中国药师
中國藥師
중국약사
China Pharmacist
2015年
10期
1652-1656
,共5页
一测多评%相对校正因子%高效液相色谱法%热血宁注射液%芦丁%水仙苷
一測多評%相對校正因子%高效液相色譜法%熱血寧註射液%蘆丁%水仙苷
일측다평%상대교정인자%고효액상색보법%열혈저주사액%호정%수선감
QAMS%Relative correction factor%HPLC%Shuxuening injection%Rutin%Narcissus glycosides
目的:采用一测多评( quantitative analysis of multi-components by single marker,QAMS)法,同时测定舒血宁注射液中6种黄酮类成分,并考察其方法的耐用性与系统适应性. 方法:以舒血宁注射液为研究对象,以芦丁为内参物,建立芦丁与水仙苷、槲皮素-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、山奈酚-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、槲皮素-3-O-[2-O-(1-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷、山奈酚-3-O-[2-O-(6-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷间的相对校正因子( f) ,比较该方法与外标法测定结果的差异,并对该方法进行再验证. 结果:利用相对校正因子计算舒血宁注射液中这6种黄酮类成分的含量,与外标法比较,差异较小( RSD<5%) . 结论:QAMS 可以作为一种简便准确的质量评价模式用于舒血宁注射液多种成分的定量测定,为后续质量标准评价研究提供了参考依据.
目的:採用一測多評( quantitative analysis of multi-components by single marker,QAMS)法,同時測定舒血寧註射液中6種黃酮類成分,併攷察其方法的耐用性與繫統適應性. 方法:以舒血寧註射液為研究對象,以蘆丁為內參物,建立蘆丁與水仙苷、槲皮素-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、山奈酚-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、槲皮素-3-O-[2-O-(1-O)-對香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷、山奈酚-3-O-[2-O-(6-O)-對香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷間的相對校正因子( f) ,比較該方法與外標法測定結果的差異,併對該方法進行再驗證. 結果:利用相對校正因子計算舒血寧註射液中這6種黃酮類成分的含量,與外標法比較,差異較小( RSD<5%) . 結論:QAMS 可以作為一種簡便準確的質量評價模式用于舒血寧註射液多種成分的定量測定,為後續質量標準評價研究提供瞭參攷依據.
목적:채용일측다평( quantitative analysis of multi-components by single marker,QAMS)법,동시측정서혈저주사액중6충황동류성분,병고찰기방법적내용성여계통괄응성. 방법:이서혈저주사액위연구대상,이호정위내삼물,건립호정여수선감、곡피소-3-O-β-D-포도당기(1-2)-α-L-서리당감、산내분-3-O-β-D-포도당기(1-2)-α-L-서리당감、곡피소-3-O-[2-O-(1-O)-대향두선기-β-D-포도당기]-α-L-서리당감、산내분-3-O-[2-O-(6-O)-대향두선기-β-D-포도당기]-α-L-서리당감간적상대교정인자( f) ,비교해방법여외표법측정결과적차이,병대해방법진행재험증. 결과:이용상대교정인자계산서혈저주사액중저6충황동류성분적함량,여외표법비교,차이교소( RSD<5%) . 결론:QAMS 가이작위일충간편준학적질량평개모식용우서혈저주사액다충성분적정량측정,위후속질량표준평개연구제공료삼고의거.
Objective:To simultaneous determine six flavonoids in Shuxuening injection by quantitative analysis of multi-compo-nents by single marker ( QAMS) and examine the durability and system suitability of the analysis. Methods:Shuxuening injection was the study object and rutin was used as the internal material, the relative correction factor ( f) between rutin and narcissus glycosides, quercetin-3-O-β-D-glucosyl (1-2)-α-L- rhamnoside, kaempferol-3-O-β-D-glucose group (1-2)-α-L-rhamnopyranoside, quercetin-3-O-[2-O-(1-O)-p-coumaric acid-β-D-glucose-yl]-α-L-rhamnoside, kaempferol-3-O-[2-O-(6-O)-on coumaroyl-β-D-glucose-based]-α-L-rhamnosides was respectively established. The result differences between the internal standard method and external standard method were compared, and the internal standard method was re-validated. Results: The contents of the six flavonoids in Shuxuening injection were calculated by the correction factors. Compared with those of the external standard method, the differences in the contents of the method were small( RSD<5%) . Conclusion:QAMS can be used as a simple and accurate quantitative quality evaluation meth-od for the determination of a variety of ingredients in Shuxuening injection, which provides reference for the subsequent study on quality standard.
