福建稻麦科技
福建稻麥科技
복건도맥과기
Fujian Science and Technology of Rice and Wheat
2015年
3期
4-7,8
,共5页
王运斌%江良荣%黄荣裕%黄育民%郑景生
王運斌%江良榮%黃榮裕%黃育民%鄭景生
왕운빈%강량영%황영유%황육민%정경생
非变性聚丙烯酰胺凝胶%电泳%银染
非變性聚丙烯酰胺凝膠%電泳%銀染
비변성취병희선알응효%전영%은염
non-denaturant polyacrylamide gel%electrophoresis%silver staining
非变性聚丙烯酰胺凝胶电泳银染技术已广泛用于DNA片段检测。介绍一种本实验室改进的高效省本的非变性聚丙烯酰胺凝胶电泳银染方法，该法将固定和染色两步骤合并，整个银染过程只需16min即可完成。同时，该法无需使用无水乙醇和冰乙酸，减少了硝酸银、氢氧化钠及甲醛的使用量。
非變性聚丙烯酰胺凝膠電泳銀染技術已廣汎用于DNA片段檢測。介紹一種本實驗室改進的高效省本的非變性聚丙烯酰胺凝膠電泳銀染方法，該法將固定和染色兩步驟閤併，整箇銀染過程隻需16min即可完成。同時，該法無需使用無水乙醇和冰乙痠，減少瞭硝痠銀、氫氧化鈉及甲醛的使用量。
비변성취병희선알응효전영은염기술이엄범용우DNA편단검측。개소일충본실험실개진적고효성본적비변성취병희선알응효전영은염방법，해법장고정화염색량보취합병，정개은염과정지수16min즉가완성。동시，해법무수사용무수을순화빙을산，감소료초산은、경양화납급갑철적사용량。
A method of silver staining in non-denaturant polyacrylamide gel electrophoresis has widely been used to detect DNA fragments. Here we developed a high-efficiency and low cost method of DNA silver staining in non-denaturant polyacrylamide gel electrophoresis. The whole new procedure of silver staining has combined two steps of fixation and staining into one step and can be completed within 16 min. Furthermore, the new procedure eliminates the use of ethanol and acetic acid and reduces the use amount of silver nitrate, sodium hydroxide and formaldehyde in silver steps.
