CAJ | 학술논문

目的：探讨芝麻素对缺血再灌所致大鼠心肌损伤的保护作用及其可能机制。方法：SD大鼠50只随机分为正常对照组、模型组、假手术组、芝麻素低剂量组[80 mg／（kg? d）]及芝麻素高剂量组[160 mg／（kg? d）]，每组10只。芝麻素组灌胃给予芝麻素7 d，每日1次，结扎冠状动脉左前降支建立心肌缺血再灌模型；假手术组大鼠只穿线，不接扎。 HE染色观察心肌病理学变化，比色法测定血清肌酸激酶（ CK）、乳酸脱氢酶（ LDH）、丙二醛（ MDA）、超氧化物歧化酶（ SOD）水平及心肌组织MDA、SOD水平。结果：芝麻素明显改善缺血再灌所致大鼠心肌损伤，减轻细胞水肿、变性、坏死及心肌间质炎症细胞浸润，降低血清CK、LDH水平（P＜0．05或P＜0．01）。芝麻素显著提高大鼠血清和心肌组织SOD活力，降低MDA水平（P＜0．05或P＜0．01），改善缺血再灌诱导的氧化应激。结论：芝麻素可明显改善缺血再灌所致大鼠心肌损伤，其机制与提高机体抗氧化能力、减轻缺血再灌所致氧化应激损伤有关。
목적：탐토지마소대결혈재관소치대서심기손상적보호작용급기가능궤제。방법：SD대서50지수궤분위정상대조조、모형조、가수술조、지마소저제량조[80 mg／（kg? d）]급지마소고제량조[160 mg／（kg? d）]，매조10지。지마소조관위급여지마소7 d，매일1차，결찰관상동맥좌전강지건립심기결혈재관모형；가수술조대서지천선，불접찰。 HE염색관찰심기병이학변화，비색법측정혈청기산격매（ CK）、유산탈경매（ LDH）、병이철（ MDA）、초양화물기화매（ SOD）수평급심기조직MDA、SOD수평。결과：지마소명현개선결혈재관소치대서심기손상，감경세포수종、변성、배사급심기간질염증세포침윤，강저혈청CK、LDH수평（P＜0．05혹P＜0．01）。지마소현저제고대서혈청화심기조직SOD활력，강저MDA수평（P＜0．05혹P＜0．01），개선결혈재관유도적양화응격。결론：지마소가명현개선결혈재관소치대서심기손상，기궤제여제고궤체항양화능력、감경결혈재관소치양화응격손상유관。
Objective:To investigate the protective effect of sesamin and its potential mechanisms against myocardial ischemia reperfusion injury in rats . Methods:Fifty SD rats were randomly divided into 5 groups:namely control group,model group,sham group,low dose of sesamin group [80 mg/(kg? d)] and high dose of sesamin group [80 mg/(kg? d)].Rats in sesamin groups were intragastrically administered with sesamin for 7 d(qd).Then all rats ex-cept those in control and sham groups were subjected to myocardial ischemia for 40 min by coronary artery ligation and subsequent reperfusion for 120 min. Rats in the sham group underwent the same operation without occlusion .Pathological changes of myocardial tissue were examined by hemotoxylin and eosin (HE) staining.Colorimetric assay was performed to determine the serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH),oxidative stress indexes including superoxide dismutase (SOD) activity and malondyaldehyde (MDA) content in serum and myocardial tissues.Results:Pretreat-ment with sesamin evidently ameliorated myocardial injury induced by ischemia and reperfusion in rats as confirmed by histopathological examination and reduced serum levels of LDH and CK activity (P<0.05 or P<0.01).Supplementation with sesamin resulted in a significant increase of SOD activity and decrease of MDA content in serum and myocardial tissues (P<0.05 or P<0.01).Conclusion:Sesamin effectively attenuated myocardial ischemia reper-fusion injury in rats,of which the underlying mechanisms might involve enhancement of antioxidant capacity .