药学研究
藥學研究
약학연구
Journal of Pharmaceutical Research
2015年
10期
571-573,598
,共4页
六味地黄丸%马钱苷%药代动力学%液质联用
六味地黃汍%馬錢苷%藥代動力學%液質聯用
륙미지황환%마전감%약대동역학%액질련용
Liuwei Dihuang Pills%Loganin%Pharmacokinetics%HPLC - MS
目的：建立液质联用法测定大鼠血浆中马钱苷浓度的方法，研究健康大鼠灌胃六味地黄丸后马钱苷的药代动力学特征。方法6只健康雄性 Wistar 大鼠，分别按10 g·kg -1灌胃给予六味地黄丸混悬液，按设定时间点采集血液样本，采用已建立液质联用法测定血浆中马钱苷浓度。采用 DAS 2.0药代动力学软件计算药代动力学参数。结果马钱苷主要药代动力学参数分别为 t1／2（4.398±1.633）h，Tmax（2.083±1.021）h，Cmax（369.314±53.452）ng·mL -1，AUC0~24（2275.174±553.825）ng·mL -1·h，AUC0~∞（2343.478±601.313）ng·mL -1·h。结论本研究建立的测定大鼠血浆中马钱苷浓度的液质联用法灵敏度高，重现性好，适于大鼠体内马钱苷血浓度测定及药代动力学研究。大鼠灌胃给予六味地黄丸后，马钱苷药时曲线呈双峰。
目的：建立液質聯用法測定大鼠血漿中馬錢苷濃度的方法，研究健康大鼠灌胃六味地黃汍後馬錢苷的藥代動力學特徵。方法6隻健康雄性 Wistar 大鼠，分彆按10 g·kg -1灌胃給予六味地黃汍混懸液，按設定時間點採集血液樣本，採用已建立液質聯用法測定血漿中馬錢苷濃度。採用 DAS 2.0藥代動力學軟件計算藥代動力學參數。結果馬錢苷主要藥代動力學參數分彆為 t1／2（4.398±1.633）h，Tmax（2.083±1.021）h，Cmax（369.314±53.452）ng·mL -1，AUC0~24（2275.174±553.825）ng·mL -1·h，AUC0~∞（2343.478±601.313）ng·mL -1·h。結論本研究建立的測定大鼠血漿中馬錢苷濃度的液質聯用法靈敏度高，重現性好，適于大鼠體內馬錢苷血濃度測定及藥代動力學研究。大鼠灌胃給予六味地黃汍後，馬錢苷藥時麯線呈雙峰。
목적：건립액질련용법측정대서혈장중마전감농도적방법，연구건강대서관위륙미지황환후마전감적약대동역학특정。방법6지건강웅성 Wistar 대서，분별안10 g·kg -1관위급여륙미지황환혼현액，안설정시간점채집혈액양본，채용이건립액질련용법측정혈장중마전감농도。채용 DAS 2.0약대동역학연건계산약대동역학삼수。결과마전감주요약대동역학삼수분별위 t1／2（4.398±1.633）h，Tmax（2.083±1.021）h，Cmax（369.314±53.452）ng·mL -1，AUC0~24（2275.174±553.825）ng·mL -1·h，AUC0~∞（2343.478±601.313）ng·mL -1·h。결론본연구건립적측정대서혈장중마전감농도적액질련용법령민도고，중현성호，괄우대서체내마전감혈농도측정급약대동역학연구。대서관위급여륙미지황환후，마전감약시곡선정쌍봉。
Objective To establish an HPLC - MS methods for the determination of loganin in rats plasma and to study the pharmacokinetic of loganin after administration of Liuwei Dihuang Pills. Methods 6 Wistar rats were administrated with Liuwei Dihuang Pills suspension(10 g·kg - 1 ). The blood samples were collected as scheduled time and analyzed by HPLC - MS. The pharmacokinetic parameters of loganin were calculated by DAS 2. 0 pharmacokinetic software. Results The main parameters of loganin in Liuwei Dihuang Pills were shown as follows:t1 / 2(4. 398 ± 1. 633)h,Tmax(2. 083 ± 1. 021)h,Cmax(369. 314 ± 53. 452)ng·mL - 1 ,AUC0 ~ 24(2 275. 174 ± 553. 825)ng·mL - 1 ·h,AUC0 ~ ∞(2 343 ± 601. 313)ng·mL - 1 ·h. Conclusion The HPLC - MS method was sensitive and selective for determination of loganin in plasma. A bimodal phenomenon was observed in the plasma prole after administration of Liuwei Dihuang Pills.
