中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
9期
646-649
,共4页
刘丹%蔺新英%于福贵%张曼
劉丹%藺新英%于福貴%張曼
류단%린신영%우복귀%장만
碘%大鼠%甲状腺功能亢进症%心肌%Na+-K+-ATP酶%Mg2+-ATP酶%Ca2+-ATP酶
碘%大鼠%甲狀腺功能亢進癥%心肌%Na+-K+-ATP酶%Mg2+-ATP酶%Ca2+-ATP酶
전%대서%갑상선공능항진증%심기%Na+-K+-ATP매%Mg2+-ATP매%Ca2+-ATP매
Iodine%Rats%Hyperthyroidism%Myocardium%Na+-K+-ATPase%Mg2+-ATPase%Ca2+-ATPase
目的 观察海带有机碘(DIT)与无机碘(KI)对甲状腺功能亢进(简称甲亢)形成中的Wistar大鼠心肌ATP酶活性的影响.方法 雄性Wistar大鼠72只,按体质量采用随机数字表法分成8组(每组9只),分别为对照组,甲亢模型组,DIT和KI低、中、高剂量组(DIT和KI均为25.0、166.7、500.1μg/kg).对照组大鼠灌胃生理盐水;甲亢模型组灌胃甲状腺片悬浊液(200.0 mg/kg);DIT和KI低、中、高剂量组在给予相应剂量的碘同时灌胃甲状腺片悬浊液.每天灌胃1次,连续1个月,断头处死各组大鼠取心脏组织,比色法检测各组大鼠心肌钠-钾(Na+-K+)-ATP酶、镁离子(Mg2+)-ATP酶、钙离子(Ca2+)-ATP酶的活性.结果 各组间大鼠心肌Na+-K+-ATP酶、Mg2+-ATP酶、Ca2+-ATP酶活性比较差异均有统计学意义(F=2.99、3.03、6.18,P均＜0.01).其中与对照组[(4.01±0.22)、(4.28±0.28)、(4.46±0.35) μmol/mg·h]比较,甲亢模型组3种心肌ATP酶活性[(3.60±0.25)、(3.42±0.31)、(3.85±0.17) μmol/mg·h]显著降低(P均＜0.01);DIT中剂量组Mg2+-ATP酶[(3.89±0.35) μmol/mg·h]和中、高剂量组Ca2+-ATP酶[(4.12±0.20)、(4.09±0.21) μmol/mg·h]活性降低(P均＜0.05);KI低、中、高剂量组Na+-K+-ATP酶[(3.64±0.32)、(3.60±0.32)、(3.53±0.33) μmol/mg·h]、Ca2+-ATP酶[(3.93±0.22)、(3.90±0.23)、(3.85±0.26) μmol/mg·h],高剂量组Mg2+-ATP酶活性[(3.65±0.49)μmol/mg·h]显著降低(P＜ 0.05或＜0.01).与甲亢模型组比较,DIT低、中剂量组Mg2+-ATP酶[(4.06±0.51)、(3.89±0.35) μmol/mg·h]活性,低、中、高剂量组Ca2+-ATP酶[(4.15±0.26)、(4.12±0.20)、(4.09±0.21)μmol/mg·h]活性升高(P均＜0.05).结论 大鼠在甲亢形成过程中补充相同剂量的碘剂时,DIT对大鼠心肌损害要小于同剂量的KI.
目的 觀察海帶有機碘(DIT)與無機碘(KI)對甲狀腺功能亢進(簡稱甲亢)形成中的Wistar大鼠心肌ATP酶活性的影響.方法 雄性Wistar大鼠72隻,按體質量採用隨機數字錶法分成8組(每組9隻),分彆為對照組,甲亢模型組,DIT和KI低、中、高劑量組(DIT和KI均為25.0、166.7、500.1μg/kg).對照組大鼠灌胃生理鹽水;甲亢模型組灌胃甲狀腺片懸濁液(200.0 mg/kg);DIT和KI低、中、高劑量組在給予相應劑量的碘同時灌胃甲狀腺片懸濁液.每天灌胃1次,連續1箇月,斷頭處死各組大鼠取心髒組織,比色法檢測各組大鼠心肌鈉-鉀(Na+-K+)-ATP酶、鎂離子(Mg2+)-ATP酶、鈣離子(Ca2+)-ATP酶的活性.結果 各組間大鼠心肌Na+-K+-ATP酶、Mg2+-ATP酶、Ca2+-ATP酶活性比較差異均有統計學意義(F=2.99、3.03、6.18,P均＜0.01).其中與對照組[(4.01±0.22)、(4.28±0.28)、(4.46±0.35) μmol/mg·h]比較,甲亢模型組3種心肌ATP酶活性[(3.60±0.25)、(3.42±0.31)、(3.85±0.17) μmol/mg·h]顯著降低(P均＜0.01);DIT中劑量組Mg2+-ATP酶[(3.89±0.35) μmol/mg·h]和中、高劑量組Ca2+-ATP酶[(4.12±0.20)、(4.09±0.21) μmol/mg·h]活性降低(P均＜0.05);KI低、中、高劑量組Na+-K+-ATP酶[(3.64±0.32)、(3.60±0.32)、(3.53±0.33) μmol/mg·h]、Ca2+-ATP酶[(3.93±0.22)、(3.90±0.23)、(3.85±0.26) μmol/mg·h],高劑量組Mg2+-ATP酶活性[(3.65±0.49)μmol/mg·h]顯著降低(P＜ 0.05或＜0.01).與甲亢模型組比較,DIT低、中劑量組Mg2+-ATP酶[(4.06±0.51)、(3.89±0.35) μmol/mg·h]活性,低、中、高劑量組Ca2+-ATP酶[(4.15±0.26)、(4.12±0.20)、(4.09±0.21)μmol/mg·h]活性升高(P均＜0.05).結論 大鼠在甲亢形成過程中補充相同劑量的碘劑時,DIT對大鼠心肌損害要小于同劑量的KI.
목적 관찰해대유궤전(DIT)여무궤전(KI)대갑상선공능항진(간칭갑항)형성중적Wistar대서심기ATP매활성적영향.방법 웅성Wistar대서72지,안체질량채용수궤수자표법분성8조(매조9지),분별위대조조,갑항모형조,DIT화KI저、중、고제량조(DIT화KI균위25.0、166.7、500.1μg/kg).대조조대서관위생리염수;갑항모형조관위갑상선편현탁액(200.0 mg/kg);DIT화KI저、중、고제량조재급여상응제량적전동시관위갑상선편현탁액.매천관위1차,련속1개월,단두처사각조대서취심장조직,비색법검측각조대서심기납-갑(Na+-K+)-ATP매、미리자(Mg2+)-ATP매、개리자(Ca2+)-ATP매적활성.결과 각조간대서심기Na+-K+-ATP매、Mg2+-ATP매、Ca2+-ATP매활성비교차이균유통계학의의(F=2.99、3.03、6.18,P균＜0.01).기중여대조조[(4.01±0.22)、(4.28±0.28)、(4.46±0.35) μmol/mg·h]비교,갑항모형조3충심기ATP매활성[(3.60±0.25)、(3.42±0.31)、(3.85±0.17) μmol/mg·h]현저강저(P균＜0.01);DIT중제량조Mg2+-ATP매[(3.89±0.35) μmol/mg·h]화중、고제량조Ca2+-ATP매[(4.12±0.20)、(4.09±0.21) μmol/mg·h]활성강저(P균＜0.05);KI저、중、고제량조Na+-K+-ATP매[(3.64±0.32)、(3.60±0.32)、(3.53±0.33) μmol/mg·h]、Ca2+-ATP매[(3.93±0.22)、(3.90±0.23)、(3.85±0.26) μmol/mg·h],고제량조Mg2+-ATP매활성[(3.65±0.49)μmol/mg·h]현저강저(P＜ 0.05혹＜0.01).여갑항모형조비교,DIT저、중제량조Mg2+-ATP매[(4.06±0.51)、(3.89±0.35) μmol/mg·h]활성,저、중、고제량조Ca2+-ATP매[(4.15±0.26)、(4.12±0.20)、(4.09±0.21)μmol/mg·h]활성승고(P균＜0.05).결론 대서재갑항형성과정중보충상동제량적전제시,DIT대대서심기손해요소우동제량적KI.
Objective To study the effects of 3,5-diiodotyrosine (DIT) and potassium iodide (KI) on myocardial ATPase activity in hyperthyroidism Wistar rats induced by thyroid tablets.Methods Seventy-two Wistar rats were divided into 8 groups according to body weight by the random number table method (9 rats in each group),respectively,which were control group,hyperthyroidism model group,low,medium and high doses groups (both DIT and KI contents were 25.0,166.7,500.1 μg/kg).Physiological saline was intragastrically administrated to the control group;the hyperthyroidism model group was given thyroid tablet suspension (200.0 mg/kg);DIT and KI groups were given thyroid tablet suspension with corresponding doses of iodine simultaneously.The medicine was given once a day for a mouth,all the rats were sacrificed and heart tissue was collected.The colorimetric method was used to examine the activity of ATPases (Na+-K+-ATPase,Mg2+-ATPase,Ca2+-ATPase).Results The activities of Na+-K+-ATPase,Mg2+-ATPase,Ca2+-ATPase were significantly different statistically between groups (F =2.99,3.03,6.18,all P ＜ 0.01).Compared with the control group [(4.01 ± 0.22),(4.28 ± 0.28),(4.46 ± 0.35) μmol/mg·h],the activities of ATPases (Na+-K+-ATPase,Mg2+-ATPase,Ca2+-ATPase included) were reduced significantly in hyperthyroidism model group [(3.60 ± 0.25),(3.42 ± 0.31),(3.85 ± 0.17)μ mol/mg·h,all P ＜ 0.01];the activities of Mg2+-ATPase in DIT medium dose group [(3.89 ± 0.35)μmol/mg ·h],Ca2+-ATPase in DIT medium and high doses groups [(4.12 ± 0.20),(4.09 ± 0.21)μ mol/mg·h] were reduced significantly (all P ＜ 0.05);the activities of Na+-K+-ATPases,Ca2+-ATPase were decreased significantly in three KI groups [(3.64 ± 0.32),(3.60 ± 0.32),(3.53 ± 0.33),(3.93 ± 0.22),(3.90 ± 0.23),(3.85 ± 0.26)μmol/mg·h],Mg2+-ATPase in KI high dose group [(3.65 ± 0.49)μmol/mg·h] was decreased significantly (P ＜ 0.05or ＜ 0.01).Compared with the hyperthyroidism model group,the activities of ATPase were increased in most of the DIT groups [Mg2+-ATPase in low,medium doses groups:(4.06 ± 0.51),(3.89 ± 0.35)μmol/mg·h;Ca2+-ATPase in low,medium,high doses groups (4.15 ± 0.26),(4.12 ± 0.20),(4.09 ± 0.21)μmol/mg·h,all P ＜ 0.05].Conclusion Supplementation of thyroid tablets in the process of hyperthyroidism formation in Wistar rats will reduce myocardial damage by DTT compared with the same dose of KI.
