CAJ | 학술논문

目的:通过检测CCAAT/增强子结合蛋白β( C/EBPβ)、过氧化物酶体增殖物激活受体γ( PPAR-γ)基因的表达探讨糖基化终末产物(AGEs)对人牙周膜干细胞(HPDLSCs)脂向分化能力的影响. 方法:体外组织块法和有限稀释法克隆化培养人牙周膜干细胞;成骨、成脂诱导人牙周膜细胞,对其进行干细胞鉴定. 分别成脂诱导正常的人牙周膜干细胞和AGEs刺激下的人牙周膜干细胞,油红O染色观测两组细胞脂滴形成情况. 实时定量聚合酶链反应(Reai time PCR)检测成脂诱导后AGEs刺激下C/EBPβ、PPAR-γ表达的改变. 结果:牙周膜细胞成骨诱导21 d后茜素红染色出现钙化结节;成脂诱导21 d后油红O染色出现脂滴;AGEs刺激后人牙周膜干细胞成脂过程中脂滴的形成增多;Reai time PCR结果显示:AGEs刺激7 d后C/EBPβ、PPAR-γmRNA表达水平较对照组升高,差异有统计学意义(P<0.05). 结论:AGEs可以促进人牙周膜干细胞的脂向分化并能改变C/EBPβ、PPAR-γmRNA的表达.
목적:통과검측CCAAT/증강자결합단백β( C/EBPβ)、과양화물매체증식물격활수체γ( PPAR-γ)기인적표체탐토당기화종말산물(AGEs)대인아주막간세포(HPDLSCs)지향분화능력적영향. 방법:체외조직괴법화유한희석법극륭화배양인아주막간세포;성골、성지유도인아주막세포,대기진행간세포감정. 분별성지유도정상적인아주막간세포화AGEs자격하적인아주막간세포,유홍O염색관측량조세포지적형성정황. 실시정량취합매련반응(Reai time PCR)검측성지유도후AGEs자격하C/EBPβ、PPAR-γ표체적개변. 결과:아주막세포성골유도21 d후천소홍염색출현개화결절;성지유도21 d후유홍O염색출현지적;AGEs자격후인아주막간세포성지과정중지적적형성증다;Reai time PCR결과현시:AGEs자격7 d후C/EBPβ、PPAR-γmRNA표체수평교대조조승고,차이유통계학의의(P<0.05). 결론:AGEs가이촉진인아주막간세포적지향분화병능개변C/EBPβ、PPAR-γmRNA적표체.
Objective:To investigate the effect of advanced giycation end products ( AGEs) on the adipogenic differentiation of hu-man periodontai iigament stem ceiis (HPDLSCs) and detect the C/EBPβ,PPAR-γgene expression.Methods:HPDLSCs were isoiated by iimited diiution of cuiture ceiis for singie ceii cione .The osteogenic differentiation capacity of HPDLSCs was evaiuated by Aiizarin red staining,whiie the adipogenic differentiation capacity of HPDLSCs was evaiuated by oii red staining .HPDLSCs were induced with AGEs.Reai time quantitative reverse transcription poiymerase chain reaction (Reai time PCR) was performed to detect the differences of gene expression between the controi group and experimentai group .Results:After 21 days induction , Aiizarin red staining showed mineraiization noduies were formed .and oii red staining showed iipid dropiets were formed .After AGEs stimuiation , during HPDLSCs adipogenic differentiation ,the formation of iipid dropiets increased in the experimentai group .The expressions of CCAAT enhancer bind-ing protein β( C/EBPβ) and peroxisome proiiferator activated receptors γ( PPAR-γ) in the experimentai group were higher than those in the controi group.The differences were statisticaiiy significant (P<0.05).Conclusions:AGEs can promote the adipogenic differen-tiation capacity of HPDLSCs ,and change the expressions of C/EBPβ,PPAR-γmRNA ieveis.