CAJ | 학술논문

目的研究SLE患者全基因组羟基化水平,寻找SLE患者与健康对照5-羟甲基胞嘧啶(5-hmC)水平差异显著的目的基因,作为SLE特异性生物标志物.方法应用hMeDIP-chip技术,分别检测SLE患者15例和健康对照组15名外周血的DNA 5-hmC状态.通过GO功能分析、pathway分析等分析方法对比分析,筛选出羟甲基化水平差异显著的目的基因,并用实时定量PCR技术验证hMeDIP-chip结果可靠性.结果 NanoDrop ND-1000和琼脂糖凝胶电泳证明所提的样本总DNA质量良好,可用于后续实验.与健康对照组相比,SLE患者在启动子区有1 701个5-hmC差异基因,其中884个上调,817个下调;在CPG岛有3 826个5-hmC差异基因,其中2 034个上调,1 792个下调.挑选3个差异最大的基因:TREX1、CDKN1A、CDKN1B进行讨论,并进行实时定量PCR验证实验,其结果与hMeDIP-chip结果相符,证明了hMeDIP-chip结果的可靠性.结论研究结果表明SLE患者的5-hmC状态有很大的变化,这些变化或许可以进一步揭示SLE的发病机制.这些新发现也表明了5-hmC可以作为SLE的潜在生物标志物和治疗靶点.
목적 연구SLE환자전기인조간기화수평,심조SLE환자여건강대조5-간갑기포밀정(5-hmC)수평차이현저적목적기인,작위SLE특이성생물표지물.방법 응용hMeDIP-chip기술,분별검측SLE환자15례화건강대조조15명외주혈적DNA 5-hmC상태.통과GO공능분석、pathway분석등분석방법대비분석,사선출간갑기화수평차이현저적목적기인,병용실시정량PCR기술험증hMeDIP-chip결과가고성.결과 NanoDrop ND-1000화경지당응효전영증명소제적양본총DNA질량량호,가용우후속실험.여건강대조조상비,SLE환자재계동자구유1 701개5-hmC차이기인,기중884개상조,817개하조;재CPG도유3 826개5-hmC차이기인,기중2 034개상조,1 792개하조.도선3개차이최대적기인:TREX1、CDKN1A、CDKN1B진행토론,병진행실시정량PCR험증실험,기결과여hMeDIP-chip결과상부,증명료hMeDIP-chip결과적가고성.결론 연구결과표명SLE환자적5-hmC상태유흔대적변화,저사변화혹허가이진일보게시SLE적발병궤제.저사신발현야표명료5-hmC가이작위SLE적잠재생물표지물화치료파점.
Objective To investigate the role of the 5-hydroxymethylcytosine (5-hmC) DNA modification in the onset of systemic lupus erythemosus (SLE),we compared tihe levels 5-hmC between SLE patients and normal controls.Methods With informed consent,whole blood was obtained from patients,and genomic DNA was extracted.Using hMeDIP-seq analysis and validation by quantitative real-time quantitative polymerase chain reaction (RT-PCR),we identified the differentially hydroxymethylated regions that were associated with SLE.Results There were 1 701 genes with significantly different 5-hmC levels at the promoter region in the SLE patients compared with the normal controls.The CpG islands of 3 826 genes showed significant difference at 5-hmC levels in SLE patients compared with the normal controls.Out of the differentially hydroxymethylated genes,three were selected for validation,including TREX1,CDKN1A,and CDKN1B.The hydroxymethylation levels of these three genes were confirmed by quantitative RT-PCR.Conclusion Our studies indicate that there are significant alterations of 5-hmC in SLE patients;these differentially hydroxymethylated genes may contribute to the pathogenesis of SLE.Such novel findings show the significance of 5-hmC as a potential biomarker or promising target for epigenetic-based SLE therapies.