0. 5 μg and 2. 0 μg respectively. RESULTS: At the secretion stage, JunB was not expressed and JunD was weakly expressed in ameloblasts nuclei, but c-Jun was obviously ex-pressed. At the transition stage, JunB and JunD expression were increased and JunD was earlier than JunB. c-Jun continuously exhibited strong expression. At the maturation stage, all of the proteins were strongly expressed. Transfec-tion results showed that high dose of JunB inhibited the expression of amelogenin. Low dose of c-Jun evidently en-hanced the expression of amelogenin JunD had no significant effect on amelogenin expression. CONCLUTION:c-Jun may up-regulate the expression of amelogenin at the early stage of enamel development. JunB may suppress the secre-tion of amelogenin and promote the maturation of enamel at the late stage of enamel development. JunD has no significant effect on amelogenin.