CAJ | 학술논문

以陇东地区子午岭掌叶大黄为实验材料，采用均匀设计法设计试验，以各成分的 Rf 值为评价指标，分析比较了各展开体系对掌叶大黄总蒽醌类成分的展开情况。结果表明：基于均匀设计法进行的12种展开系统中，以丙酮∶甲醇∶正己烷∶1，2-二氯乙烷＝1∶1∶1∶1展开体系效果最佳，展开斑点个数达9个，且各 Rf值间差异极显著（ P＜0．01）。本研究采用均匀设计法优选了大黄总蒽醌类成分的薄层层析展开剂，与药典记录的游离蒽醌展开剂相比，具有样品预处理简单、节约有机溶剂等优越性，可为大孔吸附树脂法进一步分离蒽醌类物质的定性分析提供数据参考。
이롱동지구자오령장협대황위실험재료，채용균균설계법설계시험，이각성분적 Rf 치위평개지표，분석비교료각전개체계대장협대황총은곤류성분적전개정황。결과표명：기우균균설계법진행적12충전개계통중，이병동∶갑순∶정기완∶1，2-이록을완＝1∶1∶1∶1전개체계효과최가，전개반점개수체9개，차각 Rf치간차이겁현저（ P＜0．01）。본연구채용균균설계법우선료대황총은곤류성분적박층층석전개제，여약전기록적유리은곤전개제상비，구유양품예처리간단、절약유궤용제등우월성，가위대공흡부수지법진일보분리은곤류물질적정성분석제공수거삼고。
Taking the Rheum palmatclm collected from the Ziwuling region as the experimental materials and taking the developing situation ( Rf value) of its total anthraquinones constituents into consideration , analyzes the different developing solvent systems through uniform design method .The results show that the optimal developing solvent systems are as follows: acetone to methanol to n-hexane to 1 ,2-dichloro-ethane equals 1∶1∶1∶1 ,which could demonstrate 9 different spots on the chromatoplate ,and their Rf val-ues are different significantly ( P <0.01 ) .Compared with developing solvent systems recorded in the Pharmacopoeia of the People ’ s Republic of China ,the optimal the total anthraquinones substances develo-ping solvent system of thin-layer chromatography is selected in this study ,and it demonstrates the advanta-ges including simple sample pretreatment and economizing organic solvent , which would offer the refer-ence data for further study on qualitative analysis of anthraquinone compounds by macroporous adsorption resin separation .