CAJ | 학술논문

目的：探讨IL－15与舒尼替尼（ Sunitinib ）联合用于抗肿瘤治疗的可行性及抗肿瘤免疫反应的建立机制。方法取健康小鼠外周血单个核细胞（PBMC），预先以常规方法诱导细胞因子诱导的杀伤细胞（CIK），然后加入IL－15和Sunitinib与CIK共培养，设置不加IL－15与Sunitinib组作为对照组。全自动五分类血液分析仪计数 CIK增殖率；ELISA方法检测CIK分泌INF－γ、IL－10和IL－12的水平；51 Cr释放试验检测CIK对B16黑色素瘤细胞的杀伤活性。结果 IL－15联合Sunitinib能显著增强CIK细胞的增殖能力（ P＜0．05）， IFN－γ分泌能力明显高于对照组（P＜0．05），组间IL－10和IL－10的分泌量未见显著差异（P＞0．05），CIK对靶细胞的杀伤活性显著高于对照组（P＜0．05）。结论 CIK培养体系中加入IL－15和Sunitinib可提高CIK抗肿瘤效应，为肿瘤综合治疗提供了新思路。
목적：탐토IL－15여서니체니（ Sunitinib ）연합용우항종류치료적가행성급항종류면역반응적건립궤제。방법취건강소서외주혈단개핵세포（PBMC），예선이상규방법유도세포인자유도적살상세포（CIK），연후가입IL－15화Sunitinib여CIK공배양，설치불가IL－15여Sunitinib조작위대조조。전자동오분류혈액분석의계수 CIK증식솔；ELISA방법검측CIK분비INF－γ、IL－10화IL－12적수평；51 Cr석방시험검측CIK대B16흑색소류세포적살상활성。결과 IL－15연합Sunitinib능현저증강CIK세포적증식능력（ P＜0．05）， IFN－γ분비능력명현고우대조조（P＜0．05），조간IL－10화IL－10적분비량미견현저차이（P＞0．05），CIK대파세포적살상활성현저고우대조조（P＜0．05）。결론 CIK배양체계중가입IL－15화Sunitinib가제고CIK항종류효응，위종류종합치료제공료신사로。
Objective To examine the synergistic effects of IL-15 and sunitinib, and expIore the underIying mechanisms of suc h a sy nergistic effect in immune response.Methods PBMC of heaIthy mice were isoIated and cuItured by the conventionaI method in the pr esent of IL-15 pIus Sunitinib for 7 days.The CIK proIiferation rate was detected by automatic cIassification of five bIood anaIyzer.INF-γ,IL-10 and IL-12 were examined by enzyme-Iinked immuno sorbent assay.Thea nti-tumo r cytotoxicity of CIK was exami ned by 51 Cr reIease assay.Results The com-bined treatment with IL-15 pIus sunitinib maya ugment the proIiferation rate of CI K( P<0.05 ) , and showed more proportions of IFN-γsecreting subsets(P<0.05),the CIK showed a stronger cytotoxic to tumor target ceIIs ( P<0.05 ) .Conclusion Our study suggests that the combined treatm ent with IL-15 pIus sunitinib may augment the anti-tumor cytotoxicity of CIK.The prend-ings may be of importance to the further expIoration of the potentiaI appIication of this com-bined approach in cancer treatment.