CAJ | 학술논문

目的：本研究通过检测比较 microRNA-130b-5p (miR-130b-5p)在肾癌及癌旁正常组织之间以及肾癌细胞系和正常肾细胞系之间的表达水平,并分析其表达水平与临床病理资料之间的关系,为进一步研究 miR-130b-5p在肾癌中深入的功能和机制奠定基础.方法采用 qPCR方法检测 miR-130b-5p在42对肾癌组织和癌旁正常组织中表达量的差异,并运用秩和检验方法分析肾癌组织中 miR-130b-5p表达量与患者临床病理特征之间的相关性.培养肾癌细胞系(786-O 和ACHN)和正常肾上皮细胞系(293T)并采用 qPCR 方法检测细胞中 miR-130b-5p 的表达量.结果 miR-130b-5p在肾癌组织中的表达量明显高于癌旁正常组织(P＜0．01),且肾癌组织中miR-130b-5p表达量与患者 AJCC 临床分期呈正相关(P＝0．041),与其他临床病理资料如年龄、性别、T分期、组织类型和 Fuhrman分级未发现明显相关关系(P＞0．05).同时,miR-130b-5p 表达量在肾癌细胞系中也明显高于正常肾上皮细胞系(P＜0．01).结论 miR-130b-5p在肾癌组织和细胞系中均高表达,且其在肾癌组织中的表达水平与患者 AJCC 临床分期呈正相关,提示miR-130b-5p与肾癌的发生、发展相关,有望成为新的肾癌肿瘤标志物.
목적：본연구통과검측비교 microRNA-130b-5p (miR-130b-5p)재신암급암방정상조직지간이급신암세포계화정상신세포계지간적표체수평,병분석기표체수평여림상병리자료지간적관계,위진일보연구 miR-130b-5p재신암중심입적공능화궤제전정기출.방법채용 qPCR방법검측 miR-130b-5p재42대신암조직화암방정상조직중표체량적차이,병운용질화검험방법분석신암조직중 miR-130b-5p표체량여환자림상병리특정지간적상관성.배양신암세포계(786-O 화ACHN)화정상신상피세포계(293T)병채용 qPCR 방법검측세포중 miR-130b-5p 적표체량.결과 miR-130b-5p재신암조직중적표체량명현고우암방정상조직(P＜0．01),차신암조직중miR-130b-5p표체량여환자 AJCC 림상분기정정상관(P＝0．041),여기타림상병리자료여년령、성별、T분기、조직류형화 Fuhrman분급미발현명현상관관계(P＞0．05).동시,miR-130b-5p 표체량재신암세포계중야명현고우정상신상피세포계(P＜0．01).결론 miR-130b-5p재신암조직화세포계중균고표체,차기재신암조직중적표체수평여환자 AJCC 림상분기정정상관,제시miR-130b-5p여신암적발생、발전상관,유망성위신적신암종류표지물.
Objective To explore the function and mechanism of miR-130b-5p in renal cancer,we compared the expression level of microRNA-130b-5p (miR-130b-5p)between renal cancer and adjacent normal kidney tissues (ANTs )and between cells derived from renal cancer and normal kidney and we also analyzed the relationship between the expression level of miR-130b-5p and the clinical pathology. Methods Utilizing quantitative real-time polymerase chain reaction (qPCR)we determined the expression of miR-130b-5p in 42 paired renal cancer tissues and ANTs and we analysed the relationship between miR-130b-5p expression and clinical pathology by rank sum test.Cultivated the renal cancer cell lines (786-O and ACHN)and human normal kidney cell line HK-2 and determined the miR-130b-5p expression level of cells by qPCR. Results MiR-130b-5p was up-regulated in RCC tissues and cell lines compared with con-trol normal tissues and cell line.And the miR-130b-5p expression in RCC tissues is correlated with AJCC stage (P=0.041),but there is no correlation was found between miR-130b-5p expression and other clinical pathological variables such as age,sex,T stage,histological type and Furman grade (P>0.05). Conclusions MiR-130b-5p was up-regulated in both renal cancer tissues and cell lines and miR-130b-5p expression level was correlated to AJCC stage of renal cancer.It is suggested that miR-130b-5p plays a potential role in the initiation and development and miR-130b-5p might be a no-vel biomarker in renal cancer.