湖北民族学院学报（医学版）
湖北民族學院學報（醫學版）
호북민족학원학보（의학판）
Journal of Hubei University for Nationalities (Medical Edition)
2015年
3期
7-9
,共3页
姜黄素%PI3K/AKT信号通路%caspase-3
薑黃素%PI3K/AKT信號通路%caspase-3
강황소%PI3K/AKT신호통로%caspase-3
curcumin%PI3K/AKT signaIing pathway%caspase-3
目的：探讨姜黄素对大鼠脑缺血损伤时脑组织磷脂酰肌醇3激酶（ PI3K）／丝氨酸／苏氨酸蛋白激酶（ AKT）信号转导通路、caspase－3表达的影响及可能机制。方法健康SD雄性大鼠随机分为四组：假手术组、模型组、姜黄素A组和姜黄素B组，每组大鼠18只，免疫组化法检测PI3K、AKT蛋白表达， ELISA法检测caspase－3的含量。干湿重法检测脑组织水含量，甲酰胺法检测血脑屏障通透性。结果模型组比较，姜黄素组PI3K、AKT蛋白均明显增高（P＜0．05，P＜0．01），脑组织含水量、caspase－3及伊文思蓝含量显著降低（P＜0．05，P＜0．01，P＜0．01）。结论姜黄素可能通过激活PI3K／AKT信号通路，下调caspase－3的表达，从而减轻脑缺血性损伤。
目的：探討薑黃素對大鼠腦缺血損傷時腦組織燐脂酰肌醇3激酶（ PI3K）／絲氨痠／囌氨痠蛋白激酶（ AKT）信號轉導通路、caspase－3錶達的影響及可能機製。方法健康SD雄性大鼠隨機分為四組：假手術組、模型組、薑黃素A組和薑黃素B組，每組大鼠18隻，免疫組化法檢測PI3K、AKT蛋白錶達， ELISA法檢測caspase－3的含量。榦濕重法檢測腦組織水含量，甲酰胺法檢測血腦屏障通透性。結果模型組比較，薑黃素組PI3K、AKT蛋白均明顯增高（P＜0．05，P＜0．01），腦組織含水量、caspase－3及伊文思藍含量顯著降低（P＜0．05，P＜0．01，P＜0．01）。結論薑黃素可能通過激活PI3K／AKT信號通路，下調caspase－3的錶達，從而減輕腦缺血性損傷。
목적：탐토강황소대대서뇌결혈손상시뇌조직린지선기순3격매（ PI3K）／사안산／소안산단백격매（ AKT）신호전도통로、caspase－3표체적영향급가능궤제。방법건강SD웅성대서수궤분위사조：가수술조、모형조、강황소A조화강황소B조，매조대서18지，면역조화법검측PI3K、AKT단백표체， ELISA법검측caspase－3적함량。간습중법검측뇌조직수함량，갑선알법검측혈뇌병장통투성。결과모형조비교，강황소조PI3K、AKT단백균명현증고（P＜0．05，P＜0．01），뇌조직함수량、caspase－3급이문사람함량현저강저（P＜0．05，P＜0．01，P＜0．01）。결론강황소가능통과격활PI3K／AKT신호통로，하조caspase－3적표체，종이감경뇌결혈성손상。
Objective To expIore the effects of curcumin on cerebraI ischemia in rat brain PI3K/AKT signaI transduction pathway and the possibIe mechanism.Methods SD rats were randomIy divided into four groups:sham operation group,modeI group,curcumin A group and curcumin B group,18 rats in each group.The rats of modeI group,curcumin A,B group of chronic cerebraI ischemia was prepared by the method of biIateraI carotid artery Iigation in rats,sham operation group and modeI group were given normaI saIine of the same.PI3K, AKT, protein expression were detected by ImmunohistochemicaI method,with the content of Caspase-3 determined by ELISA.The water content of brain tissue was detected by dry wet weight method, the permea-biIity of bIood brain barrier by methanamide method.Results Compared with modeI group,curcu-min group PI3K and AKT protein were significantIy increased (P<0.05,P<0.01),brain water content,caspase-3 and Evans bIue content were significantIy decreased ( P<0.05,P<0.01,P<0.01).Conclusion The possibIe mechanism of the protective effect of curcumin on ischemic cerebraI injury is the penetration of the bIood-brain barrier after the activation of the PI3K/AKT signaIing pathway, and the expression of Caspase-3 mediated by it.
