CAJ | 학술논문

目的:建立胃灵颗粒的质量标准. 方法:采用TLC法对胃灵颗粒中的主要药味白芍、甘草、延胡索及山楂进行定性鉴别;采用HPLC法测定白芍中芍药苷的含量,色谱柱:Agilent XDB-C18(250 mm × 4. 6 mm,5 μm),流动相:乙腈-0. 1%磷酸溶液(10: 90),检测波长:230 nm,流速:1. 0 ml·min-1. 结果:薄层色谱斑点清晰,在与对照药材或对照品相应的位置上显相同颜色的斑点,阴性对照无干扰;白芍中芍药苷的线性范围0. 151 ~1. 212 μg,r =0. 999 9,平均回收率为99. 63%,RSD 为2. 01%(n=9). 结论:本方法操作简单,结果准确可靠,重复性好,可作为胃灵颗粒的质量控制方法.
목적:건립위령과립적질량표준. 방법:채용TLC법대위령과립중적주요약미백작、감초、연호색급산사진행정성감별;채용HPLC법측정백작중작약감적함량,색보주:Agilent XDB-C18(250 mm × 4. 6 mm,5 μm),류동상:을정-0. 1%린산용액(10: 90),검측파장:230 nm,류속:1. 0 ml·min-1. 결과:박층색보반점청석,재여대조약재혹대조품상응적위치상현상동안색적반점,음성대조무간우;백작중작약감적선성범위0. 151 ~1. 212 μg,r =0. 999 9,평균회수솔위99. 63%,RSD 위2. 01%(n=9). 결론:본방법조작간단,결과준학가고,중복성호,가작위위령과립적질량공제방법.
Objective:To re-establish the quality control standard for Weiling granules. Methods:The 4 chief herbs in the prepa-ration, radices paeoniae alba, licorice, rhizoma corydalis and hawthorn were identified by TLC qualitatively. The content of paeoniflor-in in radices paeoniae alba was determined by HPLC. The separation was performed on an Agilent XDB-C18 (250 mm × 4. 6 mm, 5μm)coulme with mobile phase consisting of acetonitrile-0. 1% phosphoric acid solution (10:90). The detection wavelength was 230 nm and the flow rate was 1. 0 ml·min-1 . Results:The spots in TLC were clear without any interference. The linear range for paeoni-florin was 0. 151-1. 212 μg(r=0. 999 9,n=5). The average recovery was 99. 63% and RSD was 2. 01%(n=9). Conclusion:The method is simple and accurate with high reproducibility, which can be used for the quality control of Weiling granules.