CAJ | 학술논문

目的:建立鹅不食草中有效成分短叶老鹤草的定量分析方法. 方法:采用反相高效液相色谱法测定含量,色谱柱为Kromasil C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(45:55),流速:1.0mL/min;柱温:30℃;进样量:10μL;检测波长:225nm.结果:短叶老鹤草在0.010065~0.30195mg/mL范围内线性关系良好(r=1.000), 平均回收率(n=6) 为99.53%, 样品含量范围为0.048%~0.43%. 结论:建立的方法简单灵敏,准确可靠,重复性好,可用于鹅不食草的质量控制.
목적:건립아불식초중유효성분단협로학초적정량분석방법. 방법:채용반상고효액상색보법측정함량,색보주위Kromasil C18색보주(250mm×4.6mm,5μm),류동상위을정-수(45:55),류속:1.0mL/min;주온:30℃;진양량:10μL;검측파장:225nm.결과:단협로학초재0.010065~0.30195mg/mL범위내선성관계량호(r=1.000), 평균회수솔(n=6) 위99.53%, 양품함량범위위0.048%~0.43%. 결론:건립적방법간단령민,준학가고,중복성호,가용우아불식초적질량공제.
objective:To establish quantitative analysis of brevifolin in CENTIPEDAE HERBA. Methods:Quantitative analysis was performed on Kromasil C18 (250mm×4.6mm,5μm).The mobile phase was acetonitrile-water (45:55)at the flow rate of 1.0mL·min-1,the detection wavelength was225nm,the column temperature was 30℃.Results:Brevifolin showed good linear regression(r=1.000)within test ranges of 0.010065~0.30195mg/mL.The average recovery (n=6)was 99.53% ,and the range of contents in the samples were 0.048%~0.43% . Conclusion:The methods developed are sensitive ,reliable and useful for the analysis of samples purchased from different areas and can be used for the quality control of CENTIPEDAE HERBA.