CAJ | 학술논문

目的:观察Ihh/Gli1mRNA在脊髓中的分布及大鼠急性脊髓损伤后Ihh/Gli1mRNA信号通路对脊髓内源性干细胞增殖分化的影响.方法:将SD大鼠随机分为空白组(A组)、假手术组(B组)、模型组(C组).大鼠急性脊髓损伤模型用改良Allen's法建立.将大鼠于造模后不同时间点分别处死,取以损伤脊髓为中心长1cm的脊髓进行real-time PCR、原位杂交检测及免疫组化检测.结果:在成年大鼠脊髓中,IhhmRNA在室管膜细胞仅有少量表达,其主要分布在白质区.Gli1mRNA主要表达在灰质区运动神经元核仁以外的细胞核内和胶质细胞额胞质.脊髓损伤后,IhhmRNA和Gli1mRNA表达减少,第3至7天达到最低值后又缓慢增多;Nestin+细胞和Brdu+细胞在第3至7天达到最高值后缓慢下降.结论:脊髓损伤后Ihh/Gli1信号通路对内源性神经干细胞的增殖起负性调控作用.
목적:관찰Ihh/Gli1mRNA재척수중적분포급대서급성척수손상후Ihh/Gli1mRNA신호통로대척수내원성간세포증식분화적영향.방법:장SD대서수궤분위공백조(A조)、가수술조(B조)、모형조(C조).대서급성척수손상모형용개량Allen's법건립.장대서우조모후불동시간점분별처사,취이손상척수위중심장1cm적척수진행real-time PCR、원위잡교검측급면역조화검측.결과:재성년대서척수중,IhhmRNA재실관막세포부유소량표체,기주요분포재백질구.Gli1mRNA주요표체재회질구운동신경원핵인이외적세포핵내화효질세포액포질.척수손상후,IhhmRNA화Gli1mRNA표체감소,제3지7천체도최저치후우완만증다;Nestin+세포화Brdu+세포재제3지7천체도최고치후완만하강.결론:척수손상후Ihh/Gli1신호통로대내원성신경간세포적증식기부성조공작용.
Objective: To observe the distribution and expression of Ihh/ Gli1mRNA in spinal cord of rat after injury and analyze the regulation of Ihh/Gli1 signal transduction pathways on endogenous neural stem cells. Methods: The Sprague Dawley rats were randomly divided into gap group, pseudo surgery group, model group. The rat models of acute spinal cord injury were established by modified Allen's method in the experiment. 6 rats were sacrificed in each group at different time point, and all of the center of upper and lower sections of the 5mm from the injured spinal cords the rats was examined by the in situ hybridization,real-time PCR and immunohistochemistry. Results: Ihh mRNA was mainly distributed in the white matterin of the spinal cord of normal adult rats, and as well as minor distributed in ependymocytes. Gli1mRNA are mainly distributed in the cell nucleolus of gray matter neurons in addition to outside the plasmosome and the cytoplasm of glial cells. After spinal cord injury, the expression of Ihh/ Gli1mRNA was reduced, and there were the lowest expression of Ihh/Gli1mRNA during 3 to 7 days, and then the expression of Ihh mRNA gradually increased. The expression of Nestin+ cell and Brdu+ cell were increased after spinal cord injury, and there were the most expression during 3 to 7 days, and then reduced gradually. Conclusions: Ihh/gli1 transduction pathway regulates the proliferation of endogenous neural stem cells negatively.