CAJ | 학술논문

目的: 建立厚朴配方颗粒厚朴酚与和厚朴酚含量测定方法,比较不同厂家厚朴配方颗粒中厚朴酚及和厚朴酚的含量. 方法:应用HPLC法测定,色谱柱:HypersiL C18 (250 mm × 4. 6 mm,5 μm);流动相:A相为乙腈,B相为水,梯度洗脱;流速:1. 0 ml·min-1 ,柱温:25℃,检测波长:294 nm,进样量:20 μl. 结果:厚朴酚在0. 873~26. 190 μg·ml-1范围内与峰面积呈良好的线性关系(r=0. 999 5),平均回收率为99. 24%,RSD=2. 00%(n=6);和厚朴酚浓度在0. 732~21. 980 μg·ml-1范围内与峰面积呈良好的线性关系(r=0. 999 0),平均回收率99. 89%,RSD=1. 33%(n=6). 不同厂家生产的厚朴配方颗粒中厚朴酚与和厚朴酚含量存在较大差异. 结论:本方法简便、准确、重复性好,可用于厚朴配方颗粒中厚朴酚与和厚朴酚含量测定,不同厂家厚朴配方颗粒的质量差异提示规范药材种植、遴选、制定科学统一的配方颗粒生产工艺和质量控制标准的必要性.
목적: 건립후박배방과립후박분여화후박분함량측정방법,비교불동엄가후박배방과립중후박분급화후박분적함량. 방법:응용HPLC법측정,색보주:HypersiL C18 (250 mm × 4. 6 mm,5 μm);류동상:A상위을정,B상위수,제도세탈;류속:1. 0 ml·min-1 ,주온:25℃,검측파장:294 nm,진양량:20 μl. 결과:후박분재0. 873~26. 190 μg·ml-1범위내여봉면적정량호적선성관계(r=0. 999 5),평균회수솔위99. 24%,RSD=2. 00%(n=6);화후박분농도재0. 732~21. 980 μg·ml-1범위내여봉면적정량호적선성관계(r=0. 999 0),평균회수솔99. 89%,RSD=1. 33%(n=6). 불동엄가생산적후박배방과립중후박분여화후박분함량존재교대차이. 결론:본방법간편、준학、중복성호,가용우후박배방과립중후박분여화후박분함량측정,불동엄가후박배방과립적질량차이제시규범약재충식、린선、제정과학통일적배방과립생산공예화질량공제표준적필요성.
Objective:To establish an HPLC method for the determination of the content of magnolol and honokiol in Cortex mag-noliae Officinalis formula granules and compare the content of the formula granules from different manufacturers. Methods:An HPLC was used to determine the content of magnolol and honokiol in Cortex magnoliae Officinalis formula granules. The analysis was carried out on a Hypersil C18 (250 mm × 4. 6 mm, 5 μm) chromatographic column. Acetonitrile-water was used as the mobile phase with gra-dient elution and the flow rate was 1. 0 ml·min-1 . The detection wavelength was set at 294 nm, the sample size was 20 μl and the column temperature was 25℃. Results:The linear range was 0. 873-26. 190μg·ml-1(r=0. 999 5) for magnolol, the average recov-ery was 99. 24% with RSD of 2. 00%(n=6) and that was 0. 732-21. 980μg·ml-1(r=0. 999 0) for honokiol,and the average recov-ery was 99. 89% with RSD of 1. 33%(n=6). The difference in the content of magnolol and honokiol in Cortex magnoliae Officinalis formula granules from different manufacturers was notable. Conclusion: The method is simple, repeatable and feasible, and can be used for the quality control of magnolol and honokiol in Cortex magnoliae Officinalis formula granules. The content difference in magno-lol and honokiol in Cortex magnoliae Officinalis formula granules from different manufacturers suggests that it is necessary to standardize the planting and selecting of Chinese medicine, and develop scientific and unified production technology and quality standard for the formula granules.