中国药物与临床
中國藥物與臨床
중국약물여림상
Chinese Remedies & Clinics
2015年
9期
1225-1228
,共4页
许召良%马志方%岳亮%王东文
許召良%馬誌方%嶽亮%王東文
허소량%마지방%악량%왕동문
肥大细胞%前列腺肿瘤%上皮间质转化
肥大細胞%前列腺腫瘤%上皮間質轉化
비대세포%전렬선종류%상피간질전화
Mast cells%Prostate neoplasm%Epithelial-mesenchymal transition
目的 利用体外细胞共培养模型,探讨肥大细胞对前列腺癌细胞增殖及侵袭、转移的作用.方法 选取肥大细胞瘤P815细胞系及前列腺癌LNCaP细胞,使用24孔Transwell小室构建2种细胞体外共培养模型.四甲基偶氮唑蓝(MTT)比色试验检测前列腺癌LNCaP细胞的增殖,反转录定量聚合酶链反应(qRT-PCR)方法和蛋白印迹法检测前列腺癌LNCaP细胞E-cad、N-cad、Vimentin的表达.结果 前列腺癌LNCaP细胞与不同浓度肥大细胞共同培养12 h后吸光度(A)值变化与对照组差异无统计学意义(P>0.05),共同培养24 h及48 h后A值显著高于对照组,差异有统计学意义(P<0.05);与对照组比较,实验组E-cad表达明显减弱;N-cad、Vi-mentin表达增强,差异有统计学意义(P<0.05).结论 肥大细胞可促进前列腺癌细胞的增殖并促进前列腺癌细胞的上皮间质转化,可能促进前列腺癌细胞侵袭、转移.
目的 利用體外細胞共培養模型,探討肥大細胞對前列腺癌細胞增殖及侵襲、轉移的作用.方法 選取肥大細胞瘤P815細胞繫及前列腺癌LNCaP細胞,使用24孔Transwell小室構建2種細胞體外共培養模型.四甲基偶氮唑藍(MTT)比色試驗檢測前列腺癌LNCaP細胞的增殖,反轉錄定量聚閤酶鏈反應(qRT-PCR)方法和蛋白印跡法檢測前列腺癌LNCaP細胞E-cad、N-cad、Vimentin的錶達.結果 前列腺癌LNCaP細胞與不同濃度肥大細胞共同培養12 h後吸光度(A)值變化與對照組差異無統計學意義(P>0.05),共同培養24 h及48 h後A值顯著高于對照組,差異有統計學意義(P<0.05);與對照組比較,實驗組E-cad錶達明顯減弱;N-cad、Vi-mentin錶達增彊,差異有統計學意義(P<0.05).結論 肥大細胞可促進前列腺癌細胞的增殖併促進前列腺癌細胞的上皮間質轉化,可能促進前列腺癌細胞侵襲、轉移.
목적 이용체외세포공배양모형,탐토비대세포대전렬선암세포증식급침습、전이적작용.방법 선취비대세포류P815세포계급전렬선암LNCaP세포,사용24공Transwell소실구건2충세포체외공배양모형.사갑기우담서람(MTT)비색시험검측전렬선암LNCaP세포적증식,반전록정량취합매련반응(qRT-PCR)방법화단백인적법검측전렬선암LNCaP세포E-cad、N-cad、Vimentin적표체.결과 전렬선암LNCaP세포여불동농도비대세포공동배양12 h후흡광도(A)치변화여대조조차이무통계학의의(P>0.05),공동배양24 h급48 h후A치현저고우대조조,차이유통계학의의(P<0.05);여대조조비교,실험조E-cad표체명현감약;N-cad、Vi-mentin표체증강,차이유통계학의의(P<0.05).결론 비대세포가촉진전렬선암세포적증식병촉진전렬선암세포적상피간질전화,가능촉진전렬선암세포침습、전이.
Objective To explore the role of mast cells in proliferation, invasion and metastasis of prostate cancer cells by using in-vitro cell co-culture model. Methods In-vitro co-culture model of mastocytoma P815 cell lines and prostate cancer LNCap cells was constructed by using 24-well Transwell chamber. Methylthiazolyl tetrazoli-um blue (MTT) colorimetry was used to detect the proliferation of prostate cancer LNCap cells. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the expression of E-cad, N-cad and Vimentin in prostate cancer LNCap cells. Results Compared with the control group, the absorbance (A value) of prostate cancer LNCap cells co-cultured with different concentrations of mast cells was not significantly dif-ferent at 12h (P>0.05), but was significantly higher at 24h and 48h (P<0.05). The experimental group showed signifi-cantly down-regulated E-cad expression and up-regulated N-cad and Vimentin expression, with significant difference compared with the control group (P<0.05). Conclusion Mast cells can promote the proliferation and the epithelial-mesenchymal transition of prostate cancer cells, and may also promote the invasion and metastasis of prostate cancer cells.
