CAJ | 학술논문

目的：研究 Nrf2信号通路抑制剂 DRB 对体外培养的细粒棘球蚴原头节活力的影响。方法从自然感染细粒棘球蚴病的羊肝中获取新鲜细粒棘球蚴原头节，并于 RPMI 1640培养基体外培养。实验分为 DMSO 空白对照组，25 g/ L、50 g/ L、75 g/ L DRB 处理组。通过伊红染色，在倒置显微镜下观察原头节活力和形态变化，并绘制生长活力曲线。在扫描电镜下观察原头节超微结构变化。结果对照组和25 g/ L DRB 处理组体外作用细粒棘球蚴原头节后，不同时间原头节活力比较，差异无统计学意义（ F空白对照组＝0.542，P ﹥0.05；F25 g/ L DRB处理组＝0.658，P ﹥0.05）。50 g/ L DRB 处理组和75 g/ L DRB 处理组体外作用细粒棘球蚴原头节后，不同时间原头节活力比较，差异均有统计学意义（F 50 g/ L DRB处理组＝6.686，P ﹤0.01；F75 g/ L DRB处理组＝10.756，P ﹤0.01）。随着用药时间的延长和药物浓度的增加，DRB 对原头节活力的抑制效果增强，且原头节超微结构发生改变。结论高浓度的 Nrf2抑制剂对体外培养的细粒棘球蚴原头节有抑制作用，并且有一定的浓度和时间依赖性。Nrf2信号通路是抗氧化应激反应的关键因子，对细粒棘球蚴 Nrf2信号通路进行研究，可能为治疗细粒棘球蚴病提供一个新思路。
목적：연구 Nrf2신호통로억제제 DRB 대체외배양적세립극구유원두절활력적영향。방법종자연감염세립극구유병적양간중획취신선세립극구유원두절，병우 RPMI 1640배양기체외배양。실험분위 DMSO 공백대조조，25 g/ L、50 g/ L、75 g/ L DRB 처리조。통과이홍염색，재도치현미경하관찰원두절활력화형태변화，병회제생장활력곡선。재소묘전경하관찰원두절초미결구변화。결과대조조화25 g/ L DRB 처리조체외작용세립극구유원두절후，불동시간원두절활력비교，차이무통계학의의（ F공백대조조＝0.542，P ﹥0.05；F25 g/ L DRB처리조＝0.658，P ﹥0.05）。50 g/ L DRB 처리조화75 g/ L DRB 처리조체외작용세립극구유원두절후，불동시간원두절활력비교，차이균유통계학의의（F 50 g/ L DRB처리조＝6.686，P ﹤0.01；F75 g/ L DRB처리조＝10.756，P ﹤0.01）。수착용약시간적연장화약물농도적증가，DRB 대원두절활력적억제효과증강，차원두절초미결구발생개변。결론고농도적 Nrf2억제제대체외배양적세립극구유원두절유억제작용，병차유일정적농도화시간의뢰성。Nrf2신호통로시항양화응격반응적관건인자，대세립극구유 Nrf2신호통로진행연구，가능위치료세립극구유병제공일개신사로。
Objective To investigate the in vitro effect of Nrf2 signaling pathway inhibitor DRB on the activity of echinococcus granulosus protoscoleces. Methods We obtained fresh echinococcus granulosus protoscoleces from lamb liver naturally infected with echinococcosis granulose and conducted in vitro culture in RPMI 1640 medium. The samples were divided into four groups:DMSO control group,25 g/ L DRB group,50 g/ L DRB group and 75 g/ L DRB group. By eosin staining,the activity and morphological changes of echinococcus granulosus protoscoleces were observed under inverted microscope,and activity curves were made. The ultrastructure of protoscoleces was observed under scanning electron microscope. Results There were not significantly different in vitro effect of control group and 25 g/ L DRB group on the activity of echinococcus granulosus protoscoleces in different time(Fcontrol = 0. 542,P ﹥ 0. 05;F25 g/ L DRB = 0. 658,P ﹥ 0. 05). There were significantly different in vitro effect of 50 g/ L DRB group and 75 g/ L DRB group on the activity of echinococcus granulosus protoscoleces in different time (F 50 g/ L DRB = 6. 686,P ﹤ 0. 01;F75 g/ L DRB = 10. 756,P ﹤ 0. 01). With the lengthening of medication time and the increase of drug concentration,the inhibiting effect of DRB on protoscoleces vitality was strengthened,and the ultrastructure was changed. Conclusion High concentrations of DRB inhibitors can all inhibit in vitro cultured echinococcus granulosus protoscoleces,the dependence on concentration and time length exists. Nrf2 signaling pathway is a key factor for antioxidant stress. The study on the Nrf2 signaling pathway of echinococcus granulosus may provide a new thought for the treatment of echinococcus granulosus.