中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
Chinese Journal of Hepatobiliary Surgery
2015年
9期
625-628
,共4页
李弦%王伟%胡晓燕%王彦峰%熊艳%叶啟发
李絃%王偉%鬍曉燕%王彥峰%熊豔%葉啟髮
리현%왕위%호효연%왕언봉%웅염%협계발
低温机械灌注%肝糖原%阻力指数%丙二醛%供肝%大鼠
低溫機械灌註%肝糖原%阻力指數%丙二醛%供肝%大鼠
저온궤계관주%간당원%조력지수%병이철%공간%대서
Hypothermic machine perfusion%Liver glycogen content%Resistance index%Malondialdehyde%Donor liver%Rats
目的 探索缺血30 min后肝细胞代谢变化及低温机械灌注对肝细胞代谢的影响.方法 将40只SD雄性大鼠随机均分为A、B、C、D四组.A组供肝热缺血0 min+冷保存5h;B组热缺血0 min+冷保存4h+机械灌注1h;C组供肝热缺血30 min+冷保存5h;D组供肝热缺血30 min+冷保存4h+机械灌注1h.在机械灌注过程中每隔10 min记录阻力指数值.完成灌注后切取0.2g肝组织检测肝糖原含量及丙二醛(MDA)值.结果 经机械灌注处理后肝糖原含量较冷保存有所降低,但差异无统计学意义(P>0.05);A、B两组MDA含量无差异(P>0.05);D组MDA含量明显高于C组(P<0.05);经机械灌注处理后,肝脏阻力指数值均有明显降低.结论 肝脏遭受30 min热缺血损伤后,再行机械灌注,可降低其阻力指数,增加肝细胞内细胞代谢,使得脂质过氧化物生成增多.
目的 探索缺血30 min後肝細胞代謝變化及低溫機械灌註對肝細胞代謝的影響.方法 將40隻SD雄性大鼠隨機均分為A、B、C、D四組.A組供肝熱缺血0 min+冷保存5h;B組熱缺血0 min+冷保存4h+機械灌註1h;C組供肝熱缺血30 min+冷保存5h;D組供肝熱缺血30 min+冷保存4h+機械灌註1h.在機械灌註過程中每隔10 min記錄阻力指數值.完成灌註後切取0.2g肝組織檢測肝糖原含量及丙二醛(MDA)值.結果 經機械灌註處理後肝糖原含量較冷保存有所降低,但差異無統計學意義(P>0.05);A、B兩組MDA含量無差異(P>0.05);D組MDA含量明顯高于C組(P<0.05);經機械灌註處理後,肝髒阻力指數值均有明顯降低.結論 肝髒遭受30 min熱缺血損傷後,再行機械灌註,可降低其阻力指數,增加肝細胞內細胞代謝,使得脂質過氧化物生成增多.
목적 탐색결혈30 min후간세포대사변화급저온궤계관주대간세포대사적영향.방법 장40지SD웅성대서수궤균분위A、B、C、D사조.A조공간열결혈0 min+랭보존5h;B조열결혈0 min+랭보존4h+궤계관주1h;C조공간열결혈30 min+랭보존5h;D조공간열결혈30 min+랭보존4h+궤계관주1h.재궤계관주과정중매격10 min기록조력지수치.완성관주후절취0.2g간조직검측간당원함량급병이철(MDA)치.결과 경궤계관주처리후간당원함량교랭보존유소강저,단차이무통계학의의(P>0.05);A、B량조MDA함량무차이(P>0.05);D조MDA함량명현고우C조(P<0.05);경궤계관주처리후,간장조력지수치균유명현강저.결론 간장조수30 min열결혈손상후,재행궤계관주,가강저기조력지수,증가간세포내세포대사,사득지질과양화물생성증다.
Objective To examine the metabolic variations in liver after 30 min warm ischemic injury,and the effect of hypothermic machine perfusion on the metabolism in rats.Methods 40 SD male rats were randomly divided into 4 groups:Group A,the liver underwent warm ischemia for 0 min and cold storage (CS) for5 h; Group B,the liver was treated by warm ischemia for0 min,CS for4 h and then machine perfusion (MP) for 1 h; Group C,the liver suffered from warm ischemia for 30 min and CS for 5 h; and Group D,the liver was treated by warm ischemia for 30 min plus CS for 4 h plus MP for 1 h.During the MP process,the perfusion resistance index was recorded every 10 min,and the liver glycogen content and malondialdehyde (MDA) value were also detected.Results The hepatic glycogen content decreased after MP treatment,but there was no statistical significance (P > 0.05).No differences on MDA contents was found between Group A and B (P > 0.05),while MDA in Group D was significantly higher than that in Group C (P < 0.05).After hypothermic MP treatment,the liver resistance index value was significantly reduced.Conclusion MP could reduce the resistance index but increase metabolic rate in liver undergoing warm ischemic injury,thus producing more lipid peroxides.