CAJ | 학술논문

目的观察高碘对哺乳期甲状腺疾病患者外周血促甲状腺激素受体(TSHR)和蛋白激酶A(PKA)及钠碘同向转运体(NIS)mRNA表达的影响.方法在山西省适碘和高碘地区,抽取99名哺乳妇女作为观察对象,并按照是否患有甲状腺疾病分为病例组和对照组(高碘地区病例组21名、高碘地区对照组19名、适碘地区病例组30名、适碘地区对照组29名).采集所有观察对象外周血,采用实时荧光定量PCR法检测TSHR、PKA、NIS mRNA表达.结果高碘地区病例组[中位数(M):0.099、0.994]和对照组(M:0.240、0.738)分别与适碘地区病例组(M:3.087、1.127)和对照组(M:1.823、0.842)比较,TSHR mRNA表达明显降低(Z=-5.034、-4.010,P均＜ 0.01),PKA mRNA表达有降低趋势,但差异无统计学意义(Z=2.895、-0.343,P均＞0.05).高碘地区病例组NIS mRNA表达(M:0.485)明显低于适碘地区病例组(M:2.680,Z=-3.311,P＜ 0.01),而高碘地区对照组(M:0.470)与适碘地区对照组(M:0.835)比较,差异无统计学意义(Z=-1.882,P＞0.05).NIS、TSHR mRNA表达呈正相关[相关系数(r)=0.741,P＜ 0.01];NIS、PKA mRNA表达也呈正相关(r=0.293,P＜ 0.01);但TSHR、PKA mRNA表达未见统计学相关性(r=-0.081,P＞0.05).结论哺乳期妇女可能通过促甲状腺激素(TSH)-TSHR-环磷酸腺苷(cAMP)-PKA信号通路调控机体的摄碘水平来达到保护自身及子代的目的.
목적 관찰고전대포유기갑상선질병환자외주혈촉갑상선격소수체(TSHR)화단백격매A(PKA)급납전동향전운체(NIS)mRNA표체적영향.방법 재산서성괄전화고전지구,추취99명포유부녀작위관찰대상,병안조시부환유갑상선질병분위병례조화대조조(고전지구병례조21명、고전지구대조조19명、괄전지구병례조30명、괄전지구대조조29명).채집소유관찰대상외주혈,채용실시형광정량PCR법검측TSHR、PKA、NIS mRNA표체.결과 고전지구병례조[중위수(M):0.099、0.994]화대조조(M:0.240、0.738)분별여괄전지구병례조(M:3.087、1.127)화대조조(M:1.823、0.842)비교,TSHR mRNA표체명현강저(Z=-5.034、-4.010,P균＜ 0.01),PKA mRNA표체유강저추세,단차이무통계학의의(Z=2.895、-0.343,P균＞0.05).고전지구병례조NIS mRNA표체(M:0.485)명현저우괄전지구병례조(M:2.680,Z=-3.311,P＜ 0.01),이고전지구대조조(M:0.470)여괄전지구대조조(M:0.835)비교,차이무통계학의의(Z=-1.882,P＞0.05).NIS、TSHR mRNA표체정정상관[상관계수(r)=0.741,P＜ 0.01];NIS、PKA mRNA표체야정정상관(r=0.293,P＜ 0.01);단TSHR、PKA mRNA표체미견통계학상관성(r=-0.081,P＞0.05).결론 포유기부녀가능통과촉갑상선격소(TSH)-TSHR-배린산선감(cAMP)-PKA신호통로조공궤체적섭전수평래체도보호자신급자대적목적.
Objective To observe the effect of high iodine on mRNA expression of thyroid hormone receptor (TSHR),protein kinase A (PKA) and sodium iodide symporter (NIS) in peripheral blood of patients with thyroid diseases during lacatation.Methods A total of 99 breast-feeding women were selected as observation objects in Shanxi Province's sufficient iodine and high iodine areas,and they were divided into case group and control group according to whether suffer from thyroid disease.In high iodine areas,there were 21 patients and 19 healthy controls.In sufficient iodine areas,there were 30 patients and 29 healthy controls.Peripheral blood of all the observation objects was collected,and mRNA expression of TSHR,PKA and NIS was detected by real-time quantitative PCR.Results The case group [median (M):0.099,0.994] and the control group (M:0.240,0.738) in the high iodine areas were respectively compared with the case group (M:3.087,1.127) and the control group (M:1.823,0.842) in the sufficient iodine areas.The TSHR mRNA expression was significantly decreased (Z =-5.034,-4.010,all P ＜ 0.01);the PKA mRNA expression had a downward trend,and the difference was not statistically significant (Z =2.895,-0.343,all P＞ 0.05).The NIS mRNA expression of the case group in high iodine areas (M:0.485) was obviously lower than that of the the case group in sufficient iodine regions (M:2.680,Z=-3.311,P ＜ 0.01).The control group in high iodine areas (M:0.470) was compared with the control group in sufficient iodine areas (M:0.835),and the difference was not statistically significant (Z =-1.882,P ＞ 0.05).The NIS and the TSHR mRNA were positively correlated [correlation coefficient (r) =0.741,P ＜ 0.01];the NIS and the PKA mRNA was also positively correlated (r =0.293,P ＜ 0.01);but the TSHR mRNA was not significantly correlated with the PKA mRNA (r =-0.081,P ＞ 0.05).Conclusion Lactating women may have protected themselves and their babies through TSH-TSHR-cAMP-PKA signaling pathway that regulating iodine levels.