现代泌尿生殖肿瘤杂志
現代泌尿生殖腫瘤雜誌
현대비뇨생식종류잡지
Journal of Contemporary Urologic and Reproductive Oncology
2015年
4期
236-239
,共4页
胡祎民%金露%李一帆%陈独群%苏郑明%刘家驹%杨尚琪%桂耀庭%毛向明%来永庆
鬍祎民%金露%李一帆%陳獨群%囌鄭明%劉傢駒%楊尚琪%桂耀庭%毛嚮明%來永慶
호의민%금로%리일범%진독군%소정명%류가구%양상기%계요정%모향명%래영경
肾肿瘤%miR-10b%肿瘤分期
腎腫瘤%miR-10b%腫瘤分期
신종류%miR-10b%종류분기
Kidney neoplasms%miR-10b%Neoplasm staging
目的：检测35例肾癌标本及配对正常组织标本中 miR-10b 的表达,分析 miR-10b 表达水平与患者临床特征之间的关系,为 miR-10b后续功能研究奠定基础.方法提取35例手术切除的肾癌及癌旁正常组织标本中的总 RNA,反转录获得 cDNA,实时荧光定量 PCR(qRT-PCR)检测 miR-10b的表达水平,统计分析其表达水平与患者临床病理特征之间的关系.结果35例组织标本中26例(74．29％)肾癌组织 miR-10b表达水平下降(P＜0．05),且下降和患者年龄、性别、肿瘤分期分级等无明显相关(P＞0．05).结论 miR-10b 在肾癌组织中表达明显下调,提示其与肾癌发生有一定关系.
目的：檢測35例腎癌標本及配對正常組織標本中 miR-10b 的錶達,分析 miR-10b 錶達水平與患者臨床特徵之間的關繫,為 miR-10b後續功能研究奠定基礎.方法提取35例手術切除的腎癌及癌徬正常組織標本中的總 RNA,反轉錄穫得 cDNA,實時熒光定量 PCR(qRT-PCR)檢測 miR-10b的錶達水平,統計分析其錶達水平與患者臨床病理特徵之間的關繫.結果35例組織標本中26例(74．29％)腎癌組織 miR-10b錶達水平下降(P＜0．05),且下降和患者年齡、性彆、腫瘤分期分級等無明顯相關(P＞0．05).結論 miR-10b 在腎癌組織中錶達明顯下調,提示其與腎癌髮生有一定關繫.
목적：검측35례신암표본급배대정상조직표본중 miR-10b 적표체,분석 miR-10b 표체수평여환자림상특정지간적관계,위 miR-10b후속공능연구전정기출.방법제취35례수술절제적신암급암방정상조직표본중적총 RNA,반전록획득 cDNA,실시형광정량 PCR(qRT-PCR)검측 miR-10b적표체수평,통계분석기표체수평여환자림상병리특정지간적관계.결과35례조직표본중26례(74．29％)신암조직 miR-10b표체수평하강(P＜0．05),차하강화환자년령、성별、종류분기분급등무명현상관(P＞0．05).결론 miR-10b 재신암조직중표체명현하조,제시기여신암발생유일정관계.
Objective To detect the expression levels of miR-10b in 35 RCC tissues and paired normal tissues and explore the relationship between the miR-10b expression and clinical characteris-tics of patients for further study of the function of miR-10b in RCC. Methods Total RNA isolated from 35 paired tissues obtained from nephrectomy were used to get cDNA by performing reverse transcription.The expression level of miR-10b was detected by quantitative real-time PCR (qRT-PCR)and the relationships with clinical characteristic were explored by statistical analysis. Results 26 cases (74.29%)showed down-regulation of miR-10b (P<0.05).No relationships were found between the expression of miR-10b and patient′s age,clinical stage or grade. Conclusions miR-10b is down-regulated in RCC,indicating it may be involved in the tumorigenesis of RCC.
