CAJ | 학술논문

目的:建立HIV-1病毒感染因子Vif抑制药VEC-5脂质体中VEC-5含量及包封率的HPLC检测方法. 方法:采用冻干重建法制备VEC-5脂质体,超速离心法分离游离药物与脂质体, HPLC法测定脂质体中 VEC-5 的含量及包封率. 结果:VEC-5在20~100 μg·ml-1范围内线性关系良好(r=0. 999 0). 平均回收率为100. 25%,RSD为0. 93%(n=9). 检测三批VEC-5脂质体样品的含量分别为98. 63%、100. 43%、102. 65%,均在标示量的90% ~110%之间;包封率分别为94. 89%、93. 68%、94. 56%,均在90%以上,符合药典关于脂质体制剂包封率大于80% 的要求. 结论:该方法准确、可靠,可用于VEC-5脂质体中VEC-5含量及包封率的测定.
목적:건립HIV-1병독감염인자Vif억제약VEC-5지질체중VEC-5함량급포봉솔적HPLC검측방법. 방법:채용동간중건법제비VEC-5지질체,초속리심법분리유리약물여지질체, HPLC법측정지질체중 VEC-5 적함량급포봉솔. 결과:VEC-5재20~100 μg·ml-1범위내선성관계량호(r=0. 999 0). 평균회수솔위100. 25%,RSD위0. 93%(n=9). 검측삼비VEC-5지질체양품적함량분별위98. 63%、100. 43%、102. 65%,균재표시량적90% ~110%지간;포봉솔분별위94. 89%、93. 68%、94. 56%,균재90%이상,부합약전관우지질체제제포봉솔대우80% 적요구. 결론:해방법준학、가고,가용우VEC-5지질체중VEC-5함량급포봉솔적측정.
Objective:To establish an HPLC method for the determination of content and entrapment efficiency of HIV-1 virus in-fection factor Vif inhibitor VEC-5 liposomes. Methods:VEC-5 liposomes were prepared by a method of freeze-drying and reconstruc-tion. The separation of free drug from the liposomes was achieved by ultracentrifugation, and an HPLC method was used to determine the content and entrapment efficiency of VEC-5 liposomes. Results:The linear range of VEC-5 was 20-100 μg·ml-1(r=0. 999 0). The average recovery was 100. 25% and RSD was 0. 93%(n=9). The content of three batches of VEC-5 liposomes was 98. 63%, 100. 43% and 102. 65%, respectively within the range of 90%-110%, and the entrapment efficiency was 94. 89%, 93. 68% and 94. 56%, respectively, which was above 90%. Conclusion:The method is accurate and reliable, which can be used to determine the content and entrapment efficiency of VEC-5 liposomes.