现代医药卫生
現代醫藥衛生
현대의약위생
Journal of Modern Medicine & Health
2015年
19期
2892-2895
,共4页
何贵成%严思佳%丁恩%艾小红
何貴成%嚴思佳%丁恩%艾小紅
하귀성%엄사가%정은%애소홍
植物提取物%肝肿瘤/病理学%肿瘤细胞,培养的%8-硝基白杨素%HepG2细胞%分化%诱导
植物提取物%肝腫瘤/病理學%腫瘤細胞,培養的%8-硝基白楊素%HepG2細胞%分化%誘導
식물제취물%간종류/병이학%종류세포,배양적%8-초기백양소%HepG2세포%분화%유도
Plant extracts%Liver neoplasms/pathology%Tumor cells,cultured%8-bromo-7-methoxychrysin(BrMC)%HepG2 cells%Differentiation%Induce
目的：探讨传统中药蜂胶的有效成分白杨素的人工半合成类似物8-溴-7-甲氧基白杨素，即8-硝基白杨素（BrMC）是否具有诱导人肝癌细胞分化作用。方法体外培养人肝癌HepG2细胞，分别加入1.0μg/mL BrMC和1.0μg/mL全反式维甲酸（ATRA），同时设溶媒对照组和空白对照组。瑞氏-姬姆萨染色法检测 BrMC 诱导 HepG2细胞形态与核质比的变化；酶促反应试剂盒检测BrMC 和 ATRA 诱导 HepG2细胞中碱性磷酸酶（ALP）和γ-谷氨酰转肽酶（γ-GT）的活性；放射免疫法检测甲胎蛋白（AFP）的质量分数；Diamondstone分光光度法（速率法）检测酪氨酸转氨酶（TAT）的活性。结果1.0μg/mL BrMC处理的细胞核质比远低于溶媒对照组，差异有统计学意义（P＜0.01）。1.0μg/mL BrMC、ATRA组处理24、48、96 h的HepG2细胞液中AFP质量分数，γ-GT、ALP、TAT活性分别与溶媒对照组比较，差异均有统计学意义（P＜0.01）。结论 BrMC能降低HepG2细胞核质比、减少HepG2细胞的AFP 分泌、有效地活化HepG2细胞TAT、ALP；且BrMC和ATRA均能抑制HepG2细胞γ-GT活性。BrMC通过以上作用诱导人肝癌细胞分化。
目的：探討傳統中藥蜂膠的有效成分白楊素的人工半閤成類似物8-溴-7-甲氧基白楊素，即8-硝基白楊素（BrMC）是否具有誘導人肝癌細胞分化作用。方法體外培養人肝癌HepG2細胞，分彆加入1.0μg/mL BrMC和1.0μg/mL全反式維甲痠（ATRA），同時設溶媒對照組和空白對照組。瑞氏-姬姆薩染色法檢測 BrMC 誘導 HepG2細胞形態與覈質比的變化；酶促反應試劑盒檢測BrMC 和 ATRA 誘導 HepG2細胞中堿性燐痠酶（ALP）和γ-穀氨酰轉肽酶（γ-GT）的活性；放射免疫法檢測甲胎蛋白（AFP）的質量分數；Diamondstone分光光度法（速率法）檢測酪氨痠轉氨酶（TAT）的活性。結果1.0μg/mL BrMC處理的細胞覈質比遠低于溶媒對照組，差異有統計學意義（P＜0.01）。1.0μg/mL BrMC、ATRA組處理24、48、96 h的HepG2細胞液中AFP質量分數，γ-GT、ALP、TAT活性分彆與溶媒對照組比較，差異均有統計學意義（P＜0.01）。結論 BrMC能降低HepG2細胞覈質比、減少HepG2細胞的AFP 分泌、有效地活化HepG2細胞TAT、ALP；且BrMC和ATRA均能抑製HepG2細胞γ-GT活性。BrMC通過以上作用誘導人肝癌細胞分化。
목적：탐토전통중약봉효적유효성분백양소적인공반합성유사물8-추-7-갑양기백양소，즉8-초기백양소（BrMC）시부구유유도인간암세포분화작용。방법체외배양인간암HepG2세포，분별가입1.0μg/mL BrMC화1.0μg/mL전반식유갑산（ATRA），동시설용매대조조화공백대조조。서씨-희모살염색법검측 BrMC 유도 HepG2세포형태여핵질비적변화；매촉반응시제합검측BrMC 화 ATRA 유도 HepG2세포중감성린산매（ALP）화γ-곡안선전태매（γ-GT）적활성；방사면역법검측갑태단백（AFP）적질량분수；Diamondstone분광광도법（속솔법）검측락안산전안매（TAT）적활성。결과1.0μg/mL BrMC처리적세포핵질비원저우용매대조조，차이유통계학의의（P＜0.01）。1.0μg/mL BrMC、ATRA조처리24、48、96 h적HepG2세포액중AFP질량분수，γ-GT、ALP、TAT활성분별여용매대조조비교，차이균유통계학의의（P＜0.01）。결론 BrMC능강저HepG2세포핵질비、감소HepG2세포적AFP 분비、유효지활화HepG2세포TAT、ALP；차BrMC화ATRA균능억제HepG2세포γ-GT활성。BrMC통과이상작용유도인간암세포분화。
Objective To evaluate whether the artificial semisynthetic analogue 8-bromo-7-methoxychrysin (BrMC), i. e., 8-nitrochrysin as an effective component of traditional Chinese medicine propolis,having the ability for inducing the differen-tiation of human hepatocarcinoma cells. Methods The human hepatocarcinoma HepG2 cells were cultured in vitro. The Wright s-Giemse mixed coloring method was adopted to detect the changes of the cellular morphology and the nuclear-cytoplasmic ratio of Hep G2 cells induced by BrMc;the changes of microtubules microfilament protein array of HepG2 cells induced by BrMC and all trans retinoid acid(ATRA) were observed by Coomassie brilliant blue staining;the contents of alkaline phosphatase(ALP) and gamma-glutamyl transpeptidase(γ-GT) in HepG2 cells were detected by the enzyme catalyzed reaction;the radioimmunoassay(RIA) was used to detect the mass fraction of alpha fetal protein(AFP);the content of tyrosine transaminase(TAT) was measured by the Dia-mondstone spectrophotometry. Results The cytoplasmic ratio by 1.0 ug/mL BrMC handled was far lower than that of solvent con trol group,the difference was statistically significant(P<0.01).1.0μg/mL BrMC and ATRA handled 24,48,96 hours,the mass fraction of AFP,activity ofγ-GT,ALP,TAT compared with the solvent control group respectively,the difference were statistically significant(P<0.01). Conclusion BrMC induces human hepatocarcinoma cellular differentiation by lowing the nucleus-cytoplas-mic ratio,decreasing the secretion of AFP,effectively activating TAT and ALP in HepG2 cells;moreover BrMc and ATRA inhibit-ing the activity ofγ-GT.
