中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
Chinese Journal of Hepatobiliary Surgery
2015年
9期
629-632
,共4页
池其煜%黄鹤光%王丛菲%谢捷明%胡伟泽%付明娟%陈明晃
池其煜%黃鶴光%王叢菲%謝捷明%鬍偉澤%付明娟%陳明晃
지기욱%황학광%왕총비%사첩명%호위택%부명연%진명황
南瓜蛋白%胰腺癌%化疗药物%细胞增殖%协同作用
南瓜蛋白%胰腺癌%化療藥物%細胞增殖%協同作用
남과단백%이선암%화료약물%세포증식%협동작용
Cucurmosin%Pancreatic cancer%Chemotherapeutic drugs%Cell Proliferation%Synergistic effect
目的 研究南瓜蛋白(CUS)联合胰腺癌常用化疗药物吉西他滨(GEM)、氟尿嘧啶(5-FU)、紫杉醇(PTX)、顺铂(DDP)对人胰腺癌细胞株BxPC-3的增殖抑制作用.方法 采用磺酰罗丹明B(SRB)比色法测定CUS分别与GEM、5-FU、PTX、DDP联用对体外培养的BxPC-3细胞的增殖抑制作用;应用集落形成实验测定CUS分别与GEM、5-FU、PTX、DDP联用对体外培养的BxPC-3细胞集落形成的影响;用金氏公式判断药物联用的协同效果.结果 CUS联用GEM、5-FU、PTX、DDP对BxPC-3细胞的增殖抑制率均大于各药单用的抑制率,q＞0.85;CUS与胰腺癌常用化疗药物均在低浓度范围内联用的相加效果明显.CUS联用GEM、5-FU 、PTX、DDP对BxPC-3细胞的集落形成抑制率均大于各药单用的抑制率,q＞ 1.15.结论 CUS联合GEM、5-FU、PTX、DDP常用化疗药物对体外培养的BxPC-3细胞的生长抑制具有较好的协同作用.
目的 研究南瓜蛋白(CUS)聯閤胰腺癌常用化療藥物吉西他濱(GEM)、氟尿嘧啶(5-FU)、紫杉醇(PTX)、順鉑(DDP)對人胰腺癌細胞株BxPC-3的增殖抑製作用.方法 採用磺酰囉丹明B(SRB)比色法測定CUS分彆與GEM、5-FU、PTX、DDP聯用對體外培養的BxPC-3細胞的增殖抑製作用;應用集落形成實驗測定CUS分彆與GEM、5-FU、PTX、DDP聯用對體外培養的BxPC-3細胞集落形成的影響;用金氏公式判斷藥物聯用的協同效果.結果 CUS聯用GEM、5-FU、PTX、DDP對BxPC-3細胞的增殖抑製率均大于各藥單用的抑製率,q＞0.85;CUS與胰腺癌常用化療藥物均在低濃度範圍內聯用的相加效果明顯.CUS聯用GEM、5-FU 、PTX、DDP對BxPC-3細胞的集落形成抑製率均大于各藥單用的抑製率,q＞ 1.15.結論 CUS聯閤GEM、5-FU、PTX、DDP常用化療藥物對體外培養的BxPC-3細胞的生長抑製具有較好的協同作用.
목적 연구남과단백(CUS)연합이선암상용화료약물길서타빈(GEM)、불뇨밀정(5-FU)、자삼순(PTX)、순박(DDP)대인이선암세포주BxPC-3적증식억제작용.방법 채용광선라단명B(SRB)비색법측정CUS분별여GEM、5-FU、PTX、DDP련용대체외배양적BxPC-3세포적증식억제작용;응용집락형성실험측정CUS분별여GEM、5-FU、PTX、DDP련용대체외배양적BxPC-3세포집락형성적영향;용금씨공식판단약물련용적협동효과.결과 CUS련용GEM、5-FU、PTX、DDP대BxPC-3세포적증식억제솔균대우각약단용적억제솔,q＞0.85;CUS여이선암상용화료약물균재저농도범위내련용적상가효과명현.CUS련용GEM、5-FU 、PTX、DDP대BxPC-3세포적집락형성억제솔균대우각약단용적억제솔,q＞ 1.15.결론 CUS연합GEM、5-FU、PTX、DDP상용화료약물대체외배양적BxPC-3세포적생장억제구유교호적협동작용.
Objective To investigate the inhibitory effect of cucurmosin (CUS) combined with the commonly used chemotherapeutic drugs for pancreatic cancer in clinical practice including Gemcitabine (GEM),Fluorouracil (5-FU),Paclitaxel (PTX) and Cisplatin (DDP) on cell proliferation of human pancreatic cancer cell line BxPC-3.Mehtods Sulforhodamine B (SRB) assay was used to detect the inhibition on the cell proliferation of BxPC-3 cells in vitro after the treatment of CUS combined with GEM,5-FU,PTX and DDP,respectively.Colony formation assay was also conducted and Jin' s formula was used to assess the synergistic effect of these combinations.Results The inhibition rate of CUS combined with GEM,5-FU,PTX and DDP were all higher than those of each drug alone (q ＞ 0.85),which became obvious in low concentrations.The colony formation inhibition rate of CUS combined with GEM,5-FU,PTX or DDP were all higher than each single drug treatment (q ＞ 1.15).Conclusion CUS could enhance the cell growth inhibition of GEM,5-FU,PTX and DDP in BxPC-3 cells in vitro with a good synergistic effect.
