北方药学
北方藥學
북방약학
Journal of North Pharmacy
2015年
10期
5-6
,共2页
HPLC%杞菊地黄丸(浓缩丸)%马钱苷%含量测定
HPLC%杞菊地黃汍(濃縮汍)%馬錢苷%含量測定
HPLC%기국지황환(농축환)%마전감%함량측정
HPLC%Chrysanthemum rehmanrha pill%Loganin%Determination
目的:建立测定杞菊地黄丸(浓缩丸)中马钱苷的含量测定方法.方法:HPLC法;C18柱(4.6mm×250mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液(5:9:86),流速为1.0mL/min;检测波长236nm;柱温30℃;进样量10μL. 结果:马钱苷的线性范围为2μg~0.8416μg,相关系数为0.9998;平均加样回收率为99.0%,RSD为1.2%(n=6). 结论:该法简单,准确度高,重复性好,可用于控制杞菊地黄丸(浓缩丸)的质量.
目的:建立測定杞菊地黃汍(濃縮汍)中馬錢苷的含量測定方法.方法:HPLC法;C18柱(4.6mm×250mm,5μm),流動相為甲醇-乙腈-0.1%燐痠溶液(5:9:86),流速為1.0mL/min;檢測波長236nm;柱溫30℃;進樣量10μL. 結果:馬錢苷的線性範圍為2μg~0.8416μg,相關繫數為0.9998;平均加樣迴收率為99.0%,RSD為1.2%(n=6). 結論:該法簡單,準確度高,重複性好,可用于控製杞菊地黃汍(濃縮汍)的質量.
목적:건립측정기국지황환(농축환)중마전감적함량측정방법.방법:HPLC법;C18주(4.6mm×250mm,5μm),류동상위갑순-을정-0.1%린산용액(5:9:86),류속위1.0mL/min;검측파장236nm;주온30℃;진양량10μL. 결과:마전감적선성범위위2μg~0.8416μg,상관계수위0.9998;평균가양회수솔위99.0%,RSD위1.2%(n=6). 결론:해법간단,준학도고,중복성호,가용우공제기국지황환(농축환)적질량.
Objective:To estabilish a method for the determination of of loganin in Qi Chrysanthemum rehmanrha pill (concentrated pill) Method:HPLC C18(4.6mm×250mm,5μm) ,column was used with methanol—acetonitrile—phosphate(0.1%)(5:9:86). The flow rate was 1.0mL/min. The detection wave length was 236nm. The column temperature was at 30℃, and the inject volume was 10μL. Results:The calibration curves showed good linearity in the range of 2μg~0.8416μg (loganin)(r=0.9998).The average recoveries were 99.0%,RSD were1.2%(n=6).Conclusion:This Method is simple, accurate, and could be adopted for the quality control of this durg .
