林业科学
林業科學
임업과학
Scientia Silvae Sinicae
2015年
9期
35-41
,共7页
李丹%黄绢%张伟溪%丁昌俊%苏晓华%黄秦军
李丹%黃絹%張偉溪%丁昌俊%囌曉華%黃秦軍
리단%황견%장위계%정창준%소효화%황진군
库安托杨%JERF36%离子流%耐盐性
庫安託楊%JERF36%離子流%耐鹽性
고안탁양%JERF36%리자류%내염성
Populus × euramericana‘Guariento’%JERF36%ion fluxes%salt tolerance
【目的】细胞质内 K +/Na +平衡对细胞的代谢起重要作用,同时也被认为是植物抵御盐胁迫的重要部分。【方法】以转多基因库安托杨株系 D5-21、D5-20及非转基因受体 D5-0为材料,研究100 mmol·L -1 NaCl处理的不同时期(7,15,30天)各杨树株系的株高、根部干物质量、根尖离子流( Na +、K +、H +)的动态变化(利用非损伤微测技术)。【结果】生长数据表明:与正常供水条件相比,7天盐胁迫处理后,3个株系的株高降低,但不显著;15天处理后,D5-20,D5-0株高显著降低;30天处理后,3个株系株高和根部干物质量均显著降低,D5-0,D5-21的根干物质量只有正常供水条件下的一半,说明盐胁迫严重抑制杨树根的生长;但表型变化表明盐胁迫下D5-21, D5-20植株的受害程度更小,并且株高和根部干物质量均高于 D5-0。Na +流测定结果表明:7天盐胁迫处理后,各株系根尖的 Na +流虽与对照相比有显著变化,但变化幅度均较小;15天盐胁迫处理后,D5-21,D5-20根尖 Na +外排能力均是 D5-0的1.8倍;30天处理后,D5-21,D5-20根尖 Na +外排能力均是 D5-0的1.6倍。K +流测定结果表明:D5-0,D5-21根尖 K +外流流速随着胁迫时间延长而增大,D5-20则是先增大后减小,但3个株系变化趋势差异较大,30天处理时,D5-0的流速已是D5-21,D5-20的1.7倍和2.1倍。H +流测定结果表明:虽然与 D5-21, D5-20一样,D5-0的 H +内流值随着胁迫时间延长而增大,但 D5-21,D5-20的增幅明显大于D5-0,30天处理时,二者的 H +流速是 D5-0的1.6倍和1.2倍。因此,不论是短期(7天)、中期(15天)还是长期(30天)盐胁迫处理,与非转基因株系相比,转基因株系 D5-21,D5-20都具有更强的 Na +外排和 H +内流能力,且 K +的外流幅度远低于D5-0,说明转基因株系可通过将根尖细胞质中过多的 Na +主动外排、并减少根尖细胞中 K +的损失,维持胞质中的适宜的 K +/Na +,转基因株系的耐盐性提高,从而有利于转基因株系的生长积累,这将为转基因林木的耐盐性筛选提供新的参考依据。【结论】JERF36基因属于 ERF类转录因子,它可激活植物中下游抗逆相关基因的表达,与植物的抗逆性密切相关,因此未来可研究盐胁迫下转多基因库安托杨中 JERF36基因与 Na +,K +,H +这3种重要离子的转运所涉及的相关基因表达的关系。
【目的】細胞質內 K +/Na +平衡對細胞的代謝起重要作用,同時也被認為是植物牴禦鹽脅迫的重要部分。【方法】以轉多基因庫安託楊株繫 D5-21、D5-20及非轉基因受體 D5-0為材料,研究100 mmol·L -1 NaCl處理的不同時期(7,15,30天)各楊樹株繫的株高、根部榦物質量、根尖離子流( Na +、K +、H +)的動態變化(利用非損傷微測技術)。【結果】生長數據錶明:與正常供水條件相比,7天鹽脅迫處理後,3箇株繫的株高降低,但不顯著;15天處理後,D5-20,D5-0株高顯著降低;30天處理後,3箇株繫株高和根部榦物質量均顯著降低,D5-0,D5-21的根榦物質量隻有正常供水條件下的一半,說明鹽脅迫嚴重抑製楊樹根的生長;但錶型變化錶明鹽脅迫下D5-21, D5-20植株的受害程度更小,併且株高和根部榦物質量均高于 D5-0。Na +流測定結果錶明:7天鹽脅迫處理後,各株繫根尖的 Na +流雖與對照相比有顯著變化,但變化幅度均較小;15天鹽脅迫處理後,D5-21,D5-20根尖 Na +外排能力均是 D5-0的1.8倍;30天處理後,D5-21,D5-20根尖 Na +外排能力均是 D5-0的1.6倍。K +流測定結果錶明:D5-0,D5-21根尖 K +外流流速隨著脅迫時間延長而增大,D5-20則是先增大後減小,但3箇株繫變化趨勢差異較大,30天處理時,D5-0的流速已是D5-21,D5-20的1.7倍和2.1倍。H +流測定結果錶明:雖然與 D5-21, D5-20一樣,D5-0的 H +內流值隨著脅迫時間延長而增大,但 D5-21,D5-20的增幅明顯大于D5-0,30天處理時,二者的 H +流速是 D5-0的1.6倍和1.2倍。因此,不論是短期(7天)、中期(15天)還是長期(30天)鹽脅迫處理,與非轉基因株繫相比,轉基因株繫 D5-21,D5-20都具有更彊的 Na +外排和 H +內流能力,且 K +的外流幅度遠低于D5-0,說明轉基因株繫可通過將根尖細胞質中過多的 Na +主動外排、併減少根尖細胞中 K +的損失,維持胞質中的適宜的 K +/Na +,轉基因株繫的耐鹽性提高,從而有利于轉基因株繫的生長積纍,這將為轉基因林木的耐鹽性篩選提供新的參攷依據。【結論】JERF36基因屬于 ERF類轉錄因子,它可激活植物中下遊抗逆相關基因的錶達,與植物的抗逆性密切相關,因此未來可研究鹽脅迫下轉多基因庫安託楊中 JERF36基因與 Na +,K +,H +這3種重要離子的轉運所涉及的相關基因錶達的關繫。
【목적】세포질내 K +/Na +평형대세포적대사기중요작용,동시야피인위시식물저어염협박적중요부분。【방법】이전다기인고안탁양주계 D5-21、D5-20급비전기인수체 D5-0위재료,연구100 mmol·L -1 NaCl처리적불동시기(7,15,30천)각양수주계적주고、근부간물질량、근첨리자류( Na +、K +、H +)적동태변화(이용비손상미측기술)。【결과】생장수거표명:여정상공수조건상비,7천염협박처리후,3개주계적주고강저,단불현저;15천처리후,D5-20,D5-0주고현저강저;30천처리후,3개주계주고화근부간물질량균현저강저,D5-0,D5-21적근간물질량지유정상공수조건하적일반,설명염협박엄중억제양수근적생장;단표형변화표명염협박하D5-21, D5-20식주적수해정도경소,병차주고화근부간물질량균고우 D5-0。Na +류측정결과표명:7천염협박처리후,각주계근첨적 Na +류수여대조상비유현저변화,단변화폭도균교소;15천염협박처리후,D5-21,D5-20근첨 Na +외배능력균시 D5-0적1.8배;30천처리후,D5-21,D5-20근첨 Na +외배능력균시 D5-0적1.6배。K +류측정결과표명:D5-0,D5-21근첨 K +외류류속수착협박시간연장이증대,D5-20칙시선증대후감소,단3개주계변화추세차이교대,30천처리시,D5-0적류속이시D5-21,D5-20적1.7배화2.1배。H +류측정결과표명:수연여 D5-21, D5-20일양,D5-0적 H +내류치수착협박시간연장이증대,단 D5-21,D5-20적증폭명현대우D5-0,30천처리시,이자적 H +류속시 D5-0적1.6배화1.2배。인차,불론시단기(7천)、중기(15천)환시장기(30천)염협박처리,여비전기인주계상비,전기인주계 D5-21,D5-20도구유경강적 Na +외배화 H +내류능력,차 K +적외류폭도원저우D5-0,설명전기인주계가통과장근첨세포질중과다적 Na +주동외배、병감소근첨세포중 K +적손실,유지포질중적괄의적 K +/Na +,전기인주계적내염성제고,종이유리우전기인주계적생장적루,저장위전기인림목적내염성사선제공신적삼고의거。【결론】JERF36기인속우 ERF류전록인자,타가격활식물중하유항역상관기인적표체,여식물적항역성밀절상관,인차미래가연구염협박하전다기인고안탁양중 JERF36기인여 Na +,K +,H +저3충중요리자적전운소섭급적상관기인표체적관계。
[Objective]Intracellular K + /Na + homeostasis is crucial for cell metabolism and is considered to be a key component of salinity tolerance in plants.[Method]The plant height,dry biomass of roots and net fluxes of Na +,K +,H +from apex roots ( using non-invasive micro-test technology ) were investigated in the multiple transgenic Populus × euramericana‘Guariento’(named D5-21,D5-20)and non-transgenic species(named D5-0)under 100 mmol·L-1 NaCl stress at three different period. [Result]Growth data indicated that: compared with control,all of the three lines had a lower height after 7 days of salt stress treatment,but no significant declined; But after 15 days treatment,the plant height of D5-20,D5-0 was declined significantly;when the treatment extended to 30 days,the height of three poplar lines and their amount of dry matter in root were significantly reduced,the D5-0 and D5-21 remained only 50% of dry matter compare with normal water supply conditions,these data showed that salt stress inhibited the growth of poplar roots seriously; meanwhile,the phenotypic changes showed that,compare to D5-0,D5-21,D5-20 were under lower danger level,had higher height and more dry matter in root after treatment. Na + fluxes results showed that: there is a significant change of each line roots’Na + fluxes after 7 days of stress treatment,but the magnitude is too small; D5-21,D5-20 roots’Na + extruding capacity is 1. 8 times of D5-0 after 15 days of stress treatment; D5-21,D5-20 roots’Na + extruding capacity is 1. 6 times of D5-0 after 30 days of stress treatment. K + fluxes results showed that as stress time continues, D5-0,D5-21 roots’K +effluxing increased,D5-20 is then decreased,but the trends are quite different,D5-0 roots’K +effluxing capacity is 1. 7 times of D5-21 and 2. 1times of D5-20 after 30 days of stress treatment. H + fluxes results showed: as stress time continues,all the three lines roots’H +influxing increased,but the increasing of D5-21、D5-20 is significantly greater than D5-0; D5-21,D5-20 roots’H + influxing capacity is 1. 6 times and 1. 2 times of D5-0 after 30 days of stress treatment. So,whether it is short-term (7 days) ,medium-term (15 days) or long term (30 days) salt stress treatment,transgenic lines D5-21,D5-20 exhibited a higher capacity to extrude Na +,meanwhile,the influx of H +had a significant increasing,and a less reduction of K +versus D5-0,Thus,the transgenic poplar species can avoid excessive cytoplasmic Na + accumulation by extruding Na +,and decreased the K + leakage from cytoplasmic,and these two measuremtnts are helpful to maintain K + /Na +homeostasis. Therefore,transgenic poplar species implied an increased salt tolerance and growth accumulation,this will provide a new evidence for salt tolerance screening of transgenic trees.[Conclusion]JERF36 gene is a ERF transcription factor that can activates the expression of downstream stress resistance genes,and is closely related with plants resistance. So the relationship between JERF36 gene and the gene expression involving in the transport of Na +,H +,K + ions deserves to take a further study.