CAJ | 학술논문

目的通过检测肥胖相关性肾小球病(ORG)小鼠肾组织中脂氧素A4受体(ALX)mRNA及蛋白的表达,探讨其在ORG发生发展中的作用.方法选取40只35 d龄C57BL/6雄性小鼠,按体质量随机分为ORG组和对照组(各20只),适应性喂养1周后,ORG组高脂高能量饲料喂养,对照组普通饲料喂养,2组分别喂养8周后留取尿液,ELISA法行尿微量系列蛋白检测;游离肾组织,光镜、电镜下观察肾组织病理学改变;qRT?PCR法检测肾组织ALX mRNA表达;Western blot法检测肾组织ALX蛋白表达;结果采用SPSS 17.0软件进行统计学处理.结果与对照组比较,ORG组尿微量蛋白明显增高,肾组织中ALX mRNA及蛋白的表达均明显增强,差异有统计学意义(P<0.05),病理检查显示ORG组肾小球普遍肥大,电镜下见肾小球囊腔增宽,足突部分融合,内皮细胞膜部分模糊,近端小管上皮细胞内见大量空泡变性线粒体.结论 ORG肾组织中ALX表达的增强可能在ORG发生发展过程中发挥调节作用.
목적 통과검측비반상관성신소구병(ORG)소서신조직중지양소A4수체(ALX)mRNA급단백적표체,탐토기재ORG발생발전중적작용.방법 선취40지35 d령C57BL/6웅성소서,안체질량수궤분위ORG조화대조조(각20지),괄응성위양1주후,ORG조고지고능량사료위양,대조조보통사료위양,2조분별위양8주후류취뇨액,ELISA법행뇨미량계렬단백검측;유리신조직,광경、전경하관찰신조직병이학개변;qRT?PCR법검측신조직ALX mRNA표체;Western blot법검측신조직ALX단백표체;결과채용SPSS 17.0연건진행통계학처리.결과 여대조조비교,ORG조뇨미량단백명현증고,신조직중ALX mRNA급단백적표체균명현증강,차이유통계학의의(P<0.05),병리검사현시ORG조신소구보편비대,전경하견신소구낭강증관,족돌부분융합,내피세포막부분모호,근단소관상피세포내견대량공포변성선립체.결론 ORG신조직중ALX표체적증강가능재ORG발생발전과정중발휘조절작용.
Objective To detect the expression of lipoxin A4 receptor(ALX)in renal tissues of mice with obesity?related glomerulopathy(ORG), so as to explore its role and significance in the onset and development of ORG. Methods The clean grade C57BL/6 mice aged 35 days were random?ly divided into ORG group and control group(20 cases in each group). After 1 week of acclimation,the ORG group was fed with a high?fat high?en?ergy diet,while the control group was fed with a normal diet. After 8 weeks feeding,the two groups were assayed with enzyme linked immunosorbent assay(ELISA)for urinary microprotein. The renal tissue was fixed,and examined under both light and electron microscopy for histopathological changes. The quantitative real?time polymerase chain reaction(qRT?PCR)analysis was performed to measure the expression of ALX mRNA in re?nal tissues. ALX protein expression was determined by Western blot assay. The statistical difference between the two groups was analyzed using SPSS 17.0 software. Results Compared with the control group,the ORG group had significantly higher urinary protein;both ALXmRNA and protein ex?pressions in renal tissues of the ORG group were significantly increased(P<0.05). Histopathological examination found the ORG group had general glomerular hypertrophy. Electron microscope results revealed that glomerular cysts widened,part of the foot process fusion,part of the endothelial cell membrane indistinct,and a large number of mitochondria vacuolar degeneration were found in the proximal tubule epithelium. Conclusion In?creased expression of ALX might play a regulated role in the pathogenic mechanism and development of ORG.