CAJ | 학술논문

目的:通过观察地榆鞣质提取物(STE)对生长转化因子(TGF-β1)诱导人肾小管上皮细胞(HK-2)增殖的影响,探讨地榆在防治肾间质纤维化方面的作用. 方法:将HK-2细胞用含10%胎牛血清的DMEM高糖培养基培养,实验分为5组:空白对照组、TGF-β1 组(5 ng· ml-1 TGF-β1 )、干预1组(5 ng· ml-1 TGF-β1 +12. 5μg·ml-1 STE)、干预2组(5 ng· ml-1 TGF-β1 +25μg·ml-1 STE)、干预3组(5 ng·ml-1 TGF-β1 +50μg·ml-1 STE),孵育24 h. 在倒置显微镜下观察各组细胞形态的改变,并通过CCK8法测定地榆鞣质对细胞增殖的影响. 结果: TGF-β1 5 ng · ml-1能显著诱导人肾小管上皮细胞增殖,并促进其细胞形态向纤维化转变,相比于空白对照组具有显著差异(P<0. 05);但与地榆鞣质提取物(STE)共同作用后,其促进细胞增殖的作用受到一定抑制(P<0. 05),细胞形态也趋于正常,且呈剂量依赖性. 结论:地榆鞣质提取物能抑制HK-2细胞的增殖,在一定程度上具有防止肾间质纤维化的作用.
목적:통과관찰지유유질제취물(STE)대생장전화인자(TGF-β1)유도인신소관상피세포(HK-2)증식적영향,탐토지유재방치신간질섬유화방면적작용. 방법:장HK-2세포용함10%태우혈청적DMEM고당배양기배양,실험분위5조:공백대조조、TGF-β1 조(5 ng· ml-1 TGF-β1 )、간예1조(5 ng· ml-1 TGF-β1 +12. 5μg·ml-1 STE)、간예2조(5 ng· ml-1 TGF-β1 +25μg·ml-1 STE)、간예3조(5 ng·ml-1 TGF-β1 +50μg·ml-1 STE),부육24 h. 재도치현미경하관찰각조세포형태적개변,병통과CCK8법측정지유유질대세포증식적영향. 결과: TGF-β1 5 ng · ml-1능현저유도인신소관상피세포증식,병촉진기세포형태향섬유화전변,상비우공백대조조구유현저차이(P<0. 05);단여지유유질제취물(STE)공동작용후,기촉진세포증식적작용수도일정억제(P<0. 05),세포형태야추우정상,차정제량의뢰성. 결론:지유유질제취물능억제HK-2세포적증식,재일정정도상구유방지신간질섬유화적작용.
Objective:To study the preventive effect of Sanguisorba officinalis on renal fibrosis by observing the influence of San-guisorba officinalis tannins extract (STE) on the proliferation of human renal epithelia (HK-2) induced by transforming growth factor-β1 ( TGF-β1 ) . Methods:HK-2 cells were cultured in DMEM medium with high glucose containing 10% fetal bovine serum. The cul-tured cells were divided into 5 groups, including the blank control group, TGF-β1 group (5 ng· ml-1 TGF-β1), intervention group 1 (5 ng·ml-1 TGF-β1 +12. 5μg·ml-1 STE), intervention group 2 (5 ng·ml-1 TGF-β1 +25μg·ml-1 STE) and intervention group 3(5 ng·ml-1 TGF-β1 +50 μg·ml-1 STE). The changes of cell morphology were observed under an inverted microscope and the in-fluence of SET on the cell proliferation was detected by CCK8 assay. Results: TGF-β1 could significantly induce the proliferation of HK-2 and promote the cell fibrosis with significant difference when compared with the control group (P<0. 05). However, after trea-ted with STE, the cell proliferation was inhibited obviously (P<0. 05) and the cells morphology tended to be normal in a dose-depend-ent manner. Conclusion:STE can inhibit the proliferation of HK-2 and prevent renal fibrosis to some extent.