CAJ | 학술논문

以甲醛为唯一碳源和能源，从皮革废水处理厂的活性污泥中，分离得到一株甲醛高效降解菌JQ-6，结合形态学特征、生理生化特性研究和16S rDNA序列鉴定显示，JQ-6属于恶臭假单胞菌（Pseudomonas putida）.通过单因素试验和正交试验，检测不同培养条件对菌株降解甲醛的影响，得出菌株JQ-6降解甲醛的最佳条件为：甲醛1000 mg/L，30℃、pH6.0、摇床转速150 r/min、100 mL培养基JQ-6菌悬液的接种量为3 mL，在此条件下培养24 h后，菌株JQ-6对甲醛的降解率可以达到70%以上.
이갑철위유일탄원화능원，종피혁폐수처리엄적활성오니중，분리득도일주갑철고효강해균JQ-6，결합형태학특정、생리생화특성연구화16S rDNA서렬감정현시，JQ-6속우악취가단포균（Pseudomonas putida）.통과단인소시험화정교시험，검측불동배양조건대균주강해갑철적영향，득출균주JQ-6강해갑철적최가조건위：갑철1000 mg/L，30℃、pH6.0、요상전속150 r/min、100 mL배양기JQ-6균현액적접충량위3 mL，재차조건하배양24 h후，균주JQ-6대갑철적강해솔가이체도70%이상.
A bacterial strain(JQ-6)capable to degrade and metabolize formaldehyde as a sole carbon and energy sources was isolated from the collected activated sludge of leather wastewater treatment plants. The results of morpho-logical features,physiological and biochemical characters as well as 16S rDNA sequence analysis of the bacterium showed that,the JQ-6 strain was Pseudomonas putida. Single-factor and orthogonal experiments were employed to study the capability of degradation characteristics of strain JQ-6. The optimum conditions of formaldehyde degradation were as follow:formaldehyde concentration of 1000 mg/L,incubation temperature of 30℃,pH 6.0,rotating rate of 150 r/min,100 mL fresh medium seeded with 3 mL JQ-6 bacterial suspension. The formaldehyde degradation rate reached 70%within 24 h by this strain.