北方药学
北方藥學
북방약학
Journal of North Pharmacy
2015年
10期
2-4
,共3页
高效液相色谱法%肾益康胶囊%大黄酚%大黄素
高效液相色譜法%腎益康膠囊%大黃酚%大黃素
고효액상색보법%신익강효낭%대황분%대황소
HPLC%Shenyikang Capsules%Chrysophanol%Emodin
目的:建立测定肾益康胶囊中大黄酚和大黄素含量的高效液相色谱法. 方法:色谱柱为SB-C18柱(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液(85:15),流速1.0mL/min,检测波长254nm,柱温30℃.结果:大黄酚在1.996~19.96μg(r=0.9995)范围内与峰面积线性关系良好,平均回收率为98.20%,RSD为1.63%(n=6). 大黄素在4.18~41.8μg(r=0.9996)范围内与峰面积线性关系良好,平均回收率为98.25%,RSD为1.78%(n=6). 结论:该方法简便、快速、准确,可较好控制该制剂的质量.
目的:建立測定腎益康膠囊中大黃酚和大黃素含量的高效液相色譜法. 方法:色譜柱為SB-C18柱(150mm×4.6mm,5μm),流動相為甲醇-0.1%燐痠溶液(85:15),流速1.0mL/min,檢測波長254nm,柱溫30℃.結果:大黃酚在1.996~19.96μg(r=0.9995)範圍內與峰麵積線性關繫良好,平均迴收率為98.20%,RSD為1.63%(n=6). 大黃素在4.18~41.8μg(r=0.9996)範圍內與峰麵積線性關繫良好,平均迴收率為98.25%,RSD為1.78%(n=6). 結論:該方法簡便、快速、準確,可較好控製該製劑的質量.
목적:건립측정신익강효낭중대황분화대황소함량적고효액상색보법. 방법:색보주위SB-C18주(150mm×4.6mm,5μm),류동상위갑순-0.1%린산용액(85:15),류속1.0mL/min,검측파장254nm,주온30℃.결과:대황분재1.996~19.96μg(r=0.9995)범위내여봉면적선성관계량호,평균회수솔위98.20%,RSD위1.63%(n=6). 대황소재4.18~41.8μg(r=0.9996)범위내여봉면적선성관계량호,평균회수솔위98.25%,RSD위1.78%(n=6). 결론:해방법간편、쾌속、준학,가교호공제해제제적질량.
Objective:To establish the HPLC method for determining the content of chrysophanol and emodin in Shenyikang Capsules. Methods:The HPLC method was used with the SB-C18 column (150mm×4.6m,5μm), The mobile phase consisted of methanol-0.1%phosphoric acid solution(85:15), The flow rate was 1.0mL/min, the detection wavelength was 254 nm, and the column temperature was 30℃. Results:The calibration curve of chrysophanol was linear in the range of 1.996~19.96μg(r=0.9995) .The average recovery rate was 98.20%,RSD=1.63%(n=6). The calibration curve of emodin was linear in the range of 4.18~4.18μg (r=0.9996).The average recovery rate was 98.25%, RSD =1.78% (n=6). Conclusion:The method is simple, rapid and accurate, And it can control the quality of the preparation. effectively.
