中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
Journal of Endocrine Surgery
2015年
4期
272-274,279
,共4页
陈国%韩鹏黎%吴迪%霍彦平%吕晶%李明闯%殷德涛
陳國%韓鵬黎%吳迪%霍彥平%呂晶%李明闖%慇德濤
진국%한붕려%오적%곽언평%려정%리명틈%은덕도
微管相关肿瘤抑制基因1%甲状腺乳头状癌%RT-PCR
微管相關腫瘤抑製基因1%甲狀腺乳頭狀癌%RT-PCR
미관상관종류억제기인1%갑상선유두상암%RT-PCR
Mitochondrial tumor suppressor gene 1%Papillary thyroid carcinoma%RT-PCR
目的 探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中微管相关肿瘤抑制基因1(mitochondrial tumor suppressor gene 1,MTUS1)基因mRNA表达情况.方法 采用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)检测68例PTC组织及其对应癌旁正常组织(non-carcerous epithelium,NCE)中MTUS1 mRNA表达.结果 在NCE中MTSU1 mRNA平均灰度值为(0.65±0.12),高于PTC组织中MTSU1 mRNA平均灰度值(0.31±0.09),2组比较差异有统计学意义(t =2.39,P＜0.05).在PTC组织中,MTUS1基因mRNA的表达与患者的病理分级及淋巴结转移有关(P＜0.05),与患者性别、年龄、分化程度、肿瘤大小及TNM分期均无明显关系(P＞0.05).结论 抑癌基因MTUS1在PTC组织中低表达,MTUS1基因的表达异常在PTC的发生、发展中可能起着重要的作用.
目的 探討甲狀腺乳頭狀癌(papillary thyroid carcinoma,PTC)組織中微管相關腫瘤抑製基因1(mitochondrial tumor suppressor gene 1,MTUS1)基因mRNA錶達情況.方法 採用逆轉錄-聚閤酶鏈式反應(reverse transcription-polymerase chain reaction,RT-PCR)檢測68例PTC組織及其對應癌徬正常組織(non-carcerous epithelium,NCE)中MTUS1 mRNA錶達.結果 在NCE中MTSU1 mRNA平均灰度值為(0.65±0.12),高于PTC組織中MTSU1 mRNA平均灰度值(0.31±0.09),2組比較差異有統計學意義(t =2.39,P＜0.05).在PTC組織中,MTUS1基因mRNA的錶達與患者的病理分級及淋巴結轉移有關(P＜0.05),與患者性彆、年齡、分化程度、腫瘤大小及TNM分期均無明顯關繫(P＞0.05).結論 抑癌基因MTUS1在PTC組織中低錶達,MTUS1基因的錶達異常在PTC的髮生、髮展中可能起著重要的作用.
목적 탐토갑상선유두상암(papillary thyroid carcinoma,PTC)조직중미관상관종류억제기인1(mitochondrial tumor suppressor gene 1,MTUS1)기인mRNA표체정황.방법 채용역전록-취합매련식반응(reverse transcription-polymerase chain reaction,RT-PCR)검측68례PTC조직급기대응암방정상조직(non-carcerous epithelium,NCE)중MTUS1 mRNA표체.결과 재NCE중MTSU1 mRNA평균회도치위(0.65±0.12),고우PTC조직중MTSU1 mRNA평균회도치(0.31±0.09),2조비교차이유통계학의의(t =2.39,P＜0.05).재PTC조직중,MTUS1기인mRNA적표체여환자적병리분급급림파결전이유관(P＜0.05),여환자성별、년령、분화정도、종류대소급TNM분기균무명현관계(P＞0.05).결론 억암기인MTUS1재PTC조직중저표체,MTUS1기인적표체이상재PTC적발생、발전중가능기착중요적작용.
Objective To study the expression of mitochondrial tumor suppressor gene 1 (MTUS1) mR-NA in papillary thyroid carcinoma(PTC).Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect MTUS1 mRNA level in 68 cases of human PTC and the adjacent non-cancerous epithelium (NCE) specimens.Results The relative expression value of MTUS1 mRNA in PTC was 0.31 ± 0.09,while it was 0.65 ±0.12 in NCE.The difference had statistical significance between the 2 groups(t =2.39,P ＜0.05).In PTC,mRNA expression of MTUS1 gene was related to the pathological grade,and lymph node metastasis(P ＜ 0.05)while it was not related to patients' age,sex,differentiation degree,tumor size or TNM stage(P ＞ 0.05).Conclusion mRNA expression of MTUS1 gene is lower in PTC than in NCE,which may play an important role in carcinogenesis and progression of PTC.
