药学研究
藥學研究
약학연구
Journal of Pharmaceutical Research
2015年
10期
577-578,590
,共3页
银黄解毒胶囊%蒙花苷%高效液相色谱法
銀黃解毒膠囊%矇花苷%高效液相色譜法
은황해독효낭%몽화감%고효액상색보법
Yinhuangjiedu Capsules%Linarin%HPLC
目的:建立高效液相色谱法测定银黄解毒胶囊中蒙花苷的含量。方法采用高效液相色谱法,十八烷基硅烷键合硅胶色谱柱;流动相:甲醇-水-冰醋酸(26:23:1);紫外检测器检测。结果在8.08~56.56μg 范围内蒙花苷峰面积与进样量线性关系良好,r =0.9998;样品加样回收率为99.40%(n =6),RSD 为0.92%。结论所建立的方法准确可行,重复性好,可有效控制银黄解毒胶囊的质量。
目的:建立高效液相色譜法測定銀黃解毒膠囊中矇花苷的含量。方法採用高效液相色譜法,十八烷基硅烷鍵閤硅膠色譜柱;流動相:甲醇-水-冰醋痠(26:23:1);紫外檢測器檢測。結果在8.08~56.56μg 範圍內矇花苷峰麵積與進樣量線性關繫良好,r =0.9998;樣品加樣迴收率為99.40%(n =6),RSD 為0.92%。結論所建立的方法準確可行,重複性好,可有效控製銀黃解毒膠囊的質量。
목적:건립고효액상색보법측정은황해독효낭중몽화감적함량。방법채용고효액상색보법,십팔완기규완건합규효색보주;류동상:갑순-수-빙작산(26:23:1);자외검측기검측。결과재8.08~56.56μg 범위내몽화감봉면적여진양량선성관계량호,r =0.9998;양품가양회수솔위99.40%(n =6),RSD 위0.92%。결론소건립적방법준학가행,중복성호,가유효공제은황해독효낭적질량。
Objective To establish an HPLC method for the determination of linarin in Yinhuangjiedu Capsules. Methods HPLC method was adopted with eighteen alkyl silane bonded silica gel column. Mobile phase was methanol -water - glacial acetic acid(26:23:1),and ultraviolet detector were used. Results There was a good linear relationship be-tween the peak area of linarin and sample in the range of 8. 08 ~ 56. 56,r = 0. 999 8,and the recovery of sample was 99. 40%(n = 6),RSD = 0. 92% . Conclusion The method was feasible,reproducible,and can effectively control the qual-ity of Yinhuangjiedu Capsules.