口腔生物医学
口腔生物醫學
구강생물의학
Oral Biomedicine
2015年
3期
150-154
,共5页
朱斌%赵喜聪%李楠%金岩
硃斌%趙喜聰%李楠%金巖
주빈%조희총%리남%금암
牙周再生%颌骨骨髓间充质干细胞%髂骨骨髓间充质干细胞%牙周膜干细胞%细胞聚合体%再生微环境
牙週再生%頜骨骨髓間充質榦細胞%髂骨骨髓間充質榦細胞%牙週膜榦細胞%細胞聚閤體%再生微環境
아주재생%합골골수간충질간세포%가골골수간충질간세포%아주막간세포%세포취합체%재생미배경
Periodontium regeneration%Jaw derived mesenchymai stem ceiis%Iiiac derived mesenchymai stem ceii%Periodontai iiga-ment derived stem ceii%Ceii aggregates%Regenerative microenvironment
目的:对比不同特异性组织来源的间充质干细胞的牙周再生能力. 方法:体外培养人牙周膜干细胞( human periodon-tai iigament stem ceiis,hPDLSCs),人颌骨来源的骨髓间充质干细胞(human jaw-derived bone marrow-derived mesenchymai stem ceiis,hJBMMSCs)和人髂骨来源的骨髓间充质干细胞(human iiiac-derived BMMSCs,hIBMMSCs),成骨诱导后进行成骨指标检测. 间接共培养JBMMSCs/IBMMSCs与PDLSCs,通过RT-PCR检测成骨相关基因,观察组织特异性来源的细胞间交互作用.构建JBMMSCs/IBMMSCs+PDLSCs细胞聚合体(ceii aggregates,CAs),检测成骨基因表达以及裸鼠皮下8 w异位牙周再生能力. 结果:经成骨诱导,hJBMMSCs的成骨相关指标表达均显著高于hIBMMSCs和hPDLSCs(P<0.05). 经JBMMSCs诱导的PDLSCs的成骨基因表达显著高于IBMMSCs(P<0.05);JBMMSCs+PDLSCs CAs成骨相关基因表达高于IBMMSCs+PDLSCs CAs(P<0.05),并且在裸鼠皮下异位再生牙周组织,前者形成排列规律的垂直于CBB表面的牙周膜样纤维,并且有更多新生骨基质沉淀. 结论:PDLSCs和JBMMSCs是具有组织特异性的间充质干细胞,是更合适牙周组织再生的种子细胞.
目的:對比不同特異性組織來源的間充質榦細胞的牙週再生能力. 方法:體外培養人牙週膜榦細胞( human periodon-tai iigament stem ceiis,hPDLSCs),人頜骨來源的骨髓間充質榦細胞(human jaw-derived bone marrow-derived mesenchymai stem ceiis,hJBMMSCs)和人髂骨來源的骨髓間充質榦細胞(human iiiac-derived BMMSCs,hIBMMSCs),成骨誘導後進行成骨指標檢測. 間接共培養JBMMSCs/IBMMSCs與PDLSCs,通過RT-PCR檢測成骨相關基因,觀察組織特異性來源的細胞間交互作用.構建JBMMSCs/IBMMSCs+PDLSCs細胞聚閤體(ceii aggregates,CAs),檢測成骨基因錶達以及裸鼠皮下8 w異位牙週再生能力. 結果:經成骨誘導,hJBMMSCs的成骨相關指標錶達均顯著高于hIBMMSCs和hPDLSCs(P<0.05). 經JBMMSCs誘導的PDLSCs的成骨基因錶達顯著高于IBMMSCs(P<0.05);JBMMSCs+PDLSCs CAs成骨相關基因錶達高于IBMMSCs+PDLSCs CAs(P<0.05),併且在裸鼠皮下異位再生牙週組織,前者形成排列規律的垂直于CBB錶麵的牙週膜樣纖維,併且有更多新生骨基質沉澱. 結論:PDLSCs和JBMMSCs是具有組織特異性的間充質榦細胞,是更閤適牙週組織再生的種子細胞.
목적:대비불동특이성조직래원적간충질간세포적아주재생능력. 방법:체외배양인아주막간세포( human periodon-tai iigament stem ceiis,hPDLSCs),인합골래원적골수간충질간세포(human jaw-derived bone marrow-derived mesenchymai stem ceiis,hJBMMSCs)화인가골래원적골수간충질간세포(human iiiac-derived BMMSCs,hIBMMSCs),성골유도후진행성골지표검측. 간접공배양JBMMSCs/IBMMSCs여PDLSCs,통과RT-PCR검측성골상관기인,관찰조직특이성래원적세포간교호작용.구건JBMMSCs/IBMMSCs+PDLSCs세포취합체(ceii aggregates,CAs),검측성골기인표체이급라서피하8 w이위아주재생능력. 결과:경성골유도,hJBMMSCs적성골상관지표표체균현저고우hIBMMSCs화hPDLSCs(P<0.05). 경JBMMSCs유도적PDLSCs적성골기인표체현저고우IBMMSCs(P<0.05);JBMMSCs+PDLSCs CAs성골상관기인표체고우IBMMSCs+PDLSCs CAs(P<0.05),병차재라서피하이위재생아주조직,전자형성배렬규률적수직우CBB표면적아주막양섬유,병차유경다신생골기질침정. 결론:PDLSCs화JBMMSCs시구유조직특이성적간충질간세포,시경합괄아주조직재생적충자세포.
Objective:To compare the periodontai regeneration of different specific-tissue derived mesenchymai stem ceiis .Meth-ods:Human periodontai iigament stem ceiis ( hPDLSCs ) , human jaw-derived bone marrow-derived mesenchymai stem ceiis (hJBMMSCs) and human iiiac-derived bone marrow-derived mesenchymai stem ceiis(hJBMMSCs) were cuitured in vitro.After osteo-genic induction ,reiative osteogenic tests were performed .PDLSCs induced by JBMMSCs/IBMMSCs were tested for osteogenic expression by RTPCR.Ceii aggregates (CAs) of PDLSCs and JBMMSCs/IBMMSCs were compound.The osteogenic genes expression were tested;PDL regeneration in nude mice ectopic transpiantation was observed .Results:After osteogenic induction , reiative osteogenic tests of JBMMSCs were higher than those of IBMMSCs and PDLSCs .PDLSCs induced by JBMMSCs expressed stronger osteogenic genes than those by IBMMSCs .CAs of PDLSCs/JBMMSCs expressed stronger osteogenic genes ,made more reguiar and verticai inserting fibers into the surface of CBB,regenerated more new bone matrix than CAs of PDLSCs /IBMMSCs.Conclusions:JBMMSCs and PDLSCs are spe-cific-tissue MSCs and more appropriate for PDL regeneration .
