药学研究
藥學研究
약학연구
Journal of Pharmaceutical Research
2015年
10期
579-580,607
,共3页
反相高效液相色谱法%三七接骨丸%芍药苷
反相高效液相色譜法%三七接骨汍%芍藥苷
반상고효액상색보법%삼칠접골환%작약감
RP - HPLC%Sanqi Jiegu Pills%Paeoniflorin
目的：建立高效液相色谱法测定三七接骨丸中芍药苷的含量。方法采用 Kromasil 100- A C18（4.6 mm ×250 mm，5μm）色谱柱，流动相为乙腈-0.1%磷酸溶液（14：86），柱温30℃，检测波长230 nm。结果芍药苷进样量在0.1182~1.1820μg 范围内呈良好的线性关系，平均加样回收率为99.12%，RSD 为0.66%。结论该方法操作简便，重现性好，结果准确，可用于三七接骨丸中芍药苷的含量测定。
目的：建立高效液相色譜法測定三七接骨汍中芍藥苷的含量。方法採用 Kromasil 100- A C18（4.6 mm ×250 mm，5μm）色譜柱，流動相為乙腈-0.1%燐痠溶液（14：86），柱溫30℃，檢測波長230 nm。結果芍藥苷進樣量在0.1182~1.1820μg 範圍內呈良好的線性關繫，平均加樣迴收率為99.12%，RSD 為0.66%。結論該方法操作簡便，重現性好，結果準確，可用于三七接骨汍中芍藥苷的含量測定。
목적：건립고효액상색보법측정삼칠접골환중작약감적함량。방법채용 Kromasil 100- A C18（4.6 mm ×250 mm，5μm）색보주，류동상위을정-0.1%린산용액（14：86），주온30℃，검측파장230 nm。결과작약감진양량재0.1182~1.1820μg 범위내정량호적선성관계，평균가양회수솔위99.12%，RSD 위0.66%。결론해방법조작간편，중현성호，결과준학，가용우삼칠접골환중작약감적함량측정。
Objective To establish a RP - HPLC method for the determination of paeoniflorin in Sanqi Jiegu Pills. Methods The method was performed on a Kromasil 100 - A C18(4. 6 mm × 250 mm,5 μm)column with acetonitrile- 0. 1% phosphoric acid(12:88). The column temperature was 30 ℃ . The detection wavelength was 230 nm. Results There was a good linear relationship between the absorption area and the concentration in the range of 0. 118 2 ~ 1. 182 0 μg for paeoniflorin. The average recovery of standard addition were 99. 12% ,RSD = 0. 66%(n = 6). Conclusion This meth-od was simple,accurate and can be used for the quality control of Sanqi Jiegu Pills.
