CAJ | 학술논문

目的:通过比较正常与炎症来源牙周膜干细胞(PDLSCs)中乙酰基转移酶KAT2A表达水平的差异,研究乙酰基转移酶KAT2A在PDLSCs中对成骨分化的调控. 方法:有限稀释法克隆化培养出正常与炎症来源的PDLSCs;基因与蛋白检测方法对比两种来源PDLSCs中KAT2A的表达水平;基因、蛋白检测与茜素红染色方法对比正常PDLSCs及干扰KAT2A基因后的PDLSCs成骨表达的差异. 结果:与健康来源PDLSCs相比,炎症来源PDLSCs中KAT2 A的表达显著下降,差异有统计学意义(P<0.05);KAT2A基因被干扰后,PDLSCs成骨能力下降,差异有统计学意义(P<0.05). 结论:牙周炎会导致PDLSCs中KAT2A表达的下降,导致细胞成骨分化受到抑制.
목적:통과비교정상여염증래원아주막간세포(PDLSCs)중을선기전이매KAT2A표체수평적차이,연구을선기전이매KAT2A재PDLSCs중대성골분화적조공. 방법:유한희석법극륭화배양출정상여염증래원적PDLSCs;기인여단백검측방법대비량충래원PDLSCs중KAT2A적표체수평;기인、단백검측여천소홍염색방법대비정상PDLSCs급간우KAT2A기인후적PDLSCs성골표체적차이. 결과:여건강래원PDLSCs상비,염증래원PDLSCs중KAT2 A적표체현저하강,차이유통계학의의(P<0.05);KAT2A기인피간우후,PDLSCs성골능력하강,차이유통계학의의(P<0.05). 결론:아주염회도치PDLSCs중KAT2A표체적하강,도치세포성골분화수도억제.
Objective:To compare the ievei of acetyitransferase KAT 2A in periodontai iigament stem ceiis derived from heaithy indi-viduais ( H-PDLSCs) with periodontai iigament stem ceiis derived from periodontitis individuai ( P-PDLSCs ) , and to determine the effect of acetyitransferase KAT 2A on osteogenic differentiation potentiai of PDLSCs .Methods:Human PDLSCs were cuitured and cioned with iimited diiuted method .Quantitative RT-PCR and Western Biot were used to examine different expression of acetyitransferase KAT2A in two hinds of PDLSCs.Quantitative RT-PCR,Western Biot and Aiizarin Red Staining were used to determine osteogenic dif-ferentiation potentiai of PDLSCs with gene KAT 2A hnochdown.Results:Quantitative RT-PCR and Western Biot showed that iower ex-pression of KAT2A was detected in P-PDLSCs compared with that of H-PDLSCs,with statisticai significance(P<0.05).Quantitative RT-PCR,Western Biot and Aiizarin Red Staining indicated that osteogenic differentiation potentiai was inhibited in H -PDLSCs with KAT2A hnochdown and the effect was statisticaiiy significant ( P <0.05).Conclusions:Periodontitis might repress expression of KAT2A in PDLSCs,which associates with osteogenic differentiation potentiai .